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High-Level Expression of Heme-Dependent Catalase Gene katA from Lactobacillus Sakei Protects Lactobacillus Rhamnosus from Oxidative Stress

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Abstract

Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H2O2), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H2O2 through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product fermentation, but they are deficient in catalase. In this study, the effect of heterologous expression of L. sakei catalase gene katA in L. rhamnosus on its oxidative stress resistance was tested. The recombinant L. rhamnosus AS 1.2466 was able to decompose H2O2 and the catalase activity reached 2.85 μmol H2O2/min/108 c.f.u. Furthermore, the expression of the katA gene in L. rhamnosus conferred enhanced oxidative resistance on the host. The survival ratios after short-term H2O2 challenge were increased 600 and 104-fold at exponential and stationary phase, respectively. Further, viable cells were 100-fold higher in long-term aerated cultures. Simulation experiment demonstrated that both growth and catalase activity of recombinant L. rhamnosus displayed high stability under environmental conditions similar to those encountered during sausage fermentation.

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Acknowledgments

We thank Professor Xinghua Guo (Institute of Microbiology Chinese Academy of Sciences) for providing Lactobacillus rhamnosus AS 1.2466 and Elisabeth Sørvig (Agricultural University of Norway) for the gift of plasmid pSIP502. This work was supported by National High-Tech R&D Program Grants (2007AA10Z354 and 2006AA10Z317) from the Ministry of Science and Technology of the People’s Republic of China.

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  1. Key Laboratory of Functional Dairy of Ministry of Education of the People’s Republic of China & Municipal Government of Beijing, College of Food Science & Nutritional Engineering, China Agricultural University, 17 Qing Hua East Road, Hai Dian District, Beijing, 100083, China

    Haoran An, Hui Zhou, Ying Huang, Guohong Wang, Chunguang Luan, Jing Mou, Yunbo Luo & Yanling Hao

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  1. Haoran An
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  2. Hui Zhou
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Correspondence to Yanling Hao.

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An, H., Zhou, H., Huang, Y. et al. High-Level Expression of Heme-Dependent Catalase Gene katA from Lactobacillus Sakei Protects Lactobacillus Rhamnosus from Oxidative Stress. Mol Biotechnol 45, 155–160 (2010). https://doi.org/10.1007/s12033-010-9254-9

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