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Molecular and functional characterization of the HbSRPP promoter in response to hormones and abiotic stresses

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Abstract

Small rubber particle protein (SRPP) is a major component of Hevea brasiliensis latex, and obviously participates in the biosynthesis of natural rubber. However, little information is available about regulation of SRPP gene expression. In this study, the promoter region of HbSRPP was isolated and characterized. Its sequence included regulatory elements predicted to be responsive to hormones and other environmental cues. Promoter deletion analysis revealed that 219 nucleotides (nt) upstream of the transcription start site were sufficient for expression. The region from −1,055 to −219 nt positively regulated expression induced by methyl jasmonate (MeJA), abscisic acid (ABA), and wounding; the region from −734 to −528 nt positively regulated expression induced by gibberellic acid (GA); the region from −734 to −219 nt positively regulated expression induced by heat; the region from −1,055 to −4 negatively regulated expression induced by cold; the region from −219 to −4 nt was associated with negative regulation of expression induced by ABA and wounding; the region from −528 to −4 nt negatively regulated expression induced by GA. These results suggest the activity of the HbSRPP promoter is regulated by MeJA, ABA, GA, cold, heat, and wounding.

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Abbreviations

ABA:

Abscisic acid

GA:

Gibberellic acid

GUS:

β-Glucuronidase

MeJA:

Methyl jasmonate

NR:

Natural rubber

nt:

Nucleotides

SRPP:

Small rubber particle protein

TSS:

Transcription start site

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31170634, 30960160), the National Nonprofit Institute Research Grant of ITBB (110206), and the Major Technology Project of Hainan (ZDZX2013023-1).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Dong Guo, Hui-Liang Li, Xiao Tang & Shi-Qing Peng

  2. College of Agriculture, Hainan University, Haikou, 570228, China

    Xiao Tang & Shi-Qing Peng

Authors
  1. Dong Guo
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  2. Hui-Liang Li
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  3. Xiao Tang
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  4. Shi-Qing Peng
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Corresponding author

Correspondence to Shi-Qing Peng.

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11248_2013_9753_MOESM1_ESM.eps

The genome sequence and gene structure of the HbSRPP. a Nucleotide and deduced amino acid sequences of HbSRPP. b Structures of the HbSRPP gene. Exons are indicated as blue boxes and introns as grew grids boxes (EPS 2623 kb)

11248_2013_9753_MOESM2_ESM.eps

Multiple sequence alignment of the cDNA 5′ end of HbSRPP transcripts. cDNA ends were amplified using 5′-RACE strategy. The ATG is black frame. A 104 bp distance upstream of ATG is the putative start of transcription (EPS 684 kb)

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Guo, D., Li, HL., Tang, X. et al. Molecular and functional characterization of the HbSRPP promoter in response to hormones and abiotic stresses. Transgenic Res 23, 331–340 (2014). https://doi.org/10.1007/s11248-013-9753-0

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  • DOI: https://doi.org/10.1007/s11248-013-9753-0

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