Abstract
The genus Vachellia (Fabaceae) has a pan-tropical distribution and numerous Vachellia species are currently observed to be expanding their indigenous ranges and/or are invasive. Most Vachellia species have the capacity to enhance nitrogen uptake via an N2-fixing rhizobial mutualism that manifests in specialized root nodule structures enabling the catalysis of atmospheric N2 into a plant useable form. Improved understanding of nodulation may provide new insight to the changing patterns of ecological success of Vachellia species. Here, we investigated how the seedling growth, allometry and nodulation of two common Vachellia species, the arid Vachellia erioloba and the mesic Vachellia sieberiana, responded to varied levels of water availability. Seedlings were grown at 4%, 8% and 16% soil moisture content (SMC) for four months. The seedling growth and allometry of V. erioloba was unresponsive to changing water availability, and no nodulation was observed. The allometry of V. sieberiana was responsive to changing water availability and nodulation was observed; with the highest nodule biomass and growth rate recorded at 4% SMC. These patterns suggest that V. erioloba does not require the rhizobial mutualism, possibly due to lower competitive interactions between woody plants and grass in the arid savanna. Whereas, due to the competitive vegetation interactions typical in the mesic savanna, N2- fixation via nodule development could provide V. sieberiana a competitive advantage over grass not only in limited N conditions, but also during periods of lower water availability.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Introduction
Savannas are open ecosystems characterised by discontinuous woody cover and a continuous herbaceous ground layer that is shade intolerant (Scholes and Archer 1997; House et al. 2003). They account for a fifth of the earth's land surface across four continents, including half of Africa (Scholes and Archer 1997; Sankaran et al. 2005). A major threat to savanna ecosystems is woody encroachment, defined as the increase in dominance and cover of woody species (Stevens et al. 2017; Devine et al. 2017). In Africa, woody cover is increasing at an average rate of ~ 2.4% per decade (Venter et al. 2018); threatening biodiversity, grazing provision, hydrology, and nutrient cycling (Parr et al. 2012; Honda and Durigan 2016; Lehmann and Parr 2016; Leitner et al. 2018). However, it remains unclear why only a small number of species are responsible for encroachment (Liu et al. 2013). Across Africa, the majority of encroaching woody species are species of Vachellia, Senegalia and Dichrostachys (Stevens et al. 2017). One hypothesis is that the ability to fix atmospheric nitrogen (N2) can contribute to this pattern (Stevens 2017). Indeed, many of these encroaching species have the capacity to fix N2 via a rhizobial mutualism (Sprent 1995; Scholes and Archer 1997). The rhizobial mutualism manifests via the formation of root nodules; where N2 is transformed into plant useable NH4 (Kambatuku et al. 2013).
Previous experiments have shown that the legume-rhizobia symbiosis is sensitive to climatic extremes. Globally, terrestrial N2-fixation has been found to be inhibited by increased drought (Dovrat and Sheffer 2019; Zheng et al. 2020). This is possibly because the mobility of rhizobia is impeded by low soil moisture (Deans et al. 1993), reducing symbiosis establishment (Swaine et al. 2007; Fall et al. 2011). However, drought has been found to trigger an 80% increase in nodule biomass in temperate forest tree Robinia pseudoacacia L. (Wurzburger and Miniat 2014). If the sensitivity of nodulation to drought is widespread it may explain why woody legumes that seldom nodulate, such as Vachellia erioloba (E.Mey.) P.J.H. Hurter, are more common within the arid savanna (< 450 mm mean annual precipitation (MAP); Fig. 1b). It has also been suggested that if N is not a limit on plant growth, the ability to nodulate is of limited advantage, as substantial energy must be invested in traits that facilitate survival in water limited conditions (Sprent and Gehlot 2010). Nodulating legumes, such as Vachellia sieberiana; (DC.) Kyal. & Boatwr., are more common in mesic savannas (~ 800–1000 mm MAP; Fig. 1c). Mesic savannas are typically N limited; due to high rates of soil nutrient leaching (Zahran 1999), competition with grasses (due to increased productivity associated with higher annual rainfall) (Cramer et al. 2007, 2010) and frequent fire events leading to N loss (Archibald and Hempson 2016; Pellegrini et al. 2016). Further, nodulation enables woody legume seedlings to compensate for limited soil N in a competitive environment at a critical, but vulnerable establishment phase (Kambatuku et al. 2013).
Savanna ecosystems are typified by bottlenecks in recruitment (Bond and Midgley, 2003). Particularly within semi-arid and arid savannas, water availability is the primary cause of a bottleneck at the seedling stage (Higgins et al. 2007; Stevens et al. 2014). Additionally, climate change predictions suggest that Africa will become drier and hotter, with changing patterns of seasonal rainfall and experience an increase in drought events (IPCC 2022). Encroachment is widespread but the drivers between mesic and arid landscapes are likely to differ. In arid savannas, maximum woody cover is limited by water availability so the potential for woody encroachment could be constrained (Sankaran et al. 2005). Whereas, within mesic savannas, there is sufficient water availability for canopy closure but disturbances from fire, herbivory and humans prevent canopy closure (Kraaij and Ward 2006). Therefore woody encroachment (and canopy closure) potential is high in mesic savannas (Osborne et al. 2018). However, it remains unclear whether drier conditions under climate change could slow or accelerate woody encroachment by either limiting or stimulating seedling growth.
In Africa, the woody component of savanna vegetation is often dominated by mimosoids Vachellia and Senegalia (Bouchenak-Khelladi et al. 2010). Previous research has identified that Vachellia contains the most aggressive encroachers (Lewis et al. 2021). Therefore, understanding how seedling growth and recruitment of Vachellia species responds to drier conditions at this critical demographic stage is useful. In particular, a seldom considered aspect of savanna seedling success is how lower water availability alters the functionality of the legume rhizobium symbiosis (Serraj 2003; Sankaran 2019). Previously it has been proposed that lower water availability can reduce N2-fixation by reducing carbon nodule metabolism, introducing oxygen limitation, thus causing reduction of N2-fixation product transport (Serraj 2003). Understanding the interactions between lower water availability and nodule production related to plant growth could help determine the functional role of N2-fixation in seedling success.
Here we investigated how soil moisture relates to the growth and nodulation response of two woody Vachellia species, one arid and one mesic, both considered encroachers in a controlled glasshouse experiment (Hauwanga et al. 2018; Russell et al. 2019). We asked (1) does water availability affect seedling growth rates? We expected that the growth of arid-adapted V. erioloba would be unaffected by extremes in water availability in comparison with mesic adapted V. sieberiana (Seymour 2003). We predicted that the aboveground growth V. sieberiana would be positively correlated with water availability (Kraaij and Ward 2006). (2) Does water availability affect nodulation? We predicted V. sieberiana would exhibit lower levels of nodulation (nodule biomass and nodule mass fraction) when grown in the lowest soil moisture treatment, as rhizobia movement would be limited (Swaine et al. 2007; Fall et al. 2011). The literature notes that nodulation in V. erioloba is rare with previous research suggesting this occurs as V. erioloba obtains N from ground water rather than N2-fixaton (Barnes et al. 1997). Therefore, we assumed that it would be unlikely that V. erioloba would develop nodules across any of the soil moisture treatments.
Materials and methods
Description of study species
Vachellia erioloba is herbivore dispersed and occurs in arid savannas where rainfall is less than ~ 450 mm MAP (Fig. 1a; Fig. 1b) (Seymour 2008). Nodulation is infrequent for the this species, this is postulated to be due to its long roots that are able to access mineral N in ground water (Barnes et al. 1997; Sprent 2009). V. erioloba is a drought resistant, slow growing species characteristic of acidic sandy soils (Seymour 2008), and is recognised as a mild encroacher (Stevens et al. 2017; Hauwanga et al. 2018). Recruitment is generally episodic, and wet season dependent (Seymour 2008). Within its native range herbivore densities are low and fire is infrequent (Barnes 2001; Seymour 2008).
Vachellia sieberiana is a herbivore-dispersed species found across southern Africa where rainfall ranges from 800-1100 mm MAP (Fig. 1a; Fig. 1c) (Bunney 2014) and is known to nodulate (Sprent 2009). V. sieberiana grows on deep, well-drained, light sandy and medium loamy acid soils (Tadesse et al. 2007). This species is fast growing (Sunmonu and Van Staden 2014), and considered to be a woody encroacher (Stevens et al. 2017; Russell et al. 2019). Browse pressure is primarily on adult trees by Giraffe camelopardalis (Zinn et al. 2007). Within V. sieberiana’s range, fire is frequent and fuelled by highly productive grasses (February et al. 2013; Bunney 2014).
Experimental design
Seeds were purchased from Silverhill Seeds (http://www.silverhillseeds.co.za) (Cape Town, South Africa). The seeds of both species had coat-imposed dormancy, and pre-germination treatments were required. Therefore, the seeds were soaked in sodium hypochlorite (NaClO) for two minutes, to reduce fungal and mould growth and then soaked in boiling water for 10 minutes (Cramer et al. 2007). Treated seeds were germinated in petri dishes containing 10% Agar gel placed in a growth chamber (Conviron A1000, Conviron Europe ltd, Isleham, Cambridgeshire, B7 5RJ, UK) at 30 °C for three-four days until germination occurred. There was approximately ~ 90% germination success in both species.
Seedlings were grown from May to September 2018 (winter) at Stellenbosch University’s glasshouses heated using two standard garden infra-red heaters mounted three metres apart across the glasshouse ceiling. The average glasshouse air temperature was 25 °C and soil temperatures were between 17 °C and 35 °C. Temperature was measured using Thermochron iButtons (Thermochron, Baulkham Hills, Australia).
The germinated V. erioloba and V. sieberiana seedlings were transplanted into individual two litre pots. The soil was a mixture of a native alluvial sand aggregate taken from the Kalahari Desert and vermiculate (two parts sand: one part vermiculate). Seedlings were randomly distributed in the glasshouse and were moved every three days to ensure a homogenised growing environment. In total, the experiment consisted of 180 plants of two species.
All seedlings were provided with 5 ml of Strake Ayres: Nutrifeed; a water soluble fertiliser during week one (Strake Ayres, South Africa) (Macronutrient quantities available in Appendix Table 3). The soil was not inoculated with rhizobia. As the origin of the sand was the Kalahari desert where V. erioloba is a keystone species we assumed that a compatible rhizobia would be present (van der Merwe et al. 2019). Given the limited understanding of in-situ growth traits of Vachellia species we chose to use present free-living rhizobia in the sand (Winters et al. 2018). We believed that this would provide an accurate representation of the natural ability of rhizobia to survive water limited conditions (Shetta 2015).
Soil moisture treatments
Three watering treatments were imposed (measured in volumetric soil moisture content), where for each species 30 individuals were watered at an average of 4% soil moisture content (SMC) (0.100 m3/m3); average 8% SMC (0.180 m3/m3); and average 16% SMC (0.280 m3/m3). During the first two weeks 200 ml of water was given to each pot every two days to ensure establishment (Kraaij and Ward 2006). During weeks three and four, seedlings received 200 ml water every three days. At the beginning of week five (approximately one month after being transplanted into individual pots) soil moisture treatments were applied.
The appropriate SMC for this experiment was determined through a pilot study involving already germinated seedlings Vachellia exuvialis (I.Verd.) Kyal. & Boatwr. Here, 10 V. exuvialis seedlings per water treatment were subjected to the following water treatments: 1% SMC, 2% SMC, 4% SMC, 6% SMC, 8% SMC, 14% SMC, 16% SMC, 18% SMC, and 20% SMC. On this basis, the soil moisture treatments applied in the experiment reported here were determined by recording the health and survival of V. exuvialis to a range of watering regimes for 30 days. In the pilot, we observed for signs of root rot and leaf longevity in the higher SMCs. We used V. exuvalis in the pilot study due to seed availability, and it is fast growing species with a high germination success rate. Supplementary information on metrics of performance of V. erioloba and V. sieberiana in relationship to water availability were gathered from the literature (Cramer et al. 2007; Shetta 2015; Azad and Sumon 2016, 2017).
Soil moisture was recorded throughout the main experiment and pilot study using an HS2 HydroSense II Display (Campbell Scientific Ltd, Loughborough, UK). Soil moisture readings were collected every three days and the pots were watered accordingly.
Plant and leaf measurements at harvest
Plants were harvested at three ages, providing ten replicates per species x water treatment x harvest. Ten seedlings of each species of each water treatment were harvested at three points. Harvests were at one (Harvest 1), two (Harvest 2) and three months (Harvest 3) post water treatment. Height (mm) of each seedling was measured weekly.
Seedlings were separated into above and belowground biomass, and roots were carefully washed to maintain fine root mass. Nodules were removed from the roots using forceps and cleaned using a paint brush. For each harvested plant, the final plant height, dry aboveground and belowground biomass (grams), and nodule dry biomass (grams) were recorded. We chose to focus upon nodule biomass rather than nodule count as this is keeping with the dominant approach in the literature (Voisin et al. 2003; Cramer et al. 2012; Menge et al. 2015). We also calculated nodule mass fraction using the following equation:
All plant material was oven dried at 65 °C for 36 hours (Kambatuku et al. 2013).
Statistical analyses
Linear mixed effect models were fitted to test the effect of water availability on height (mm) and absolute growth (mm) (lme4 package; Bates et al. 2015). Height and absolute growth measurements were log transformed prior to analysis. Week and water treatment were fitted as fixed effects, and individual tag number (Species-Treatment-Pot) was fitted as a random effect. Due to the large difference in the niches occupied by these two species they were separated when creating the model assessing height related to water treatment. Only height and absolute growth for individuals that were harvested during Harvest 3 for V. sieberiana and V. erioloba was used in this analysis. This was because we were not interested in the temporal response of plant growth.
The seedling biomass and allocation data [below ground biomass (grams), above: belowground ratio and aboveground biomass (grams)] were analysed using a two-way ANOVA (Car: Companion to Applied Regression package; Fox and Weisberg 2019). The data was normal in distribution. Post-hoc Tukey significant difference (HSD) tests were carried out to separate the effects of water availability on the biomass for each species (MulticompView: Visualizations of Paired Comparisons package; Graves et al. 2019). To check homogeneity of variance a Levene’s test was used as a robust test of deviations from normality (Car: Companion to Applied Regression package; Fox and Weisberg 2019). Only the biomass data that was collected during Harvest 3 for V. sieberiana and V. erioloba was used in this analysis. This was because we were not interested in the temporal response of plant growth.
A two-way ANOVA was used to explore whether there was a correlation between soil moisture content, harvest and nodulation (nodule biomass (grams), nodule mass fraction (grams) and nodule count), using the Car: Companion to Applied Regression package (Fox and Weisberg 2019). Nodule biomass (grams) and nodule mass fraction data was log transformed prior to analysis. A post-hoc Tukey significant difference (HSD) and a Levene’s test were also applied. Only nodulation data collected from V. sieberiana across all three harvesting efforts was used in this analysis. This decision was informed by the lack of nodulation displayed by V. erioloba. All figures were created using the ggplot2; elegant graphics for data package (Wickham 2016) All analyses was conducted in the R environment 3 5.1 (R Core Team 2020).
Results
Does water availability affect seedling growth rates?
The height and aboveground biomass of the four month-old V. sieberiana seedlings (collected in harvest 3) was affected by water treatment (Table 1; Table 2; Appendix Table 4). V. sieberiana seedlings grown in the driest conditions (4% SMC) were significantly taller (Fig. 2a; Table 1) and had the largest aboveground biomass (Fig. 3b; Appendix Table 5) than seedlings grown at 8% SMC and 16% SMC. In contrast, the belowground biomass of V. sieberiana was not affected by water availability (df = 2, F = 1.45, p > 0.050; Fig. 3b; Appendix Table 4).
The growth and allocation patterns of the arid V. erioloba did not vary across water treatment (df = 2, F = 1.45, p > 0.050; Fig. 3a; Fig. 3b; Appendix Table 5). Relative to V. sieberiana, V. erioloba seedlings were shorter (df = 2, p > 0.050; Fig. 2a; Table 1), but proportionally had a larger belowground biomass (df = 1, F = 57.42, p < 0.010; Fig. 3c; Appendix Table 4).
Does water availability affect nodulation?
As expected, V. erioloba did not develop nodules across any the water treatments. Nodule count and nodule biomass in V. sieberiana increased with age (df = 2, F = 129.194, p < 0.001; Fig. 4a; Appendix Table 5; Appendix Table 6). There was an 800% increase in the number of individuals that nodulated between Harvest 1 (two months old) and Harvest 2 (three months old) (Appendix Table 5). There was an 11% increase in the number of individuals that nodulated between Harvest 2 (three months old) and Harvest 3 (four months old) (Appendix Table 5).
Nodule mass fraction was affected by water treatment (df = 2, F = 3.098, p < 0.05; Appendix Table 5). Proportional to total plant biomass, V. sieberiana seedlings grown in 4% SMC and 8% SMC produced nodules with the largest biomass (Fig. 4c). Further, V. sieberiana seedlings grown at 4% SMC produced fewer nodules overall, but with the highest total nodule biomass. Whereas V. sieberiana seedlings grown at 16% SMC produced a higher number of nodules, with a lower nodule biomass than V. sieberiana seedlings grown at 4% SMC (Appendix Table 6).
Discussion
Does water availability affect seedling growth rates?
The growth of V. sieberiana seedlings increased with a decline in water availability, whereas the growth of V. erioloba was unresponsive. Correlations between reduced water availability and increased growth/success has been seen in other Vachellia species; rapid above and belowground growth in Vachellia tortilis and Vachellia raddiana has been reported during the dry season in Southern Israel (Winters et al. 2018). These two species are known to nodulate (Sprent 2009), and are native to the semi-arid (500–1000 MAP) savanna (Ludwig et al. 2001). In some Vachellia species, soil water deficits have been found to lower nutrient absorption due to decreased mobility of nutrients to the root surface (Moura and Vieira 2020). Under drought conditions, slow nutrient diffusion from the soil to the root surface reduces nutrient translocation to leaves (Vieira et al. 2019). Therefore, unless the plant responds with accessing additional sources of N photosynthetic rates and enzymatic activity could decline, resulting in a reduction in growth rates (Moura and Vieira 2020). In addition, it is possible that lower soil water availability may also signal a competitive environment, triggering investment in nodulation to enhance plant growth (Sprent et al. 2010; Foxx and Fort 2019). V. sieberiana is native to the mesic savanna, where vegetation structure is driven by competition between grass and trees (Sankaran et al., 2004). In this experiment, the increased growth in low water availability displayed by V. sieberiana could be explained by the lack of competition.
As predicted the growth and allometry of V. erioloba was unaffected by changing soil moisture. Plant growth rate traits are only one of many elements of species life history strategy. These traits must be considered alongside the ability to survive and reproduce under a range of environmental conditions (Adams et al. 2016). Arguably, flexible growth patterns that increase water uptake under water-stressful conditions would not benefit V. erioloba survival in arid environments. Therefore traits favouring slow growth that require limited water are possibly advantageous for persistence (Seymour 2003). In V. erioloba a lack of phenotypic plasticity in growth and allocation likely facilitates the growth of deep roots that in arid conditions enables deep water access, and thereby resilience to drought in environments where competition is unlikely to limit growth. The lack of phenotypic plasticity is further demonstrated as tap root construction is favoured even when water availability is not limiting (Seymour 2008; February et al. 2013) and it is a rigid pattern of belowground investment that allows mature individuals of V. erioloba to survive in water limited environments (Barnes 2001).
Does water availability affect nodulation?
Increases in V. sieberiana plant height and total biomass in the driest soil conditions (4% SMC) were correlated with an increase in nodulation expressed nodule biomass and nodule mass fraction. Similar patterns of increasing nodulation with plant biomass have been found in Albizia saman and Leucaena leucocephala (Azad et al. 2013). An increase in nodule biomass is indicative of increased N2-fixing bacteria concentration within the nodules (Gwata et al. 2004). Hence, increased activity likely to correlate with increased available N for plant growth. We propose this pattern of increased height in V. sieberiana under lower water availability may be mediated through nodulation. In this species, low water availability possibly signals a belowground competitive environment and further where water stress can result in plant tissue damage that triggers jasmonic acid responses that in turn triggers increased nodulation (Sun et al. 2006; Hause and Schaarschmidt 2009). In some cases, under such conditions, if a plant has sufficient C reserves to maintain the rhizobia mutualism there will be an increase in the N available for growth. Our findings were contrary to our original prediction as movement of rhizobia can be inhibited by a lack of water in the soil (Swaine et al. 2007; Fall et al. 2011) and highlights remaining uncertainties in understanding N2-fixation.
Vachellia sieberiana develops larger but fewer nodules within a drier environment. We assume the relationship between nodule count and biomass can be explained through the interaction of rhizobia mobility (Swaine et al. 2007; Fall et al. 2011) and root cell wall thickness associated with root plasticity in response to low soil moisture (Mantovani et al. 2015; Chaulagain and Frugoli 2021). Vachellia sieberiana has been found to develop a tap root when soil water availability is low (Mugunga and Sahinkuye 2020). Tap roots tend to have a thicker cell wall which can hinder rhizobia infection (Gavrin et al. 2017). This can reduce the number of nodules developed, as rhizobia will opt to infect the root via the already developed nodules (Shetta 2015). Therefore, it is possible that the flexible development of nodules, in response to root plasticity, could mean that drought is less of a limiting factor to seedling recruitment than previously hypothesized for V. sieberiana (Case et al. 2020).
Future research
Future projections suggest southern Africa will experience an increase in extreme weather events, such as drought (IPCC 2022). Our research gives some indication of how the seedling establishment phase of these encroaching species is impacted by water stress. Our results indicate that for an arid species such as V. erioloba, whose allometry remains unaffected by lower water availability, a hotter, drier climate may permit an extension of geographical range. However, the ability of V. sieberiana plants to nodulate and fix N2 could provide a competitive advantage in conditions where water availability is low. Some studies have reported V. sieberiana is extending to arid areas as a result of vegetation change (Mugunga and Sahinkuye 2020). However, the relationship between savanna tree cover and water availability is not straight-forward (Sankaran et al. 2005), as water availability interacts with fire and herbivory which can strengthen or weaken recruitment bottlenecks (Bond 2008). Further, the understanding of seedling, sapling and adult survival under prolonged drought is not well understood. We suggest future experiments examining water availability consider the interactive impacts of fire and herbivory on plant growth and nodulation in Vachellia. As this will give a more complete picture of the factors that characterise recruitment bottlenecks in savanna woody plants.
Within arid environments Vachellia species are thought to establish only in years of above-average rainfall (Seymour 2008), and it is likely changing rainfall will alter establishment and survival (Van Der Merwe et al. 2020). In this experiment, to allow seedlings the opportunity to establish they were exposed to four weeks of continuous watering before treatments were imposed. We suggest that future experiments should identify the minimum watering period for seedling establishment to understand how seedlings will adapt to these climatic changes.
Further, experiments testing the effects of water availability are difficult to execute in-situ as many factors influence how ecosystems experience change in precipitation (Vicca et al. 2012). The most important factor is not the amount of precipitation, but the amount of water that plants can access. This ‘plant available water’ is dependent on soil texture, and rooting depth (Tolk 2003) and estimations of the magnitude is often complicated by runoff water and stem flow (Fall et al. 2011). With this in mind, we suggest that future experiments take plant size into consideration when controlling soil moisture content. Further this experiment would have benefitted from the inclusion of measurements such as the 15N natural abundance method that has been successfully applied to quantify N2-fixation by legumes (Senthilkumar et al. 2021). We suggest that future experiments incorporate these measurements to provide a more in depth understanding of nodulation.
Conclusion
Water availability has a wide range of effects on closely related Vachellia species relative to their environmental niche and associated growth traits. For V. sieberiana to be successful in the mesic environment it requires extended periods of growth to escape fire and compete with grass. Nodulation could enable a flexible N supply to enhance growth over such time periods. In this experiment the increased nodulation of V. sieberiana associated with low water availability suggests nodulation potentially assists the withstanding of low water availability within its environmental niche. This trait could be useful during periods of drought. The growth traits of V. erioloba, remained unaffected by lower water availability, potentially attributable to its high tolerance to aridity (Barnes et al. 1997). Perhaps, the measure of success for V. erioloba is not rapid growth but being able survive in a water limited environment via methods of belowground investment. These patterns suggest that effective life history strategies for the arid-adapted species can preclude the requirement for a rhizobial mutualism, as typically soil N is relatively high and there are lower competitive interactions (Aranibar et al. 2004). Whereas in the mesic savanna, where soil N is scare there is higher competitive stress, woody plants may favour nodulation, especially under low water supply that limits root access to soil N (Aranibar et al. 2004; Veldhuis et al. 2016).
Data Availability
See online resources and Github folder: https://github.com/Elizabeth261191/Telford_et_al_2022
References
Adams MA, Turnbull TL, Sprent JI, Buchmann N (2016) Legumes are different: Leaf nitrogen, photosynthesis, and water use efficiency. Proc Natl Acad Sci U S A 113:4098–4103. https://doi.org/10.1073/pnas.1523936113
Aranibar JN, Otter L, Macko SA, Feral CJW, Epstein HE, Dowty PR, Eckardt F, Shugart HH, Swap RJ (2004) Nitrogen cycling in the soil-plant system along a precipitation gradient in the Kalahari sands. Glob Chang Biol 10:359–373. https://doi.org/10.1111/j.1365-2486.2003.00698.x
Archibald S, Hempson GP (2016) Competing consumers: Contrasting the patterns and impacts of fire and mammalian herbivory in Africa. Philos Trans R Soc B Biol Sci. https://doi.org/10.1098/rstb.2015.0309
Starke Ayres (2023) Starke Ayres: Nutrifeed. https://www.starkeayres.com/products/home-gardening-seed/fertiliser/nutrifeed
Azad S, Sumon MH (2016) Species Specific Responses to Age on Nodule Formation, Seedling Growth, and Biomass Production of Acacia auriculiformis at Nursery Stage. J Bot. https://doi.org/10.1155/2016/6960783
Azad S, Sumon MMH (2017) Species specific nodulation responses and biomass accumulation to seedling age of Dalbergia sissoo at nursery stage. Rhizosphere 3:132–137. https://doi.org/10.1016/j.rhisph.2017.04.003
Azad S, Mondol S, Matin MA (2013) Functional relationships of nodulation response and biomass production at nursery stages of two fast-growing, leguminousmultipurpose tree species in Bangladesh: Albizia saman and Leucaena leucocephala. For Sci Pract 15:274–285. https://doi.org/10.1007/s11632-013-0416-2
Barnes ME (2001) Effects of large herbivores and fire on the regeneration of Acacia erioloba woodlands in Chobe National Park, Botswana. Afr J Ecol 39:340–350. https://doi.org/10.1046/j.1365-2028.2001.00325.x
Barnes RD, Fagg CW, Milton SJ (1997) Acacia erioloba: monograph and annotated bibliography. Trop For Pap ix
Bates D, Maechler M, Bolker B, Walker S (2015) Fitting Linear Mixed-Effects Models Using lme4. J Stat Softw 67:1–48. https://doi.org/10.18637/jss.v067.i01
Bivand R, Nicholas L-K (2019) Maptools: Tools for Handling Spatial Objects. R package version 0.9-9. https://cran.r-project.org/package=maptools
Bond WJ (2008) What Limits Trees in C 4 Grasslands and Savannas? Annu Rev Ecol Evol Syst 39:641–659. https://doi.org/10.1146/annurev.ecolsys.39.110707.173411
Bond WJ, Midgley JJ (2003) The Evolutionary Ecology of Sprouting in Woody Plants. Int J Plant Sci 164: 103–114.
Bouchenak-Khelladi Y, Maurin O, Hurter J, van der Bank M (2010) The evolutionary history and biogeography of Mimosoideae (Leguminosae): An emphasis on African acacias. Mol Phylogenet Evol 57:495–508. https://doi.org/10.1016/j.ympev.2010.07.019
Bunney K (2014) Seed Dispersal in South African Trees: with a focus on the megafaunal fruit and their dispersal agents. University of Cape Town
Case MF, Wigley BJ, Wigley-Coetsee C, Carla Staver A (2020) Could drought constrain woody encroachers in savannas? African J Range Forage Sci 37:19–29. https://doi.org/10.2989/10220119.2019.1697363
Chaulagain D, Frugoli J (2021) The regulation of nodule number in legumes is a balance of three signal transduction pathways. Int J Mol Sci 22:1–14. https://doi.org/10.3390/ijms22031117
Cramer MD, Chimphango SBM, Van Cauter A, Waldram MS, Bond WJ (2007) Grass competition induces N2 fixation in some species of African Acacia. J Ecol 95:1123–1133. https://doi.org/10.1111/j.1365-2745.2007.01285.x
Cramer MD, Van Cauter A, Bond WJ (2010) Growth of N2-fixing African savanna Acacia species is constrained by below-ground competition with grass. J Ecol 98:156–167. https://doi.org/10.1111/j.1365-2745.2009.01594.x
Cramer MD, Wakeling JL, Bond WJ (2012) Belowground competitive suppression of seedling growth by grass in an African savanna. Plant Ecol 213:1655–1666. https://doi.org/10.1007/s11258-012-0120-7
Deans JD, Ali OM, Lindley DK (1993) Rhizobial nodualtion of Acacia tree species in Sudan: soil inoculum potential and effects of peat. J Trop for Sci 6:56–64
Devine AP, McDonald RA, Quaife T, Maclean IMD (2017) Determinants of woody encroachment and cover in African savannas. Oecologia 183:939–951
Dovrat G, Sheffer E (2019) Symbiotic dinitrogen fixation is seasonal and strongly regulated in water-limited environments. New Phytol 221:1866–1877. https://doi.org/10.1111/nph.15526
Fall D, Ourarhi M, El Idrissi MM, Bakhoum N, Zoubeirou AM, Abdelmoumen H, Diouf D (2011) The efficiency and competitiveness of three Mesorhizobium sp. strains nodulating Acacia senegal (L.) Willd. under water deficiency conditions in the greenhouse. Symbiosis 54:87–94. https://doi.org/10.1007/s13199-011-0128-0
February EC, Higgins SI, Bond WJ, Swemmer L (2013) Influence of competition and rainfall manipulation on the growth responses of savanna trees and grasses. Ecology 94:1155–1164. https://doi.org/10.1890/12-0540.1
Fox J, Weisberg S (2019) An {R} Companion to Applied Regression, Third Edition. Thousand Oaks CA
Foxx AJ, Fort F (2019) Root and shoot competition lead to contrasting competitive outcomes under water stress: a systematic review and meta-analysis. PLoS ONE 14:1–17. https://doi.org/10.1371/journal.pone.0220674
Gavrin A, Kulikova O, Bisseling T, Fedorova EE (2017) Interface symbiotic membrane formation in root nodules of Medicago truncatula: the role of synaptotagmins MtSyt1, MtSyt2 and MtSyt3. Front Plant Sci 8:1–10. https://doi.org/10.3389/fpls.2017.00201
GBIF.org (9th April 2020a) GBIF Occurrence Download https://doi.org/10.15468/dl.dgxkj3
GBIF.org (9th April 2020b) GBIF Occurrence Download https://doi.org/10.15468/dl.y7btbk
Graves S, Piepho H-P, Selzer L, Dorai-Raj S (2019) multcompView: Visualizations of Paired Comparisons.
Gwata ET, Wofford DS, Pfahler PL, Boote KJ (2004) Genetics of promiscuous nodulation in soybean: nodule dry weight and leaf color score. J Hered 95:154–157. https://doi.org/10.1093/jhered/esh017
Hause B, Schaarschmidt S (2009) The role of jasmonates in mutualistic symbioses between plants and soil-born microorganisms. Phytochemistry 70:1589–1599. https://doi.org/10.1016/j.phytochem.2009.07.003
Hauwanga WN, McBenedict B, Strohbach BJ (2018) Trends of phanerophyte encroacher species along an aridity gradient on Kalahari sands, central Namibia. Eur J Ecol 4:41–47. https://doi.org/10.2478/eje-2018-0011
Higgins SI, Bond WJ, February EC, Bronn A, Euston-Brown DIW, Enslin B, Govender N, Rademan L, O’Regan S, Potgieter ALF, Scheiter S, Sowry R, Trollope L, Trollope WS (2007) Effects of four decades of fire manipulation on woody vegetation structure in savanna. Ecology 88:1119–1125. https://doi.org/10.1890/06-1664
Hijmans RJ (2020) raster: Geographic Data Analysis and Modeling. R package version 3.0–12.
Honda EA, Durigan G (2016) Woody encroachment and its consequences on hydrological processes in the savannah. Philos Trans R Soc B Biol Sci. https://doi.org/10.1098/rstb.2015.0313
House JI, Archer SR, Breshears DD, Scholes RJ, Tree N, Interactions G, Max P (2003) Conundrums in mixed woody – herbaceous plant systems. J Biogr 30(11):1763–1777
IPCC (2022) Climate Change 2022: Impacts Cambridge Adaptation and Vulnerability. Cambridge University Press
Kambatuku JR, Cramer MD, Ward D (2013) Nitrogen fertilisation reduces grass-induced N2 fixation of tree seedlings from semi-arid savannas. Plant Soil 365:307–320. https://doi.org/10.1007/s11104-012-1389-y
Kraaij T, Ward D (2006) Effects of rain, nitrogen, fire and grazing on tree recruitment and early survival in bush-encroached savanna, South Africa. Plant Ecol 186:235–246. https://doi.org/10.1007/s11258-006-9125-4
Kyalangalilwa B, Boatwright JS, Daru BH, Maurin O, van der Bank M (2013) Phylogenetic position and revised classification of Acacia s.l. (Fabaceae: Mimosoideae) in Africa, including new combinations in Vachellia and Senegalia. Bot J Linn Soc 172: 500–523. https://doi.org/10.1111/boj.12047
Lehmann CER, Parr CL (2016) Tropical grassy biomes: linking ecology, human use and conservation. Philos Trans R Soc B Biol Sci. https://doi.org/10.1098/rstb.2016.0329
Leitner M, Davies AB, Parr CL, Eggleton P, Robertson MP (2018) Woody encroachment slows decomposition and termite activity in an African savanna. Glob Chang Biol 24:2597–2606. https://doi.org/10.1111/gcb.14118
Lewis JR, Verboom GA, February EC (2021) Coexistence and bush encroachment in African savannas: the role of the regeneration niche. Funct Ecol 35:764–773. https://doi.org/10.1111/1365-2435.13759
Liu F, Archer SR, Gelwick F, Bai E, Boutton TW, Ben WuX (2013) Woody plant encroachment into grasslands: Spatial patterns of functional group distribution and community development. PLoS ONE 8:1–13. https://doi.org/10.1371/journal.pone.0084364
Ludwig F, Kroon H, Prins HHT, Berendse F (2001) Effects of nutrients and shade on tree-grass interactions in an East African savanna. J Veg Sci 12:579–588. https://doi.org/10.2307/3237009
Mantovani D, Veste M, Boldt-Burisch K, Fritsch S, Koning LA, Freese D (2015) Carbon allocation, nodulation, and biological nitrogen fixation of black locust (Robinia pseudoacacia L.) under soil water limitation. Ann for Res 58:259–274. https://doi.org/10.15287/afr.2015.420
Menge DNL, Wolf AA, Funk JL (2015) Diversity of nitrogen fixation strategies in Mediterranean legumes. Nat Plants 1(6):1–5
Moura J, Vieira EA (2020) Responses of young plants of Vachellia farnesiana to drought. Aust J Bot 68:587–594. https://doi.org/10.1071/BT20043
Mugunga CP, Sahinkuye D (2020) Assessment of seed germination and seedling growth of Vachellia sieberiana Under different soil moisture regimes. Rwanda J Agric Sci 2:4–14
Osborne CP, Charles-Dominique T, Stevens N, Bond WJ, Midgley G, Lehmann CER (2018) Human impacts in African savannas are mediated by plant functional traits. New Phytol 220:10–24
Parr CL, Gray EF, Bond WJ (2012) Cascading biodiversity and functional consequences of a global change-induced biome switch. Divers Distrib 18:493–503. https://doi.org/10.1111/j.1472-4642.2012.00882.x
Pellegrini AFA, Staver AC, Hedin LO, Charles-Dominique T, Tourgee A (2016) Aridity, not fire, favors nitrogen-fixing plants across tropical savanna and forest biomes. Ecology 97:2177–2183. https://doi.org/10.1002/ecy.1504
R Core Team (2020) R: A language and environment for statistical computing. R Foundation for Statistical Computing.
Russell JM, Tedder MJ, Demmer S (2019) Vachellia sieberiana var. woodii, a high-altitude encroacher: the effect of fire, frost, simulated grazing and altitude in north-western KwaZulu-Natal, South Africa. African J Range Forage Sci 36:169–180. https://doi.org/10.2989/10220119.2019.1667437
Sankaran M (2019) Droughts and the ecological future of tropical savanna vegetation. J Ecol 107:1531–1549. https://doi.org/10.1111/1365-2745.13195
Sankaran M, Ratnam J, Hanan NP (2004) Tree-grass coexistence in savannas revisited—Insights from an examination of assumptions and mechanisms invoked in existing models. Ecol Lett 7:480–490. https://doi.org/10.1111/j.1461-0248.2004.00596.x
Sankaran M, Hanan NP, Scholes RJ, Ratnam J, Augustine DJ, Cade BS, Gignoux J, Higgins SI, Le Roux X, Ludwig F, Ardo J, Banyikwa F, Bronn A, Bucini G, Caylor KK, Coughenour MB, Diouf A, Ekaya W, Feral CJ, February EC, Frost PGH, Hiernaux P, Hrabar H, Metzger KL, Prins HHT, Ringrose S, Sea W, Tews J, Worden J, Zambatis N (2005) Determinants of woody cover in African savannas. Nature 438:846–849. https://doi.org/10.1038/nature04070
Scholes RJ, Archer SR (1997) Tree-grass interactions in savannas. Annu Rev Ecol Syst 28:517–544
Senthilkumar M, Amaresan N, Sankaranarayanan A (2021) Plant-Microbe Interactions Laboratory Techniques
Serraj R (2003) Effects of drought stress on legume symbiotic nitrogen fixation: physiological mechanisms. Indian J Exp Biol 41:1136–1141
Seymour C (2003) Slowly does it: acacia erioloba growing large in Southern Kalahari Savannas. Glob Chang Biol 1:175–182
Seymour C (2008) Grass, rainfall and herbivores as determinants of Acacia erioloba (Meyer) recruitment in an African savanna. Plant Ecol 197:131–138. https://doi.org/10.1007/s11258-007-9366-x
Shetta ND (2015) Influence of drought stress on growth and nodulation of acacia origena (hunde) inoculated with indigenous rhizobium isolated from Saudi Arabia. J Agric Environ Sci 15:699–706. https://doi.org/10.5829/idosi.aejaes.2015.15.5.12629
Sprent JI (1995) Legume trees and shrubs in the tropics: N2 fixation in perspective. Soil Biol Biochem 27:401–407. https://doi.org/10.1016/0038-0717(95)98610-Z
Sprent JI (2009) Legume Nodulation: A Global Perspective. Wiley-Blackwell, Oxford
Sprent JI, Gehlot HS (2010) Nodulated legumes in arid and semi-arid environments: are they important? Plant Ecol Divers 3:211–219. https://doi.org/10.1080/17550874.2010.538740
Sprent JI, Odee DW, Dakora FD (2010) African legumes: a vital but under-utilized resource. J Exp Bot 61:1257–1265. https://doi.org/10.1093/jxb/erp342
Stevens N, Seal CE, Archibald S, Bond WJ (2014) Increasing temperatures can improve seedling establishment in arid-adapted savanna trees. Oecologia 175:1029–1040. https://doi.org/10.1007/s00442-014-2958-y
Stevens N, Lehmann CER, Murphy BP, Durigan G (2017) Savanna woody encroachment is widespread across three continents. Glob Chang Biol 23:235–244. https://doi.org/10.1111/gcb.13409
Sun J, Cardoza V, Mitchell DM, Bright L, Oldroyd G, Harris JM (2006) Crosstalk between jasmonic acid, ethylene and Nod factor signaling allows integration of diverse inputs for regulation of nodulation. Plant J 46:961–970. https://doi.org/10.1111/j.1365-313X.2006.02751.x
Sunmonu TO, Van Staden J (2014) Phytotoxicity evaluation of six fast-growing tree species in South Africa. South African J Bot 90:101–106. https://doi.org/10.1016/j.sajb.2013.10.010
Swaine EK, Swaine MD, Killham K (2007) Effects of drought on isolates of Bradyrhizobium elkanii cultured from Albizia adianthifolia seedlings of different provenances. Agrofor Syst 69:135–145. https://doi.org/10.1007/s10457-006-9025-6
Tadesse W, Desalegn G, Alia R (2007) Natural gum and resin bearing species of Ethiopia and their potential applications. Forest Syst 16:211. https://doi.org/10.5424/srf/2007163-01010
Tolk JA (2003) Plant available soil water. Encycl Water Sci. https://doi.org/10.1081/E-EWS
van der Merwe H, van Rooyen N, Bezuidenhout H, Bothma J, Du P, van Rooyen MW (2019) Vachellia erioloba dynamics over 38 years in the Kalahari Gemsbok national park, South Africa. Koedoe 61:1–12. https://doi.org/10.4102/koedoe.v61i1.1534
Van Der Merwe H, Van Rooyen N, Bezuidenhout H, Du J, Van Rooyen MW (2020) Woody vegetation change over more than 30 years in the interior duneveld of the Kalahari Gemsbok National Park. Bothalia 50:1–9. https://doi.org/10.38201/10.38201/BTHA.ABC.V50.I1.2
Veldhuis MP, Hulshof A, Fokkema W, Berg MP, Olff H (2016) Understanding nutrient dynamics in an African savanna: local biotic interactions outweigh a major regional rainfall gradient. J Ecol 104:913–923. https://doi.org/10.1111/1365-2745.12569
Venter ZS, Cramer MD, Hawkins HJ (2018) Drivers of woody plant encroachment over Africa. Nat Commun 9:1–7. https://doi.org/10.1038/s41467-018-04616-8
Vicca S, Gilgen AK, Camino Serrano M, Dreesen FE, Dukes JS, Estiarte M, Gray SB, Guidolotti G, Hoeppner SS, Leakey ADB, Ogaya R, Ort DR, Ostrogovic MZ, Rambal S, Sardans J, Schmitt M, Siebers M, van der Linden L, van Straaten O, Granier A (2012) Urgent need for a common metric to make precipitation manipulation experiments comparable. New Phytol 195:518–522. https://doi.org/10.1111/j.1469-8137.2012.04224.x
Vieira EA, Andrade Galvão FC, Barros AL (2019) Influence of water limitation on the competitive interaction between two Cerrado species and the invasive grass Brachiaria brizantha cv. Piatã Plant Physiol Biochem 135:206–214. https://doi.org/10.1016/j.plaphy.2018.12.002
Voisin AS, Salon C, Jeudy C, Warembourg FR (2003) Root and nodule growth in Pisum sativum L. in relation to photosynthesis: analysis using 13C-labelling. Ann Bot 92:557–563. https://doi.org/10.1093/aob/mcg174
Wickham H (2016) ggplot2: Elegant Graphics for Data Analysis
Winters G, Otieno D, Cohen S, Bogner C, Ragowloski G, Paudel I, Klein T (2018) Tree growth and water-use in hyper-arid Acacia occurs during the hottest and driest season. Oecologia 188:695–705. https://doi.org/10.1007/s00442-018-4250-z
Wurzburger N, Miniat CF (2014) Drought enhances symbiotic dinitrogen fixation and competitive ability of a temperate forest tree. Oecologia 174:1117–1126. https://doi.org/10.1007/s00442-013-2851-0
Zahran HH (1999) Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiol Mol Biol Rev 63:968–989
Zheng M, Zhou Z, Zhao P, Luo Y, Ye Q, Zhang K, Song L, Mo J (2020) Effects of human disturbance activities and environmental change factors on terrestrial nitrogen fixation. Glob Chang Biol 26:6203–6217. https://doi.org/10.1111/gcb.15328
Zinn AD, Ward D, Kirkman K (2007) Inducible defences in Acacia sieberiana in response to giraffe browsing. African J Range Forage Sci 24:123–129. https://doi.org/10.2989/AJRFS.2007.24.3.2.295
Acknowledgements
Thanks to Dr Marius Rossouw for logistic support and Dr Kyle Dexter for statistical advice. EMT was supported by a NERC Doctoral Training Partnership grant (NE/S007407/1), and a grant from the University of Edinburgh Moray Endowment Fund Award. CERL was supported by an International Collaboration Award from the Royal Society (IC17005).
Funding
This work was supported by a NERC Doctoral Training Partnership grant (NE/S007407/1), a grant from the University of Edinburgh Moray Endowment Fund Award was awarded to EMT and an International Collaboration Award to CERL (IC17005).
Author information
Authors and Affiliations
Contributions
Conceptualization: all authors. Data collection: EMT, NS. Statistical analyses: EMT, NS, CERL, Writing: all authors.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there is no conflict of interest.
Ethical approval
This study was approved by University of Edinburgh School of GeoScience Research Ethics & Integrity Committee.
Additional information
Communicated by Jesse Kalwij.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Telford, E.M., Stevens, N., Midgley, G.F. et al. Nodulation alleviates the stress of lower water availability in Vachellia sieberiana. Plant Ecol 224, 387–402 (2023). https://doi.org/10.1007/s11258-023-01302-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11258-023-01302-8