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Lycopene production from synthetic medium by Blakeslea trispora NRRL 2895 (+) and 2896 (−) in a stirred-tank fermenter

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Abstract

The dissolved oxygen tension of 20% of air saturation, pH-shift from 4.0 to 5.5 on day 3, and a moderate shear stress (calculated as an impeller tip speed, \( V_{\text{tip}} = 0. 9 2 6- 2. 1 6 1 \, {\text{m}}/{\text{s}} \)) were identified to be the key factors in scaling-up the mated fermentation of Blakeslea trispora NRRL 2895 (+) and 2896 (−) for lycopene production from a shake flask to a stirred-tank fermenter. The maximal lycopene production of 183.3 mg/L was obtained in 7.5-L stirred-tank fermenter, and then the mated fermentation process was successfully step-wise scaled-up from 7.5- to 200-L stirred-tank fermenter. The comparability of the fermentation process was well controlled and the lycopene production was maintained during the process scale-up. Furthermore, with the integrated addition of 150 μmol/L abscisic acid on day 3, 0.5 g/L leucine and 0.1 g/L penicillin on day 4, the highest lycopene production of 270.3 mg/L was achieved in the mated fermentation of B. trispora in stirred-tank fermenter.

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Acknowledgments

Financial support from the National Natural Science Foundation of China (NSFC, Project Nos. 20976038 and 21176059), the Key Project of Chinese Ministry of Education (Project No. 210132), Hubei Provincial Natural Science Foundation for Innovative Research Team (Project No. 2008CDA002), Scientific Research Key Project of Hubei Provincial Department of Education (Project No. Z20101401), Discipline Leader Project of Wuhan Municipality (Project No. 200951830553), Key Technology R&D Program of Wuhan Municipality (Project No. 201120822280-2), the Open Project Programs for the Key Laboratory of Fermentation Engineering (Ministry of Education), the National Key Laboratory of Biochemical Engineering (Project No. 2010KF-06), and the State Key Laboratory of Bioreactor Engineering are gratefully acknowledged. Ya-Jie Tang also thanks the Chutian Scholar Program (Hubei Provincial Department of Education, China) (2006).

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Authors and Affiliations

  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, 430068, Wuhan, China

    Xiu-Ji Liu, Rui-Sang Liu, Hong-Mei Li & Ya-Jie Tang

  2. Lab of Synthetic Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 201203, Shanghai, China

    Ya-Jie Tang

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  1. Xiu-Ji Liu
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  2. Rui-Sang Liu
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Correspondence to Ya-Jie Tang.

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Liu, XJ., Liu, RS., Li, HM. et al. Lycopene production from synthetic medium by Blakeslea trispora NRRL 2895 (+) and 2896 (−) in a stirred-tank fermenter. Bioprocess Biosyst Eng 35, 739–749 (2012). https://doi.org/10.1007/s00449-011-0654-4

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  • DOI: https://doi.org/10.1007/s00449-011-0654-4

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