Abstract
Diesel fuel storage tanks are not hostile environments for microorganisms and tend to form sludges in the water deposited at the bottom of the tanks. The lack of nutrient, carbon and energy limitations within these habitats boost the abundance and the metabolic activity of microorganisms providing microbial hotspots with high growing rates of diesel degradation (0.10 ± 0.021 d−1). Five different Phyla (Thermotogae, Spirochaetes, Firmicutes, Bacteroidetes Proteobacteria) were identified within the aqueous/sludge phase from in situ diesel storage tanks, by cultured independent molecular surveys using the 16S rDNA gene fragment. The identified dominant strains were Geotoga aestuarianus, Flavobacterium ceti, Spirochaeta thermophila, Propionispira arboris, Sporobacterium olearium and Dysgonomonas genera. The altitude where the storage tanks are located and the organic carbon concentration within the aqueous/sludge phases affected the bacterial diversity. Therefore, the more diverse the microbial communities are, the more probability of the presence of bacteria with capacity to metabolized diesel and eliminate organic matter. Despite, only phosphate showed an effect on the bacterial distribution within the storage tanks, there was an apparent lack of deterministic process in structuring microbial communities. Consequently, preventative protocols are a priority to avoid the microbial growth within diesel fuel storage tanks. A new focus of this worldwide problem within the oil industry would be to explore deeply the wide range of metabolic and adaptive capacities of these microorganisms. These microbial consortia are potential tools with new specific services to apply in bioremediation among others.
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Abbreviations
- DGGE:
-
Denaturing gradient gel electrophoresis
- NGS:
-
Next generation sequencing
- MPN:
-
Most probably number technique
- DUB:
-
Diesel uncultured bacteria
- NJ:
-
Neighbor-joining method
- DCA:
-
Detrended correspondence analysis
- CCA:
-
Canonical correspondence analysis
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Authors are deeply indebted to reviewers for their constructive criticism and helpful about the manuscript. Thanks, are given to Clara Santos for helping during the experimental phase of the work.
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This work was financed by Repsol, S.A.
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González-Benítez, N., Bautista, L.F., Simarro, R. et al. Bacterial diversity in aqueous/sludge phases within diesel fuel storage tanks. World J Microbiol Biotechnol 36, 180 (2020). https://doi.org/10.1007/s11274-020-02956-6
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DOI: https://doi.org/10.1007/s11274-020-02956-6