1 Introduction

The availability of high-quality freshwater with low turbidity is the most fundamental issue due to potential interaction with downstream treatment operations and negative impacts on customer acceptability (Mohseni-Bandpei et al., 2018). The turbidity of surface water changes according to the year’s season, mainly from agriculture and development activity, with high erosion rates due to silt and clay that can carry substantial amounts of pollutants (Igwe et al., 2017) (Razali et al., 2020). Water turbidity causes limited sunlight penetration into water bodies. Thus, it can harm fish and other aquatic life by reducing food supplies, degrading spawning beds, and affecting gill function (Matta et al., 2017).

Undesirable water turbidity not only causes public health problems but also creates an inadequate budget for water treatment processes because the safe drinking water supply should be free of turbidity (Anthony et al., 2021). Therefore, intake water from lakes and rivers should pass through treatment techniques to make it harmless and attractive to the consumers. Proper treatment is vital before usage (Kurniawan et al., 2012). The type of water treatment depends on the residue present in the water (Cui et al., 2020).

Various conventional methods, such as precipitation, adsorption, flotation, ion exchange, membrane filtration, and biological and electrolytic process, have been utilized to reduce and remove turbidity from water (Rajoria et al., 2022). Coagulation and flocculation are fundamental units in surface water treatment. Coagulation is the addition of synthetic or natural coagulants into the water to destabilize the negative charges of colloidal particles (Bhatia et al., 2007). Flocculation is a slow, gentle mixing of water to advance the flocs to grow to a bigger size to settle out quickly (Nan et al., 2016). Removing minute colloids depends on the amount of coagulant added.

Aluminum sulfate is the most widely used coagulant in water treatment because of its proven capability and lower cost. However, it creates by-products that harm human health (Wu et al., 2012); (Elsayed et al., 2021). Low-cost and safe coagulants with high cation capability are required to avoid this issue. Research in the past four decades has increasingly been directed toward producing natural coagulants that are renewable and nontoxic (Mumbi et al., 2018). The coagulant of Moringa oleifera seed extract (MOSE) is an alternative and is commonly used in less developed communities since it is easily biodegradable (Yamaguchi et al., 2021). The MOSE is feasible and applicable in separating colloidal particles because the extract components contain soluble cationic proteins, peptides, calcium, iron, vitamin C, and tocopherol that functions as a cationic polyelectrolyte (Dzuvor et al., 2022). Furthermore, the volume of settled sludge produced during the process can be used as bio-fertilizers to increase crop rates (Idris et al., 2016).

The rural communities in Iraq have freshwater scarcity. These areas suffer from conventional water treatment to treat seasonal variations in turbidity. Moreover, residents lack the expertise to remediate water quality using alum coagulants. Failure to use the alum dosage might cause Alzheimer’s disease. The use of MOSE is developed and expected to provide more advantages and is reported as low cost and safe to be consumed.

The research aims to utilize a safe and easy application of a low-cost natural coagulant of MOSE as a replacement for alum coagulant to evaluate and improve the water quality the residents in rural communities use for drinking water. The research focuses on the detailed dosage knowledge to treat various raw turbid water entering the areas. The study also gives clear instructions on how the dosages of MOSE (oiled and de-oiled) can neutralize and destabilize the suspension charge of turbid water and make water suitable for a drink.

2 Methodology

2.1 Water sample Preparation

Raw water turbidity samples ranging from 5–300 NTU were prepared using an ordinary China kaolin clay powder obtained from Chemical Drug House. A weight of 10 g of kaolin powder was suspended in a liter of tap water using a magnetic stirrer for half an hour to attain a homogenous raw sample. The kaolin suspension was allowed to settle down for more than an hour. Only supernatant is used for the coagulation experiments. Thus, colloidal particles are observed in water and used as a stock solution to prepare desirable turbid water samples after dilution practice. Prepared turbid water was kept and filled into clean 1.5-L polyethylene terephthalate bottles, and its turbidity was measured before coagulant addition.

2.2 Physiochemical Measurements of M. oleifera Coagulant

This research was carried out at the beginning of June month of 2021. The dried M. oleifera pods were collected from the Bakrajo agriculture field in Sulaymaniyah, Iraq (35°33′02.5″ N 45°20′27.1″ E). The coats and wings were removed and then crushed to a fine powder using a crusher and then sieved through a 250-μm sieve. To make the MOSE solution, 2 g of the seed was weighed and dissolved in 200 ml of distilled water in a beaker, followed by an hour of stirring and filtering through Whatman filter paper No. 42. To make de-oiled MOSE, 2 g of powdered seed was combined with 100 ml of 70% n-hexane as an extract solvent for 40 min. Subsequent separation of the residue from the supernatant occurred by centrifuging for 45 min at 4000 rpm. The supernatant was discarded, and the residual solid was dried at room temperature. From the dried sample, 2 g was mixed with 200 ml of distilled water. The suspensions were stirred for 40 min. The supernatant was filtrated through Whatman filter No. 1 after leaving the mixture to sediment totally. The filtrate was considered the de-oiled extract.

2.3 Coagulation Experiments

The experimental tests were implemented using a jar test apparatus coupled with six paddles (Model/Wagtech project, EN/ 15–810, Code/ F105AD109), as shown in Fig. 1. The beakers were well-washed and filled with a 1000-ml raw water sample after knowing its initial turbidity. The temperature of the water samples was 30 ± 2 °C. Different dosages 5–240 mg. L−1 of the extract was used for low, medium, and high typical initial turbid samples to obtain the optimum dosage required for turbidity removal. The jar apparatus underwent rapid mixing with the speed of 110 rpm for 1.5 min and then a slow mixing speed of 25 rpm for 25 min. Later, the suspensions were left for 30 min to settle down the floc formations in the beakers. The supernatants in beakers were collected for turbidity measurements from 20 cm beneath the water surface in each beaker using a pipette. The turbidity of water samples was measured as Nephelometric Turbidity Units NTU using the turbidimeter (Hanna turbidity meter HI93703). Each experiment run was performed twice to ensure the results’ accuracy.

Fig. 1
figure 1

Jar test—coagulation and flocculation process for surface water turbid

The value of pH, electrical conductivity, alkalinity, and hardness before and after treatment was measured. Figure 2 illustrates the process steps of coagulation for treating raw water samples with turbidity 100 NTU. The performance efficiency removal of MOSE coagulant was determined using the following equation:

Fig. 2
figure 2

A Raw water sample with initial turbidity of 100 NTU with 110 mg. L−1 of MOSE. B Aggregation of particles in flocculation step. C Settlement of flocs after 25 min. D dried solid flocs

$$\mathrm{\%\;Performance\;efficiency}=\frac{\mathrm{Initial\;turbidity}\left(\mathrm{NTU}\right)-\mathrm{Residual\;turbidity}(\mathrm{NTU})}{\mathrm{Initial\;turbidity}(\mathrm{NTU})}*100$$
(1)

Equation (1) does not take into account the decrease of turbidity in the negative control. The natural sedimentation of synthetic turbid samples in the absence of MOSE for 60 min was examined to show the natural settlement of colloidal particles in tap water and the main residual turbidity in the supernatant. One liter volume of synthetic initial turbidity samples of 5, 10, 15, 30, 50, 70, 100, 200, and 300 NTU was placed individually in a beaker. The water samples were allowed to settle down for 60 min. Samples were collected each 10 min from the surface of the water in the beakers to measure turbidity. Therefore, the natural sedimentation of kaolin colloidal particles should be deducted from the actual performance efficiency computed from Eq. (1) as shown below in Eq. (2):

$$\mathrm{Performance\;efficiency}=[\frac{\mathrm{Initial\;turbidity}\left(\mathrm{NTU}\right)-\mathrm{Residual\;turbidity }(\mathrm{NTU})}{\mathrm{Initial\;turbidity}(\mathrm{NTU})}-\mathrm{natural\;sedimentation}]*100$$
(2)

2.4 Floc Formation Kinetics

The Brownian motion of colloidal particles manages the process of coagulation at an early stage (Ricordel & Djelal, 2014). The motion weakens when colloidal particles become destabilized and agglomerate to a diameter greater than 1 µm (Ghernaout et al., 2015). The colloidal removal kinetic models were studied to examine and explain the flocculation mechanisms between the kaolin suspension and the component of MOSE. Kinetic studies were conducted to determine the removal rates and contact periods required to complete the coagulation process (Mohtar et al., 2019). The ability of M. oleifera to reduce the turbidity of the water sample is due to their coagulation, such as adsorption and neutralization of the positive charges with colloidal particles in the water (Tunggolou & Payus, 2017). Therefore, the colloidal particle adsorption of M. oleifera components U was then calculated using the following equation (Desta & Bote, 2021):

$$U=\frac{V \left({C}_{o}-{C}_{e}\right)}{W}$$
(3)

where Co and Ce represent the initial and final concentration of colloidal particles in a water sample, V represents the volume of the suspension sample, and W indicates the MOSE dosage.

2.4.1 Pseudo-First-Order Kinetic Model

Lagergren’s first-order expression describes the kinetic aggregation of colloidal particles with the component of MOSE coagulant. The approach is represented by the equation below (Mateus et al., 2018)

$$\mathrm{log}\left({U}_{e}-{U}_{t}\right)=\mathrm{log}\left({U}_{e}\right)-\frac{{K}_{1}t}{2.303}$$
(4)

where Ut (mg/g) is the floc formation with MOSE component at time t (min) and Ue (mg/g) is the equilibrium colloidal particle aggregated with positive ions in MOSE. The pseudo-first-order rate constant is k1 (min−1) (Bekhoukh et al., 2022). A plotting chart of log (Ue − Ut) versus t can yield the gradient (k1/2.303) and intercept (log Ue). Finally, the equilibrium colloidal particle uptake (Ue) and first-order kinetic constant (k1) could be determined.

2.4.2 Model of Pseudo-Second-Order Kinetics

The following equation represents the pseudo-second-order approach. The driving force for transferring colloidal particles (Ue − Ut) is proportional to the availability of positive ions in the added dosage of MOSE into the water sample (Bello et al., 2017)

$$\frac{t}{{U}_{t}}=\left(\frac{1}{{k}_{2}{ U}_{e}^{2}}+\frac{t}{{U}_{e}}\right)$$
(5)

where k2 is the second-order rate constant in (L. mg−1·min−1). Slope (1/Ue) and intercept 1/(k2 Ue2) are found from the relationship between t/Ut versus t.

2.5 FTIR Analysis

FTIR characterization was crucial to identify the effective main functional groups; present in the M. oleifera seeds that contribute to attracting the colloidal particles in the bulk solution. Seed powders were dried well in an oven set at 50 °C for 2 h. The dried seed was named and delivered to a chemical laboratory for FTIR examination.

2.6 Statistical Analysis

Design expert software (Version 13, Stat-Ease Inc., Minneapolis, USA) was used for the experimental design and analysis of the results. The significance of p < 0.05 was considered in all statistical analyses. The significance of ANOVA was used to verify the fitness of the regression model equation. Response surface methodology (RSM) was also applied to determine the effects of the MOSE concentration and initial turbidity variables on the turbidity removal efficiency.

3 Results and Discussion

The characteristics of the tap water used in the experiments are analyzed in the laboratory using the standard method (Federation and Association, 2005) and listed in Table 1.

Table 1 Tap water quality characteristics used for water experiments

The main physical properties of the prepared MOSE were tested in the laboratory, as shown in Table 2. To identify the chemical composition that formulated the solid seed coagulant structure, the XRF (X-ray fluorescence, Spectro IQ11/Ametek, materials analysis division/Germany) technique scan was used (Mohseni-Bandpei et al., 2018). The results illustrated in Table 3 show that the chemical oxides of SO3, P2O5, K2O, MgO, CaO, Na2O, and Al2O3 hold the highest percentages (6.214%, 1.643%, 0.9871%, 0.5613%, 0.2542%, 0.157%, and 0.1076%) respectively per 5 g of the seed powder. These functional chemicals give better attraction properties for enhancing the destabilization of colloidal particles from bulk liquid.

Table 2 The main physical properties of MOSE
Table 3 Chemical composition of M. oleifera seed powder

Table 3 shows the characteristics of the MOSE coagulant examined by the XRF technique confirming that MOSE is rich with numerous positive multivalent ions. The coagulant has the potential capability to destabilize the particles in turbid water through inter-particle bridging. This improves the treatability performance by increasing the agglomeration capacity of floc production during the procedures (Faye et al., 2017; Ribeiro et al., 2019).

Figure 3 illustrates that the settling velocity of colloidal particles was slow in the absence of a MOSE coagulant. As a consequence, removing turbidity from water is exceptionally difficult and could not achieve the treatment goals. Because of the presence of ions in the tap water quality, the colloidal settlement was consistent with the compression layer settling. An optimum dose of MOSE should be applied to destabilize and neutralize the negative charge of particles causing floc formations to improve the particle settling rate. Thus, acceptable turbidity removal could be achieved.

Fig. 3
figure 3

Natural sedimentation (no coagulant added) of colloidal particles of kaolin in tap water for low, medium, and high initial turbidity

The coagulation efficiency of MOSE for removing colloidal particles varies depending on the initial turbidity of water and the concentration of the added coagulant extract, as shown in Tables 4, 5, and 6. The performance efficiency of colloidal particles in the three tables was evaluated by considering the decrease in turbidity in the negative control. The investigation showed that the optimum dose for each turbidity range was essential because an overdose of the extract reduced the treatability performance and negatively affected the coagulation treatment process (Zhao et al., 2021). This caused a low reduction in colloidal removal due to the reversed net charge on the suspended solid particles in water (Nkalane et al., 2019). Furthermore, this overdosing resulted in the saturation of the polymer bridge sites and caused the re-stabilization of the particles due to insufficient particles to form more interparticle bridges (Muyibi & Evison, 1996). Therefore, these three tables’ results confirmed that increasing the coagulant dosage enhances treatability performance until agglomeration saturation is reached, whereby the performance starts to decline or stabilize. It was also demonstrated that tap water’s ionic composition greatly impacted the sorption of MOSE by colloidal particles or the configuration of the coagulant at the particle–water interface. Optimum doses of MOSE were investigated for different initial turbidity to give minimum residual turbidity. Tables 4, 5, and 6 illustrate that the optimum dose concentration of MOSE to reduce the initial turbidity of water samples 5, 10, 15, 30, 50, 70, 100, 200, and 300 NTU were 20, 50, 60, 40, 60, 80, 110, 200, and 230 mg. L−1 respectively with p < 0.05. As the initial turbidity of the water sample was increased, the required optimum dosage of MOSE also increased. The results of the effect of the initial turbidity on the turbidity reduction by MOSE are shown in Fig. 4. It was also observed that MOSE coagulant showed an exceptional performance efficiency reaching 89% ± 0.56, 80% ± 0.51, and 42% ± 0.41 for high, medium, and low initial turbidity of water samples, respectively. This confirmed the removal efficiency of water turbidity samples with M. oleifera coagulant is increased when water comes with dense suspended particles. However, its effectiveness declines with low turbid water. The reason is that the amount of oil in the seed extract forms a film coating that inhibits contact with the surface of the reaction and thus causes the formation of low-strength flocs (Muyibi et al., 2002).

Table 4 The effect of variation of MOSE dosages on low water turbid removal efficiency at 30 °C
Table 5 The effect of variation of MOSE dosages on medium water turbid removal efficiency at 30 °C
Table 6 The effect of variation of MOSE dosages on high water turbid removal efficiency at 30 °C
Fig. 4
figure 4

Turbidity reduction efficiency of MOSE for different initial turbidity of water at optimum dosage

All the experimental results in Tables 4, 5, and 6 revealed that the optimum dosage of M. oleifera seeds that gave the lowest residual turbidity depended on the water sample’s initial turbidity. Furthermore, when MOSE concentration exceeds the optimum dosage, the turbidity of water increases because all colloids have been neutralized and settled down with an optimum dosage. Thus, the excess coagulants cause turbidity in the water as they do not interact with oppositely charged colloidal particles (Shaarani et al., 2019).

The variables affecting the coagulation process were analyzed using response surface methodology (RSM). The variation of processing factor of both initial water turbidity and added MOSE concentration on turbidity reduction was investigated. Fifty-four experiment runs were generated using central composite design (CCD) during the analysis through Design-Expert software. The method of coding the variables of initial turbidity X1 and MOSE dosage concentration X2 was identified as reported in Table 7.

Table 7 Experimental factor codes and levels

In the study, the removal efficiency response of MOSE Y after the coagulation process was correlated with both independent variables of X1 and X2 in a quadratic regression model, as presented in Eq. 6. The significance of these two variables on the removal efficiency was evaluated, as shown in Table 8. It is noted that the X1 and X2 indicate the linear coefficient and effect of individual factor separately, whereas the variable X1X2 represent the interaction between those two factors, while the second-order terms X12 and X22 indicate their quadratic effects. The positive and negative signs indicate synergistic and antagonistic effects of the terms, respectively. The ANOVA result analysis in Table 8 shows that the regression model was significant, with an F-value of 99.26 and having a corresponding low p-value (p < 0.0001). The value of determination coefficient R2 of 0.9118 was achieved for the regression equation model which revealed a good fit for the experimental resulted data.

Table 8 ANOVA test for response surface quadratic model
$$Y=6.57675+0.404573 {X}_{1}+0.047671 {X}_{2}+1.395*{10}^{-3} {X}_{1}{X}_{2}- 1.313*{10}^{-3}{ X}_{1}^{2}-2.12*{10}^{-3} {X}_{2}^{2}$$
(6)

The low values of residual in Table 8 showed that the model fit was satisfactory, implying that approximately 90% of the dependent variable variance was ascribed to the independent factors and only around 10% of the overall variation could not be explained by the model. Furthermore, it is clear in the Table that the lack-of-fit term is not significant, the difference between adjusted and predicted R2 is less than 0.2, and the adequate signal-to-noise ratio is greater than 4 indicating that the model is significant. As a result, the developed model can be used to make theoretical predictions for the dependent variable of removal efficiency.

Figure 5 shows the correlation between the observed and predicted values obtained from the software. The figure also illustrates the residual typical plot, which reveals that the model has a few deviations from normal, suggesting a good fit. The figure clearly shows that all data points are distributed and very close along the 45-degree straight line, implying that the proposed quadratic model can positively predict removal efficiency response over independent variable inputs.

Fig. 5
figure 5

Actual and predicted value plot for the turbidity removal efficiency of water samples

Figure 6 illustrates a design expert surface plot exhibiting initial turbidity and MOSE dosage interaction effects on water turbidity decrease. The figure shows that initial water turbidity and MOSE concentration significantly impact the percentage removal rate of colloidal particles in water samples. In low-range turbidity, the dosage of MOSE is not potentially effective to achieve high removal efficiency. The figure also shows that over-dosing MOSE on low-range turbidity samples results in a lower reduction in removal rate due to the reversed net charge on the colloidal particles in water. Consequently, the excess dose has a negative impact on the coagulation treatment process, resulting in a poor reduction of colloidal removal. However, a steep increase in removal efficiency was noticed when the high initial turbidity of samples was treated with an adequate dosage of MOSE.

Fig. 6
figure 6

Design expert surface plot showing the interaction effects of both initial turbidity and MOSE dosage on the reduction of water turbidity

To improve coagulation, and achieve more efficient removal for low turbidity water, bioactive elements such as seed oil content were isolated and the active constituents of the extract were used (Vilaseca et al., 2014) as the results are shown in Table 9. The residual turbidity of water in this investigation can be further reduced if water flows through a filtration unit like a sand filter, it is easy for the community in rural areas to build such a filter unit. In the experiment tests, MOSE has been found as a potential clarifying agent in water treatment to neutralize and adsorb suspended particles present in the water.

Table 9 The effect of de-oiled MOSE dosages on low water turbid removal efficiency at 30 °C

In the experiment runs, no pH adjustment was carried out for the prepared initial samples as with MO coagulant there is no possibility of enhanced hydrophilicity as well as a reduction in the charge neutralization of the water molecules. This affirmed coalescing ability and electrostatic attraction between water and MOSE coagulant. Therefore, the treatability performance of the MOSE coagulant was not dependent on the pH value of the water solution (Shan et al., 2017). Tables 4, 5, 6, and 7 illustrate that the water solutions’ pH value before and after the experiments was quite stable. A similar result was obtained by Nhut et al. (2021).

In the investigation, it was observed that turbidity reduction with the application of MOSE improves agglomeration performance. The growth of flocs and agglomeration in the flocculation part was promoted, increasing the removal of colloidal matter in water. This is attributed to an enhancement in the floc size, with the performance of the coagulant in turbidity removal causing the rapid settling ability of the flocs due to coalescing of the colloids formed in suspension (Adelodun et al., 2020). Thus, the extent of aggregation and flocculation dynamics of colloidal particles in the experiments were observed shortly after the addition of the coagulants, whereby the destabilized particles aggregated to form larger flocs.

To understand the coagulation-flocculation kinetics, the extent of aggregation was monitored. This was carried out by investigating the effect of sedimentation time 45 min at a dosage of 50 mg. L−1—10 NTU, 60 mg. L−1—50 NTU, 210 mg. L−1—200 NTU. It was noticed that the concentration of colloidal solids decreased as the time of treatment increased. Figure 7 reveals that the optimum settling time to achieve turbidity removal efficiency for each run in the experimental tests was 30 min. This duration time for settling the floc formations is convenient and desirable due to the energy and time savings. Furthermore, a real clarification in water was noticed due to the activity of the extract with an increase in the settling time specifically at the beginning of the process.

Fig. 7
figure 7

Turbidity-time decay curves for three different initial turbidities of water at a convenient dosage of MOSE

The data for water turbidity reduction with respect to time for three ranges 10, 50, 200)NTU was fitted to kinetic Eqs. 4 and 5 to illustrate the best kinetic model in the removal of colloidal particles in water to form flocs as shown in Fig. 8. The resulting data were best fitted to a pseudo-first-order kinetic model with a high determinant coefficient, as shown in Table 10. A similar result was obtained by Ruelas-Leyva et al. (2017). The colloidal removal rate in the reaction kinetic is based on numerous internal and external factors such as dosage, and the characteristics of the water itself (Precious Sibiya et al., 2021). As the reaction proceeds, particles adhere themselves to both chemical and physical binding sites. The reaction lasts until the free sites are completely saturated, with rapid settling at first.

Fig. 8
figure 8

Fitting kinetic experimental data of three different raw water with models of a pseudo first order and b pseudo second order

Table 10 Kinetic model parameters for water samples treated with MOSE at 30 °C

Some physicochemical parameters for raw turbid water 100 NTU were measured before and after treatment with an optimum dosage of MOSE to reveal the extent of variation of water characteristics when treated with MOSE coagulant. The experimental results and ANOVA statistical analysis as shown in Tables 11 and 12 demonstrated that the treatment of water with crude MOSE showed little change in pH, electrical conductivity, and salinity. However, the alkalinity and hardness of water significantly decreased depending on the initial hardness and the added MOSE dosage (p < 0.05). This result was consistent with what was found by Muyibi and Evison (1996).

Table 11 Some physicochemical parameters at optimum dosage of MOSE for raw turbid water 100 NTU
Table 12 ANOVA test for physiochemical properties of turbid water 100 NTU coagulated with MOSE

Colloidal particle aggregation in turbid water is mainly based on the surface chemistry of MOSE (Araujo et al., 2010). Transmission FTIR spectra for the Moringa oleifera solid particle were obtained for identifying the effective surface functional groups as depicted in Fig. 9. The detected bands of 2856, 2926, and 3288 cm−1 are assigned to –OH stretching (Ogunmodede et al., 2021). The broad band centered at 3420 cm−1 is assigned to O–H stretching. The FTIR spectra of the seed showed a broad range of frequency approximately between 1666–2856 cm−1 and 3288–4000 representing the existence of free hydroxyl groups and bonded –OH bands of carboxylic acids. The sharp bending modes at peaks of 1666, 1542, 1239, and 1058 cm−1 are assigned to vibrations of groups. This functional group appears predominantly in the protein and fatty acid structures present in Moringa seeds. The peaks at 2923 cm−1 and 2852 cm−1 are assigned to symmetrical and asymmetrical stretching of the C–H of CH2 group present in fatty acids (Kizilkaya & Tekınay, 2014). In the region between 1666 and 1058 cm−1, there are intense bands assigned to C = O bond stretching. The spectra show the band at 1666 cm−1 associated with the amide group in the protein and the presence of a peak at 1542 cm−1 is assigned to C = N stretching and/or N–H deformation (Kizilkaya & Tekınay, 2014).

Fig. 9
figure 9

FT-IR spectrum of Moringa oleifera seeds, the maximum and minimum peaks obtained is shown in the boxes

4 Conclusions

The M. oleifera plant has a rapid growth rate with less duration time in rural areas, a simple technique to use its extract, and unskilled persons can easily and effectively employ this natural material without facing problems. Therefore, the coagulant of MOSE is locally available in large volumes and is feasibly economical. The results strongly supported the efficacy of M. oleifera as a better alternative to synthetic coagulants in water purification. The optimum MOSE dosage for the coagulation-flocculation process varies based on the initial water turbidity. Therefore, the high turbidity removal achieved in this study confirmed the potential of this important eco-friendly natural product potential for safely treating water in rural regions.