Abstract
Microorganisms in the soil that have been contaminated with heavy metals for a long-time exhibited resistance to heavy metals. In recent years, the use of resistant microbes and heavy metal-tolerant plants to repair heavy metal-contaminated soil has become an increasingly hot subject. In this study, heavy metal-tolerant bacteria ZG7 was isolated from the mining soils and injected into Bidens pilosa L. and Medicago sativa L.. The results revealed that: (1) Compared to the non-inoculated group, inoculation with ZG7 notably increased the dry weight and chlorophyll content in plants under heavy metal stress, as well as promoted the content of several types of antioxidant chemicals; (2) Combined remediation improved soil enzyme activities, with soil catalase, sucrase, and phosphatase activities remarkably increased by 136.06%, 124.17% and 180.14% in the ZG7- Bidens pilosa L.; And soil catalase, urease, and sucrase activities promoted by 68.07%, 130.08% and 239.79% in the ZG7- Medicago sativa L.; (3) The content of Pb and Zn in the ZG7- Medicago sativa L. combination soil were considerably lower (9.28% and 6.2%, respectively) than no strain group, but there was no substantial change in soil heavy metal content when combined with the Bidens pilosa L. remediation when compared to no strain group. In summary, the combination of strain ZG7 and plants had a good effect on the remediation of heavy metal contaminated soils, and ZG7- Medicago sativa L. had the best effect, which is a reliable technique for effective control of heavy metal pollution. This study provided theoretical support for microbial-phytoremediation joint remediation.
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The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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Funding
This work was financially supported by the Key Research and Development Program of Gansu Province (20YF3FA037) and Shaanxi Province (2020ZDLSF06-06, 2021NY-068), the Natural Science Basic Research Program of Shaanxi (2023-JC-QN-0245), the Xi’an Science and Technology Projects (22FWQY14), and the Special Plan of Education Department of Shaanxi Provincial Government (21JC028). We are grateful to all anonymous reviewers whose comments improved the quality of the manuscript. The authors also thank the Key Laboratory for ecological restoration and high-quality development of Qinling Mountains in the upper and middle reaches of the Yellow River, Xi’an Key Laboratory of Plant Stress Physiology and ecological restoration technology.
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Yueli Chen: Writing-original draft, Writing-review & editing. Formal analysis, Methodology, Investigation& editing. Mingbo Zuo: Writing-original draft, Writing-review & editing. Methodology, Investigation. Deng Yang: Methodology, Investigation, Writing-original draft. Yueqing He: Conceptualization, Validation. Haoming Wang: Writing-original draft, Validation. Xiaoxiao Liu: Writing-original draft, Writing-review & editing, Supervision. Minjuan Zhao: Validation, Visualization, Writing-Original Draft, Formal analysis. Lingling Xu: Validation. Jing Ji: Validation. Ying Liu: Validation, Methodology, Formal analysis. Tianpeng Gao: Conceptualization, Methodology, Visualization, Investigation, Writing-original draft, Writing-review & editing.
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Chen, Y., Zuo, M., Yang, D. et al. Synergistically Effect of Heavy Metal Resistant Bacteria and Plants on Remediation of Soil Heavy Metal Pollution. Water Air Soil Pollut 235, 296 (2024). https://doi.org/10.1007/s11270-024-07100-w
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DOI: https://doi.org/10.1007/s11270-024-07100-w