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Aerobic metabolism and oxidative stress tolerance in the Lactobacillus plantarum group

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Abstract

Aerobic metabolism and response to oxidative stress and starvation were studied in 11 Lactobacillus plantarum, L. paraplantarum and L. pentosus strains in order to assess the impact of aerobic metabolism on the growth and on the stress response. The strains were grown in aerobiosis without supplementation (AE), with hemin (AEH) or with hemin and menaquinone (AEHM) supplementation and in anaerobiosis (AN) in a complex buffered substrate. Growth rate, biomass yield, glucose and O2 consumption, production of lactic acid and H2O2, catalase activity, oxidative and starvation stress tolerance were evaluated. Aerobic growth increased biomass yield in late stationary phase. Further increase in yield was obtained with both hemin (H) and menaquinone (M) addition. With few exceptions, the increase in biomass correlated with the decrease of lactic acid which, however, decreased in anaerobic conditions as well in some strains. Addition of H or H + M increased growth rate for some strains but reduced the duration of the lag phase. H2O2 production was found only for aerobic growth with no supplementation due to catalase production when hemin was supplemented. To our knowledge this is the first study in which the advantages of aerobic growth with H or H + M in improving tolerance of oxidative stress and long-term survival is demonstrated on several strains of the L. plantarum group. The results may have significant technological consequences for both starter and probiotic production.

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Acknowledgments

This work was partly funded by Ministero dell’Istruzione, dell’Università e della Ricerca, Rome, Italy, PRIN n. 20088SZB9B.

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Authors and Affiliations

  1. Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano, 10, 85100, Potenza, Italy

    A. Guidone, R. G. Ianniello, A. Ricciardi & E. Parente

  2. Istituto di Scienze dell’Alimentazione, CNR, Avellino, Italy

    T. Zotta & E. Parente

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  1. A. Guidone
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  2. R. G. Ianniello
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  3. A. Ricciardi
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  4. T. Zotta
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  5. E. Parente
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Correspondence to A. Guidone.

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Guidone, A., Ianniello, R.G., Ricciardi, A. et al. Aerobic metabolism and oxidative stress tolerance in the Lactobacillus plantarum group. World J Microbiol Biotechnol 29, 1713–1722 (2013). https://doi.org/10.1007/s11274-013-1334-0

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  • DOI: https://doi.org/10.1007/s11274-013-1334-0

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