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Recombinant production of Penicillium oxalicum PJ3 phytase in Pichia Pastoris

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Abstract

The 1,332 bp phytase gene of Penicillium oxalicum PJ3 was inserted into the expression vector, pPICZαA and expressed in the methylotrophic yeast, Pichia pastoris as an active, extracellular phytase. The recombinant phytase reached a maximum yield of 12 U/ml of medium at 120 h of cultivation after methanol induction under shake-flask conditions. The enzyme was glycosylated, with a molecular mass of about 62.5 kDa. The Michaelis constant (K m) and maximum reaction rate (V max) for sodium phytate was 0.37 mM and 526.3 U/mg of protein, respectively. The optimal activity occurred at pH 4.5 and 55°C.

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Abbreviations

YNB::

yeast nitrogen base with ammonium sulfate and without amino acids

YPD::

yeast extract peptone dextrose

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Acknowledgment

We acknowledge a graduate fellowship provided by the Ministry of Education through the Brain Korea 21 project.

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Authors and Affiliations

  1. School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, San 56-1 Sillim-dong, Gwanak-gu, Seoul , 151-742, Korea

    Jaecheon Lee, Yunjaie Choi, Peter-Changwhan Lee & Seungha Kang

  2. Inositol Signaling Group, LST, National Institute of Environmental Health Sciences, NIH, DHSS, Durham, NC, 27709, USA

    Jaiesoon Cho

  3. Choong Ang Biotech Co., Ltd., 833-6 Wonsi-Dong, Ansan-City, Kyunggi-Do, Korea

    Jinduck Bok

Authors
  1. Jaecheon Lee
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  2. Yunjaie Choi
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  3. Peter-Changwhan Lee
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  4. Seungha Kang
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  5. Jinduck Bok
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  6. Jaiesoon Cho
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Corresponding author

Correspondence to Jaiesoon Cho.

Additional information

Jaecheon Lee and Yunjaie Choi contributed equally to this work.

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Lee, J., Choi, Y., Lee, PC. et al. Recombinant production of Penicillium oxalicum PJ3 phytase in Pichia Pastoris . World J Microbiol Biotechnol 23, 443–446 (2007). https://doi.org/10.1007/s11274-006-9236-z

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  • DOI: https://doi.org/10.1007/s11274-006-9236-z

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