Abstract
The relationship between different types of explants and the content of endogenous hormones during somatic embryogenesis was studied by high-performance liquid chromatography-mass spectrometry (HPLC-MS) in Cinnamomum camphora, providing a theoretical basis for understanding the mechanism underlying the development of somatic embryos in the process of somatic embryogenesis. Embryogenic callus (EC), non-embryogenic callus (NEC), and somatic embryos (SE) were used as research materials to measure and analyze the activity of metabolic-related enzymes. The results showed that the activity of superoxide dismutase (SOD) and peroxidase (POD) reached the highest in the EC and SE, which could promote and induce the development of embryonic cells. Lower activity of malondialdehyde (MDA) was observed in EC and SE, indicating that they had stronger stress resistance. Lower levels of catalase (CAT) metabolism might promote differentiation of somatic embryos. The contents of indole-3-acetic acid (IAA) and abscisic acid (ABA) were higher in all three types of materials, and the ratios of ABA/IAA, ABA/gibberellins (GA3), and IAA/zeatin (ZT) reached the highest values in SE followed by EC, indicating that higher ratios of ABA/IAA, ABA/GA3, and IAA/ZT were beneficial to somatic embryogenesis.
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References
Aebi H (1984) Catalase in vitro. Method Enzymol 105:121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Alsher RG, Erturk N, Heath L (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341. https://doi.org/10.1093/jexbot/53.372.1331
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Blazquez S, Olmos E, Hernández JA, Fernández-García N, Fernández JA, Piqueras A (2009) Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system. Plant Cell Tiss Organ Cult 97:49–57. https://doi.org/10.1007/s11240-009-9497-y
Centeno ML, Rodríguez R, Berros B, Rodríguez A (1997) Endogenous hormonal content and somatic embryogenic capacity of Corylus avellana L. cotyledons. Plant Cell Rep 17:139–144. https://doi.org/10.1007/s002990050367
Dai XY, Liu XL, Zhang T, Jiang L, Cheng Z (2019) Embryogenic callus induction and somatic embryogenesis from immature zygotic embryos of Cinnamomum Camphor. Acta Agriculturae Universitatis Jiangxiensis 41:1120–1129. https://doi.org/10.13836/j.jjau.2019131
Du L (2014) Research on cotyledonary embryo culture of Cinnamomum camphora L. North Horticult 38:89–92 (In Chinese)
Du L, Ye YM, Bao MZ (2006) Study on somatic embryogenesis and plant regeneration of immature zygotic embryo of Cinnamomum camphora. Sc Silv Sin 42:37–39. https://doi.org/10.11707/j.1001-7488.20060607
Du L, Li YP, Yao Y, Zhang LW (2015) An efficient protocol for plantlet regeneration via direct organogenesis by using nodal segments from embryo-cultured seedlings of Cinnamomum camphora L. PLoS ONE 10(5):e0127215. https://doi.org/10.1371/journal.pone.0127215
Fatima S, Mujib A, Samaj J (2011) Anti-oxidant enzyme responses during in vitro embryogenesis in Catharanthus roseus. J Hortic Sci Biotech 86:569–574. https://doi.org/10.1080/14620316.2011.11512805
Find J, Grace L, Krogstrup P (2002) Effect of anti-auxins on maturation of embryogenic tissue cultures of Nordmanns fir (Abies Nordmanniana). Physiol Plant 116:231–237. https://doi.org/10.1034/j.1399-3054.2002.1160213.x
Grzyb M, Kalandyk A, Waligórski P, Mikuła A (2017) The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb. Plant Cell Tiss Organ Cult 129:387–397. https://doi.org/10.1007/s11240-017-1185-8
Guo HR, Li JM, Ma Y, Zhu ZF, Du L (2022) Somatic embryogenesis processes and changes in endogenous hormone content of Cinnamomum camphora L. Res Square. https://doi.org/10.21203/rs.3.rs-2293102/v1
He YQ, Wang JJ (2015) Tissue culture and plant regeneration of Cinnamomum camphora Yongjin. J South Agric 46:96–100 (In Chinese)
Inger H, Henrik H, Joakim P (2009) The polar auxin transport inhibitor NPA impairs embryo morphology and increases the expression of an auxin efflux facilitator protein PIN during Picea abies somatic embryo development. Tree Physiol 29:483–496. https://doi.org/10.1093/treephys/tpn048
Jiang CR, Peng FR, Tan PP (2014) Somatic embryogenesis and the physiological and biochemical characteristics in Catalpa fargesii Bur. f. duclouxii (Dode) Gilmour. J for Eng 28:25–29. https://doi.org/10.13360/j.issn.1000-8101.2014.01.006
Jiménez VM (2005) Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul 47:91–110. https://doi.org/10.1007/s10725-005-3478-x
Jiménez VM, Bangerth F (2000) Relationship between endogenous hormone levels of grapevine callus cultures and their morphogenetic behaviour. Vitis 39:151–157. https://doi.org/10.5073/vitis.2000.39.151-157
Jiménez VM, Bangerth F (2001) Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot. Physiol Plant 111:389–395. https://doi.org/10.1034/j.1399-3054.2001.1110317.x
Jiménez VM, Guevara E, Herrera J, Bangerth F (2005) Evolution of endogenous hormone concentration in embryogenic cultures of carrot during early expression of somatic embryogenesis. Plant Cell Rep 23:567–572. https://doi.org/10.1007/s00299-004-0869-9
Jing RY, Wang PL, Huang Z, Li ZH (2019) Histocytological study of somatic embryogenesis in the tree Cinnamomum camphora L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47:1348–1358. https://doi.org/10.15835/nbha47411655. Lauraceae
Jing RY, Huo K, Li ZH (2020) Difference between embryogenic and non-embryogenic callus of Cinnamomum camphora L. J Cent South Univ T 40:70–78. https://doi.org/10.14067/j.cnki.1673-923x.2020.10.008
Kim DH, Kang KW, Sivanesan I (2019) Influence of auxins on somatic embryogenesis in Haworthia Retusa Duval. Biologia 74:25–33. https://doi.org/10.2478/s11756-018-0151-1
Li S, Zhang QQ, Zhang HM, Wang J, Sun JJ, Yang XR, Huang SW, Zhang ZH (2022) Deletion of a cyclin-dependent protein kinase inhibitor, CsSMR1, leads to dwarf and determinate growth in cucumber (Cucumis sativus L). Theor Appl Genet 135:915–927
Liang Y, Shen HL, Gao ML (2016) Content dynamics of endogenous hormones in different seed developmental stages of Korean Pine. Sc Silv Sin 52:105–111. https://doi.org/10.11707/j.1001-7488.20160213
Liang Y, Xu X, Shen HL, Gao ML, Zhao Y, Bai X (2022) Morphological and endogenous phytohormone changes during long-term embryogenic cultures in Korean pine. Plant Cell Tiss Organ Cult 151: 253–264. https://doi.org/10.1007/s11240-022-02348-8
Liao YK, Liao CK, Ho YL (2008) Maturation of somatic embryos in two embryogenic cultures of Picea Morrisonicola Hayata as affected by alternation of endogenous IAA content. Plant Cell Tiss Organ Cult 93:257–268. https://doi.org/10.1007/s11240-008-9371-3
Libik M, Konieczny R, Pater B, Ślesak I, Miszalski Z (2005) Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Rep 23:834–841. https://doi.org/10.1007/s00299-004-0886-8
Manivannan A, Jana S, Soundararajan P, Ko CH, Jeong BR (2015) Antioxidant enzymes metabolism and cellular differentiation during the developmental stages of somatic embryogenesis in Torilis japonica (Houtt.) DC. Plant Omics 8:461–471. https://doi.org/10.3316/informit.516864120146941
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.:473– 97
Nakagawa H, Saijyo T, Yamauchi N, Shigyo M, Kako S, Ito A (2001) Effects of sugars and abscisic acid on somatic embryogenesis from melon (Cucumis melo L.) expanded cotyledon. Sci Hortic-Amsterdam 90:85–92. https://doi.org/10.1016/S0304-4238(00)00259-4
Nic-Can GI, Loyola-Vargas VM (2016) The role of the auxins during somatic embryogenesis. In: Loyola-Vargas VM (ed) In somatic embryogenesis: fundamental aspects and applications. Springer, Switzerland, pp 171–182. https://doi.org/10.1007/978-3-319-33705-0_10
Pérez JM, Cantero NE, Pérez AF, Le DI, Guivarc’h A, Cos TJ (2014) Relationship between endogenous hormonal content and somatic organogenesis in callus of peach (Prunus persica L. Batsch) cultivars and Prunus persica × Prunus dulcis rootstocks. J Plant Physiol 171:619–624. https://doi.org/10.1016/j.jplph.2014.01.006
Schmedes A, Hølmer G (1989) A new thiobarbituric acid (TBA) method for determining free malondialdehyde (MDA) and hydroperoxides selectively as a measure of lipid peroxidation. J Am Oil Chem Soc 66:813–817. https://doi.org/10.1007/BF02653674
Shi XP, Dai XG, Liu GF, Zhang JW, Ning GG, Bao MZ (2010) Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L). In Vitro Cell Dev Biol-Plant 46:117–125. https://doi.org/10.1007/s11627-009-9272-0
Statements & Declarations
Su YH, Su YX, Liu YG, Zhang XS (2013) Abscisic acid is required for somatic embryo initiation through mediating spatial auxin response in Arabidopsis. Plant Growth Regul 69:167–176. https://doi.org/10.1007/s10725-012-9759-2
Tian DL, Luo Y, Xiang WH, Yan WD (2004) Photosynthetic characteristics of Cinnamomum camphora and its response to elevation of CO2 and temperature. Sc Silv Sin 40:88–92. https://doi.org/10.11707/j.1001-7488.20040514
Türkan S, Bor M, Zdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. Acutifolius Gray and drought-sensitive P. Vulgaris L subjected to polyethylene glycol mediated water stress. Plant Sci 168:231. https://doi.org/10.1016/j.plantsci.2004.07.032
Wang LL, Zhou YL, Ding Y, Chen CR, Chen XT, Su NN, Zhang XG, Pan Y, Li JH (2023) Novel flavin-containing monooxygenase protein FMO1 interacts with CAT2 to negatively regulate drought tolerance through ROS homeostasis and ABA signaling pathway in tomato. Hortic Res 10(4):uhad037. https://doi.org/10.1093/hr/uhad03
Wu G, Wei XL, Wang X, Wei Y (2021) Changes in biochemistry and histochemical characteristics during somatic embryogenesis in Ormosia Henryi Prain. Plant Cell Tiss Organ Cult 144:505–517. https://doi.org/10.1007/s11240-020-01973-5
Xu CY, Ma YD, Tian ZF, Luo QY, Zheng TF, Wang B, Zuo ZJ (2022) Monoterpene emissions and their protection effects on adult Cinnamomum camphora against high temperature. Trees 36:711–721. https://doi.org/10.1007/s00468-021-02242-4
Zhou SY, Wang YQ (2017a). Relationship between embryonic callus induction from leaves and endogenous hormone content in loquat (Eriobotrya japonica Lindl.). Plant Sci J 35(1):99–106. (In Chinese)
Zhou Y, Yan W (2016) Conservation and applications of camphor tree (Cinnamomum camphora) in China: ethnobotany and genetic resources. Genet Resour Crop Ev 63:1049–1061. https://doi.org/10.1007/s10722-015-0300-0
Zhou XH, Zheng RH, Liu GX, Xu Y, Zhou YW, Laux T, Zhen Y, Harding S, Shi JS, Chen JH (2017b) Desiccation treatment and endogenous IAA levels are key factors influencing high frequency somatic embryogenesis in Cunninghamia lanceolata (Lamb.) Hook. Front Plant Sci 8:2054. https://doi.org/10.3389/fpls.2017.02054
Acknowledgements
The work was supported by grants from the Research Project from Key R&D Program and Promotion of Henan Province (Grant No. 202102110078) and Postgraduate Education Reform and Quality Improvement Project of Henan Province (Grant No. YJS2021JD17).
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Funding provided by Research Project from Key R&D Program and Promotion of Henan Province (Grant No. 202102110078). Postgraduate Education Reform and Quality Improvement Project of Henan Province (Grant No. YJS2021JD17).
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HG: Writing—original draft, Visualization; XK: Investigation, Methodology, Data curation; MY: Investigation, Validation; RW: Software, Methodology; LD Writing—review & editing, Funding acquisition.
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Guo, H., Kang, X., Yuan, M. et al. Relationship between somatic embryogenesis and endogenous hormones of Cinnamomum camphora L.. Plant Cell Tiss Organ Cult 156, 53 (2024). https://doi.org/10.1007/s11240-024-02682-z
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DOI: https://doi.org/10.1007/s11240-024-02682-z