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Increased antioxidant activity in Cassia seedlings under UV-B radiation

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Biologia Plantarum

Abstract

Cassia auriculata L. seedlings were irradiated with ultraviolet B (UV-B) in an environment-control chamber. The two doses assayed (7.5 and 15.0 kJ m−2) induced oxidative damage with an increase in lipid peroxidation and hydrogen peroxide and a decrease in chlorophyll and total phenol contents. The ascorbate and dehydroascorbate content as well as the reduced glutathione/oxidized glutathione content and ratio were significantly increased. The UV-B stress led to significant increases of the activity of superoxide dismutase, catalase, peroxidase and polyphenol oxidase. It is suggested that Cassia seedlings try to counteract high concentrations of oxygen species produced under UV-B stress through a co-ordinated increase in the contents and activities of antioxidants involved in their detoxification.

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Abbreviations

ASA:

ascorbate

CAT:

catalase

DHA:

dehydroascorbate

DTNB:

5,5′-dithio-bis-(2-nitrobenzoic acid)

EDTA:

ethylenediaminetetraacetic acid (disodium salt)

f.m.:

fresh mass

GSH:

reduced glutathione

GSSG:

oxidized glutathione

NADPH:

reduced nicotinamide adenine dinucleotide phosphate

NBT:

nitroblue tetrazolium

POX:

peroxidase

PPO:

polyphenol oxidase

PVP:

polyvinylpyrrolidone

ROS:

reactive oxygen substances

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TBARS:

thiobarbituric acid reactive substances

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  1. Stress Physiology Laboratory, Department of Botany, JNV University, Jodhpur, Rajasthan, 342011, India

    S. Agarwal

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Agarwal, S. Increased antioxidant activity in Cassia seedlings under UV-B radiation. Biol Plant 51, 157–160 (2007). https://doi.org/10.1007/s10535-007-0030-z

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  • DOI: https://doi.org/10.1007/s10535-007-0030-z

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