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Molecular detection of bovine alphaherpesvirus-1 in cases of reproductive disorders among cattle and buffaloes in Gujarat

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Abstract

Bovine alphaherpesvirus-1 (BoAHV-1) is an important viral pathogen that causes significant economic losses to the dairy industry. The present study aimed to determine the prevalence of BoAHV-1 in cases of bovine reproductive disorder. Clinical samples were collected from various villages in Gujarat using specialized FTA® cards and were tested using real-time PCR assay targeting the gB gene of BoAHV-1. Out of 401 animals, 18.20% (95% CI: 14.74–22.28%) tested positive for BoAHV-1 DNA. The percentage positivity of BoAHV-1 was 20.37% in abortion cases and 19.55% in retention of fetal membrane cases, while only one out of nine metritis cases screened in the study was positive for BoAHV-1 DNA. A higher percentage positivity in buffaloes (22.14%) compared to cattle (16.30%) was recorded, but this difference was not statistically significant (p = 0.169). The frequency of BoAHV-1 detection was higher among crossbreeds (16.76%) and exotics (19.61%) than among indigenous cattle (8.82%), although this difference was not statistically significant (p = 0.400). There was also no significant difference in frequency distribution among animals of varying parity, ranging from 15.20 to 33.33% (p = 0.540). This study confirms the widespread circulation of BoAHV-1 and highlights the need for its control and prevention.

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Acknowledgements

The authors are grateful to the management of the National Dairy Development Board (NDDB), Anand for providing necessary facilities and funding to carry out this work. The authors acknowledge the support provided by the veterinarians and staff of Anand Milk Union Limited (AMUL), Kutch Nav Nirman Abhiyan (KNNA) and Sabarmati Ashram Goushala (SAG) for collection and shipment of the spotted filter paper cards for this study under NDDB’s Brucella control project.

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National Dairy Development Board (NDDB), Anand.

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Authors and Affiliations

  1. National Dairy Development Board Research and Development Laboratory, IIL Campus, Gachibowli, Hyderabad, 500032, Telangana, India

    G. Tejaswi, R. V. Chandrasekhar Reddy, N. M. Ponnanna & L. N. Sarangi

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  1. G. Tejaswi
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  2. R. V. Chandrasekhar Reddy
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  3. N. M. Ponnanna
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  4. L. N. Sarangi
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Tejaswi, G., Reddy, R.V.C., Ponnanna, N.M. et al. Molecular detection of bovine alphaherpesvirus-1 in cases of reproductive disorders among cattle and buffaloes in Gujarat. VirusDis. 34, 526–530 (2023). https://doi.org/10.1007/s13337-023-00851-w

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