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Isolation and identification of bubaline herpesvirus 1 (BuHV-1) from latently infected water buffalo (Bubalus bubalis) from Iran

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Abstract

In order to isolate buffaloes herpesvirus 1 (BuHV-1) from latently infected water buffalo (Bubalus bubalis), 16 buffalo heifers were selected from a herd. At first, animals were bled and their sera were tested by virus neutralization (VN) test, using bovine herpesvirus 1 (BoHV-1). According to the results of VN test and dexamethasone injection (0.1 mg/kg BW) for 5 consecutive days, the examined buffaloes were divided into 4 groups. Vaginal and nasal swabs were daily collected from all buffaloes from day 0 to 10 days later. Based on the cytopathic effects in cell culture, a herpesvirus was isolated only from nasal swabs of three seropositive buffaloes which they had received dexamethasone. The nasal swabs of these three buffaloes were also positive in PCR, using primers specific for ruminant herpesviruses gD gene. The identity of the isolated viruses was determined according to partial amino acid sequences of gD, deduced from the nucleotide sequences of the PCR products. On the basis of sequence alignment, phylogenetic analysis, and genetic distances, the three buffalo virus isolates were more closely related to BuHV-1 and BoHV-5 than to BoHV-1.

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Funding

The financial support of the Vice Chancellor for research of the Shahid Chamran University of Ahvaz for grant is gratefully recognized.

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Authors and Affiliations

  1. Resident of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Negar Hedayat

  2. Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mohammad Rahim Haji Hajikolaei & Ali-Reza Ghadrdan Mashhadi

  3. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Masoud Reza Seyfi Abad Shapouri & Maryam Daghari

  4. Agricultural and Natural Resources Research and Education Center, Animal Science Improvement Research Department, Safiabad AREEO, Dezful, Iran

    Hamidreza Izadnia

Authors
  1. Negar Hedayat
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  2. Mohammad Rahim Haji Hajikolaei
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  3. Masoud Reza Seyfi Abad Shapouri
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  4. Ali-Reza Ghadrdan Mashhadi
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  5. Hamidreza Izadnia
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  6. Maryam Daghari
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Correspondence to Mohammad Rahim Haji Hajikolaei.

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This research was approved by the Ethics Committee of Shahid Chamran University of Ahvaz (ethics code: EE/97.24.3.3454/scu.ac.ir).

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Hedayat, N., Haji Hajikolaei, M.R., Seyfi Abad Shapouri, M.R. et al. Isolation and identification of bubaline herpesvirus 1 (BuHV-1) from latently infected water buffalo (Bubalus bubalis) from Iran. Trop Anim Health Prod 52, 217–226 (2020). https://doi.org/10.1007/s11250-019-02007-9

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  • DOI: https://doi.org/10.1007/s11250-019-02007-9

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