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Interaction of nitrogen availability in the soil with leaf physiological traits and nodule formation of Robinia pseudoacacia-rhizobia symbiosis depends on provenance

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Abstract

Aims

The interaction between nitrogen (N) availability in the soil, rhizobia nodule formation and leaf physiological traits of Robinia pseudoacacia L. was explored at initial nodule development.

Methods

We selected two Robinia provenances, one from Northwest (GS) and one from Northeast China (DB), and cultivated seedlings in the greenhouse with and without rhizobia inoculation at normal and high N supply in the soil. After ca. 2.5 months growth, nodule formation, plant biomass, CO2 and H2O gas exchange of the leaves, and foliar N contents and partitioning were analyzed.

Results

Rhizobia inoculation strongly promoted the formation of root nodules independent of N availability in the soil, but this effect was more pronounced in the DB than for GS provenance. It reduced biomass accumulation of the GS provenance, but not for DB provenance at both, normal and high soil N availability. High N supply did not affect biomass accumulation independent of rhizobia inoculation. Leaf photosynthesis of both Robinia origins was enhanced by high N supply, but this effect was counteracted by rhizobia inoculation only in leaves of DB plants. In GS but not in DB plants, high N supply reduced not only nodule formation, but also stomatal conductance, but still enhanced transpiration without modifying the foliar water content. In addition, high N supply plus inoculation enhanced the organic N content in GS plants rather than DB plants.

Conclusion

These results indicate that excess N availability in the soil interacts with the performance of Robinia provenances, as previously reported for drought and phosphorus (P) depletion.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors extend them thanks to the graduate-fellow team of Center of Molecular Ecophysiology (CMEP), College of Resources and Environment, Southwest University for their excellent assistance in the greenhouse planting and sampling work. The financial supports of the “Double-First Class” Initiative Program for Foreign Talents of Southwest University and the “Prominent Scientist Program” of Chongqing Talents (cstc2021ycjh-bgzxm0002 & cstc2021ycjh-bgzxm0020), China are gratefully acknowledged.

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  1. Hongguang Sun and Bin Hu contributed equally to this work.

Authors and Affiliations

  1. Center of Molecular Ecophysiology (CMEP), College of Resources and Environment, Southwest University, No. 2 Tiansheng Road, Beibei District, 400715, Chongqing, People’s Republic of China

    Hongguang Sun, Rui Liu, Hui Yuan, Mi Zhou, Zhenshan Liu, Bin Hu & Heinz Rennenberg

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  1. Hongguang Sun
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  2. Rui Liu
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  3. Hui Yuan
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  6. Bin Hu
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  7. Heinz Rennenberg
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Hongguang Sun, Rui Liu, Zhenshan Liu, Mi Zhou and Hui Yuan. The first draft of the manuscript was written by Hongguang Sun and Bin Hu. Heinz Rennenberg and Bin Hu contributed to the finalization and revision of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bin Hu.

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Sun, H., Liu, R., Yuan, H. et al. Interaction of nitrogen availability in the soil with leaf physiological traits and nodule formation of Robinia pseudoacacia-rhizobia symbiosis depends on provenance. Plant Soil 490, 239–259 (2023). https://doi.org/10.1007/s11104-023-06069-5

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