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Studies on the interactions of a novel ruthenium(II) complex with G-quadruplex DNA

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Abstract

A novel ruthenium(II) porphyrin complex, [Ru(phen)2MPyTPP] (phen = phenanthroline, MPyTPP = mono-(3′-Pyridine)-10,15,20-triphenyl porphyrin), has been prepared and characterized by elemental analysis, ESI-MS and NMR methods. The interaction of the complex with G-quadruplex DNA has been studied by spectroscopy, and the results have shown that [Ru(phen)2MPyTPP] can bind to G-quadruplex d(TTAGGG)4 with high affinity. In the presence of d(TTAGGG)4, the characteristic Soret and intra-ligand (IL) transitions in the UV–Vis spectrum of the complex exhibit hypochromism of 25% (Δλ = 6 nm) and 18% (Δλ = 1 nm), respectively. The intrinsic binding constant was calculated according to the decay of the Soret band as 3.02(±0.08) × 106 M−1. The positive signal of d(TTAGGG)4 in its circular dichroism spectra was clearly decreased in the presence of the complex, indicating the conformation of the G-quadruplex was severely disturbed by the ruthenium(II) complex.

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Acknowledgment

We are grateful to the Nature Science Foundation of Guangdong Province (04300624) and the Technology Foundation of Guangdong Province (2007B031513004) for their financial supports.

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Authors and Affiliations

  1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Wen-Jie Mei, Xin-Yu Wei & Yun-Jun Liu

  2. YanJing Medical College, Capital Medical University, Peking, 101300, China

    Bei Wang

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  1. Wen-Jie Mei
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  2. Xin-Yu Wei
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  3. Yun-Jun Liu
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  4. Bei Wang
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Correspondence to Wen-Jie Mei or Bei Wang.

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Mei, WJ., Wei, XY., Liu, YJ. et al. Studies on the interactions of a novel ruthenium(II) complex with G-quadruplex DNA. Transition Met Chem 33, 907–910 (2008). https://doi.org/10.1007/s11243-008-9131-6

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