Abstract
A novel Lactobacillus panis PM1 isolate was found to be capable of converting glycerol to 1,3-propanediol (1,3-PDO), an increasingly valuable commodity chemical. In this study the effects of various process parameters, including glucose and glycerol concentrations, inoculum size, temperature, aeration, pH, and carbon source were examined to determine the optimal conditions for the production of 1,3-PDO using a culture method simulating late log to early stationary phases. Inoculum size did not influence the production of 1,3-PDO, and temperature variance showed similar 1,3-PDO production between 25 and 37 °C under the examined conditions. Glycerol concentration and pH played a primary role in the final concentration of 1,3-PDO. The highest production occurred at 150–250 mM glycerol when 50 mM glucose was available. Alkaline initial conditions (pH 9–10) stimulated the production of 1,3-PDO which concurrently occurred with increased acetic acid production. Under these conditions, 213.6 mM of 1,3-PDO were produced from 300 mM glycerol (conversion efficiency was 71 %). These observations indicated that the production of 1,3-PDO was associated with the shift of the metabolic end-product ethanol to acetic acid, and that this shift resulted in an excess concentration of NADH available for the processing of glycerol to 1,3-PDO.
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Acknowledgments
We acknowledge the Saskatchewan Agriculture Development Fund and Agricultural Bioproducts Innovation Program of Agriculture and Agri-Food Canada for supporting this research. We thank Pound-Maker Agventures Ltd. for thin stillage samples. We are obliged to Jori Harrison for her assistance in the laboratory experiments. Ms. Sylvia Yada is also acknowledged for her careful editorial advice.
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Grahame, D.A.S., Kang, T.S., Khan, N.H. et al. Alkaline conditions stimulate the production of 1,3-propanediol in Lactobacillus panis PM1 through shifting metabolic pathways. World J Microbiol Biotechnol 29, 1207–1215 (2013). https://doi.org/10.1007/s11274-013-1283-7
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DOI: https://doi.org/10.1007/s11274-013-1283-7