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Effect of ethanol and hydrogen peroxide on poly(3-hydroxybutyrate) biosynthetic pathway in Cupriavidus necator H16

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Abstract

Exposition of Cupriavidus necator to ethanol or hydrogen peroxide at the beginning of the stationary phase increases poly(3-hydroxybutyrate) (PHB) yields about 30%. Hydrogen peroxide enhances activity of pentose phosphate pathway that probably consequently increases intracellular ratio NADPH/NADP+. This effect leads to stimulation of the flux of acetyl-CoA into PHB biosynthetic pathway and to an increase of enzymatic activities of β-ketothiolase and acetoacetyl-CoA reductase while activity of PHB synthase remains uninfluenced. During ethanol metabolisation, in which alcohol dehydrogenase is involved, acetyl-CoA and reduced coenzymes NAD(P)H are formed. These metabolites could again slightly inhibit TCA cycle while flux of acetyl-CoA into PHB biosynthetic pathway is likely to be supported. As a consequence of TCA cycle inhibition also less free CoA is formed. Similarly with hydrogen peroxide, activities of β-ketothiolase and acetoacetyl-CoA reductase are increased which results in over-production of PHB. Molecular weight of PHB produced under stress conditions was significantly higher as compared to control cultivation. Particular molecular weight values were dependent on stress factor concentrations. This could indicate some interconnection among activities of β-ketothiolase, acetoacetyl-CoA reductase and PHB molecular weight control in vivo.

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Acknowledgments

This work has been supported by project MSM 0021630501 and FR 2613/G4/2009 of Czech Ministry of Education.

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Authors and Affiliations

  1. Faculty of Chemistry, Institute of Food Chemistry and Biotechnology, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Stanislav Obruca, Ivana Marova & Marie Stankova

  2. Faculty of Chemistry, Institute of Chemistry and Technology of Environmental Protection, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Ludmila Mravcova

  3. Research Institute of Brewering and Malting, Malting Institute Brno, Mostecka 7, 614 00, Brno, Czech Republic

    Zdenek Svoboda

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  1. Stanislav Obruca
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  2. Ivana Marova
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  3. Marie Stankova
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  4. Ludmila Mravcova
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  5. Zdenek Svoboda
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Correspondence to Stanislav Obruca.

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Obruca, S., Marova, I., Stankova, M. et al. Effect of ethanol and hydrogen peroxide on poly(3-hydroxybutyrate) biosynthetic pathway in Cupriavidus necator H16. World J Microbiol Biotechnol 26, 1261–1267 (2010). https://doi.org/10.1007/s11274-009-0296-8

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  • DOI: https://doi.org/10.1007/s11274-009-0296-8

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