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Involvement of herpesviruses in cases of abortion among water buffaloes in southern Italy

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Abstract

A six-year study on water buffaloes from the Campania Region (Southern Italy) was conducted to evaluate the presence of bovine/bubaline herpesviruses in cases of abortion. A total of 244 buffalo foetuses were analysed by real-time PCR to detect the presence of: bovine alphaherpesvirus 1(BoHV-1), bubaline alphaherpesvirus 1 (BuHV-1), bovine alphaherpesvirus 2 (BuHV-2), and bovine gammaherpesvirus 4 (BoHV-4). The foetuses of 14 water buffaloes that showed abortions were positive for BuHV-1 (4 animals) and/or BoHV-4 (11 animals), with one of these cases showing co-infection with BuHV-1 and BoHV-4. This study reports the first identification of BoHV-4 in water buffaloes. Cases of abortion were analysed using both molecular and cultural assays for the presence of other pathogens. In nearly all the abortion cases positive for BoHV-4, the virus was identified as a co-infecting agent together with other microorganisms, whereas in two abortion cases, it was the only pathogen found.

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Acknowledgments

We would like to thank Mr. Gioacchino Scognamiglio and Mr. Pasquale Izzo for their support in database data extraction.

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  1. Claudia Esposito, Filomena Fiorito and Gianluca Miletti contributed equally to the study

Authors and Affiliations

  1. Zooprofilactic and Experimental Institute of Southern Italy, National Reference Centre on Water Buffalo Farming and Productions Hygiene and Technologies, Via Salute n. 2, Naples, Portici, Italy

    Claudia Esposito, Gianluca Miletti, Francesco Serra, Anna Balestrieri, Barbara Cioffi, Giorgio Galiero, Esterina De Carlo, Maria Grazia Amoroso & Giovanna Fusco

  2. Department of Veterinary Medicine, University of Naples Federico II, Via Delpino n. 2, Naples, Italy

    Filomena Fiorito & Claudia Cerracchio

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  1. Claudia Esposito
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Contributions

Conceptualisation: Claudia Esposito, Filomena Fiorito, Giorgio Galiero, Maria Grazia Amoroso, Giovanna Fusco; Methodology, Formal Analysis and Investigation: Claudia Esposito, Gianluca Miletti, Francesco Serra, Barbara Cioffi, Claudia Cerracchio, Anna Balestrieri, Maria Grazia Amoroso; Writing –review and editing: Claudia Esposito, Filomena Fiorito, Gianluca Miletti, Claudia Cerracchio, Maria Grazia Amoroso, Supervision: Filomena Fiorito, Esterina De Carlo, Giorgio Galiero, Maria Grazia Amoroso, Giovanna Fusco.

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Correspondence to Maria Grazia Amoroso.

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Esposito, C., Fiorito, F., Miletti, G. et al. Involvement of herpesviruses in cases of abortion among water buffaloes in southern Italy. Vet Res Commun 46, 719–729 (2022). https://doi.org/10.1007/s11259-022-09887-7

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