Abstract
Rhizobium rhizogenes mediated genetic transformation is a promising biotechnological tool to obtain specialized metabolites having valuable biological activities. The present study reports a reliable transformation protocol for hairy root induction with sustainable growth and identification of specialized metabolites with antioxidant activities in hybrid poplar clone 717-1B4 (Populus tremula × Populus alba). Four different wild type agropine R. rhizogenes strains ATCC 15834, A4, MSU 440 and LBA 9402 were employed to check their efficiency on hairy root induction and accumulation of specialized metabolites. Results indicated that the transformation efficiency was highly dependent on strain used and could be increased by adding acetosyringone into the co-cultivation medium. PCR amplification of rolB and rolC genes confirmed the transgenic nature of the hairy roots. Adding polyvinylpyrrolidone into the culture media not only circumvented the problem of browning due to phenolics but also resulted in healthy and fast growth of hairy roots. Colorimetric assays revealed the presence of phenolic compounds, among which flavonoids in ethanolic extracts of different hairy root lines which could be associated with the significant antioxidant activities as depicted by FRAP and DPPH assays. The hairy root lines obtained with different R. rhizogenes strains showed similar phytochemical profiles. UHPLC-HRMS analysis allowed the separation of 136 peaks, out of which catechin, nigracin, salireposide, trichocarpin and tremulacin were identified. In conclusion, poplar hairy roots are a promising raw material for producing specialized metabolites with bioactive properties that could be further used for various pharmaceutical, food and cosmetics applications.
Key message
A sustainable hairy root culture system was developed on poplar model tree. A first UHPLC-MS phytochemical analysis of the root compartment is provided, revealing the presence of antioxidant bioactive compounds.
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Data availability
The data that support the findings of this study are available upon request from the corresponding author.
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Acknowledgements
The authors thank Prof. Aleksandra Królicka, University of Gdansk, Poland, for providing R. rhizogenes strains ATCC 15834, A4 (ATCC 31798) and LBA 9402 (NCPPB 1855) and Dr. Jose Manuel García Garrido, Zaidín Experimental Station (EEZ), CSIC, Granada, Spain for providing strain MSU 440.
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This work was funded by the Région Centre Val de Loire through the CosmetoPop research project (20037UNI0000A) as part of the ARD CVL Cosmetosciences 2020 program.
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Material preparation, data collection and analysis: MaL, MB, FC, CC, SH, MeL, MS-V, SM; Study conception and design: FH, DM, ED, SC, SM, FL; Writing—original draft preparation: MB, SH, ED, SM, SC, FL; Reviewing and editing: SM, FL; Funding acquisition and Project administration: SC, FL. All authors read and approved the final manuscript.
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Laffon, M., Bruat, M., Chefdor, F. et al. Hairy root induction in hybrid poplar (Populus tremula × Populus alba) for sustainable growth and specialized metabolites production with antioxidant activities. Plant Cell Tiss Organ Cult 156, 2 (2024). https://doi.org/10.1007/s11240-023-02634-z
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DOI: https://doi.org/10.1007/s11240-023-02634-z