Abstract
The cashew fruit (Anacardium occidentale L.) has been used as a promising agricultural resource for the production of low-molecular weight (MW) hyaluronic acid (HA) (104–105 Da). The cashew juice is a rich source of vitamin C containing, 1.2–2.0 g L−1. This work explores the effects of the initial concentration of the ascorbate on the solid fermentation of the juice-moisturized bagasse from the cashew apple fruit. The results show that the MW reduction of HA is proportional to the initial ascorbate concentration. The presence of ascorbate did not influence the Streptococcus zooepidemicus metabolism. However, the HA productivity was increased from 0.18 to 0.28 mg g−1 h−1 when the ascorbate concentration ranged from 1.7 to 10 mg mL−1. These findings contribute to the controlled production of HA in a low MW range, which is important in cell signalization, angiogenesis and nanoparticles production.
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Abbreviations
- ATCC:
-
American type culture collection
- BHI:
-
Brain heart infusion
- Da, kDa:
-
Daltons, kilodaltons
- HPLC:
-
High-pressure (performance) liquid chromatography
- HA:
-
Hyaluronic acid
- MW :
-
Molecular weight
- ROS:
-
Reactive oxygen species
- RID:
-
Refractive index detector
- SSF:
-
Solid-state fermentation
- μx :
-
Specific cell growth rate
- SF:
-
Submerged fermentation
- YACE/S :
-
Yield factor for acetate
- YX/S :
-
Yield factor for cell
- YP/S :
-
Yield factor for hyaluronic acid
- YP/X :
-
Yield factor for hyaluronic acid/cell competition
- YLAC/S :
-
Yield factor for lactate
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Acknowledgments
The authors acknowledge CAPES and FAPESP for the financial support, the Brazilian Microbial Collection for Environment and Industry (CBMAI) for the maintenance of the Streptococcus strain.
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de Macedo, A.C., Santana, M.H.A. Hyaluronic acid depolymerization by ascorbate-redox effects on solid state cultivation of Streptococcus zooepidemicus in cashew apple fruit bagasse. World J Microbiol Biotechnol 28, 2213–2219 (2012). https://doi.org/10.1007/s11274-012-1028-z
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DOI: https://doi.org/10.1007/s11274-012-1028-z