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Complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus

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Abstract

Brazil is one of the largest silk producers in the world. The domesticated silkworm (Bombyx mori) was formally introduced into the country in the twentieth century, and the state of Paraná is the main national producer. During larval stages, B. mori can be afflicted by many different infectious diseases, which lead to substantial losses in silk production. In this work, we describe the structure and complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus (BmNPV), the most important silkworm pathogen. The BmNPV-Brazilian isolate is a nucleopolyhedrovirus with singly enveloped nucleocapsids within polyhedral occlusion bodies. Its genome has 126,861 bp with a G + C content of 40.4 %. Phylogenetic analysis clustered the virus with the Japanese strain (BmNPV-T3). As expected, we have detected intra-population variability in the virus sample. Variation along homologous regions (HRs) and bro genes was observed; there were seven HRs, deletion of bro-e, and division of bro-a into two ORFs. The study of baculoviruses allows for a better understanding of virus evolution providing insight for biological control of insect pests or protection against the pernicious disease caused by these viruses.

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Acknowledgments

We thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support; Ingrid Gracielle Martins da Silva for kindly helping with the sample for TEM and SEM; and Jeffrey J. Hodgson for kindly reviewing the English writing style.

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Authors and Affiliations

  1. Laboratory of Baculovirus, Cell Biology Department, University of Brasília, Brasília, DF, 70910-900, Brazil

    Daniel Mendes Pereira Ardisson-Araújo, Fernando Lucas Melo, Miguel de Souza Andrade, Sônia Nair Báo & Bergmann Morais Ribeiro

  2. Laboratory of Functional Organization of the Nucleus, Cell Biology Department, Maringá State University, Maringá, PR, 85814-110, Brazil

    Rose Meire Costa Brancalhão

Authors
  1. Daniel Mendes Pereira Ardisson-Araújo
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  2. Fernando Lucas Melo
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  6. Bergmann Morais Ribeiro
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Correspondence to Bergmann Morais Ribeiro.

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Ardisson-Araújo, D.M.P., Melo, F.L., de Souza Andrade, M. et al. Complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus . Virus Genes 49, 477–484 (2014). https://doi.org/10.1007/s11262-014-1112-6

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