Abstract
The moisture content is a key factor affecting the success of pollen cryopreservation. In this study, Paeonia lactiflora pollen with different moisture contents were used in determination of the pollen viability, reactive oxygen species (ROS), oxidative damage indices and antioxidants before and after cryopreservation, in order to investigate the effects of ROS-induced oxidative stress on pollen viability. The viability of pollen with high moisture content (9.9%) decreased significantly after cryopreservation, while the viability of pollen cryopreserved with low moisture content (6.33% and 3.15%) was not changed significantly. The hydrogen peroxide (H2O2), hydroxyl radical (·OH) and malondialdehyde (MDA), and glutamate reductase (GR) activities of pollen with higher moisture content were significantly increased after cryopreservation, while the ascorbic acid peroxidase (APX) activity and the ascorbic acid (AsA) and glutathione (GSH) content were not changed; however, in pollens with lower moisture content (6.33% and 3.15%), the ROS components, oxidative damage indices and antioxidant content were relatively stable after cryopreservation. In addition, correlation analysis showed that the viability levels of cryopreserved pollens were positively correlated with the content of superoxide anions (O2·−), CAT activity, APX activity, AsA and GSH, and negatively correlated with the content of H2O2 and activities of ·OH, MDA, SOD and GR. These results indicated the ROS contents of pollen following cryopreservation procedures was affected by its moisture content, and the increased ROS contents were the main reasons for decreased viability in pollens with high moisture content.
Key message
The oxidative stress induced by reactive oxygen species decreased the viability of Paeonia lactiflora pollen after cryopreservation, and was affected by pollen moisture content.
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Acknowledgements
This research was supported by the National Natural Science Foundation of China Youth Science Foundation Project (No. 32201613); Youth Scientific Research Project of Shanxi Basic Research Program (No. 202103021223170); Doctoral research project of Shanxi Agricultural University (No. 2021BQ108); Scientific research project for work in Shanxi of doctor (No. SXBYKY2022036).
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RR designed the research, completed the experiments, analyzed the data and drafted the manuscript. JG and ZJ completed the experiments. XY conceived the project, supervised the analysis and critically revised the manuscript. All authors read and approved the manuscript.
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Ren, R., Guo, J., Ji, Z. et al. ROS-induced oxidative stress affects the viability of cryopreserved Paeonia lactiflora pollen with different moisture contents. Plant Cell Tiss Organ Cult 155, 117–126 (2023). https://doi.org/10.1007/s11240-023-02558-8
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DOI: https://doi.org/10.1007/s11240-023-02558-8