Abstract
In conventional in vitro culture, plantlets are kept in closed containers to avoid contamination and drying of the explant. Ventilation inside the containers improves plant growth and affects secondary metabolism, as it modifies the microenvironmental conditions. Therefore, the objective of this study was to evaluate the effect of the use of ventilation systems on the growth characteristics and production of photosynthetic pigments and volatile organic compounds (VOCs) in Aeollanthus suaveolens Mart. ex Spreng. (Lamiaceae) cultured in vitro. Nodal segments containing one pair of leaves were cultured in a conventional system (NMS) and in natural ventilation systems with one (AMS1), two (AMS2) and four (AMS4) porous membranes. At 40 days, the plantlets were evaluated for growth, VOC concentration, and photosynthetic pigment production. The number of porous membranes used in the vial cap affected growth, photosynthetic pigments and VOCs. A higher number of porous membranes (AMS4) led to greater dry weight accumulation, increased production of photosynthetic pigments, and enhanced synthesis of (Z)-β-farnesene. Lower growth and fewer photosynthetic pigments, and increase linalool acetate synthesis were observed in the culture without the use of porous membranes (NMS). The leaf area of plantlets cultivated with the use of four membranes was 3.8 times greater than that of plantlets cultivated without the use of membranes. For the photoautotrophic cultivation of A. suaveolens in vitro, the use of natural ventilation with four membranes is recommended because it promotes better growth, increases the production of photosynthetic pigments and is superior to the conventional sealed system.
Key Message
Alternative membrane systems led to differentiation in the contents of the main volatile organic compound and improve anatomical characteristics, accumulation of photosynthetic pigments and dry weight.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on a reasonable request.
Abbreviations
- VOCs:
-
Volatile organic compounds
- AMS1:
-
Alternative membrane system using lids with one membrane
- AMS2:
-
Alternative membrane system using lids with two membranes
- AMS4:
-
Alternative membrane system using lids with four membranes
- NMS:
-
No membrane system
- SN:
-
Shoot number
- LN:
-
Leaf number
- RN:
-
Root number
- SL:
-
Shoot length
- LLR:
-
Length of longest root
- TLA:
-
Total leaf area
- LDW:
-
Leaf dry weight
- SDW:
-
Stem dry weight
- RDW:
-
Root dry weight
- TDW:
-
Total dry weight
References
Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry, vol 456. Allured publishing corporation, Carol Stream
Alvarez C, Sáez P, Sáez K, Sánchez-Olate M, Ríos D (2012) Effects of light and ventilation on physiological parameters during in vitro acclimatization of Gevuina avellana. Plant Cell Tissue Organ Cult 110(1):93–101. https://doi.org/10.1007/s11240-012-0133-x
Arigita L, Cañal MJ, Tamés RS, González A (2010) CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson). In Vitro Cell. Dev Biol Plant 46(3):312–322. https://doi.org/10.1007/s11627-009-9267-x
Barreiro AP, Zucareli V, Ono EO, Rodrigues JD (2006) Growth analysis of basil plants submitted to plant growth regulators. Bragantia 65:563–567. https://doi.org/10.1590/S0006-87052006000400005
Benincasa MMP (2003) Plant growth analysis: basics. FUNEP, Jaboticabal
Chagas JH, Pinto JEB, Bertolucci SKV, Costa AG, de Jesus HCR, Alves PB (2013) Production, content and chemical composition of essential oil of mint cultivated under color shading nets. Hort Bras 31:297–303. https://doi.org/10.1590/S0102-05362013000200020
Chen C (2006) In situ measurement of microclimate for the plantlets cultured in vitro. Biosyst Eng 95(3):413–423. https://doi.org/10.1016/j.biosystemseng.2006.08.001
Costa AC, Rosa M, Megguer CA, Silva FG, Pereira FD, Otoni WC (2014a) A reliable methodology for assessing the in vitro photosynthetic competence of two Brazilian savanna species: Hyptis marrubioides and Hancornia speciosa. Plant Cell Tissue Organ Cult 117(3):443–454. https://doi.org/10.1007/s11240-014-0455-y
Costa AG, Chagas JH, Bertolucci SK, Pinto JE (2014b) Shading levels and net type on vegatative growth and essential oil production of peppermint. Hort Bras 32:194–199. https://doi.org/10.1590/S010205362014000200013
Ferraz TDO, Ferreira DQ, Mourão RHV, Formiga FR, Carvalho JCT, Fernandes CP (2020) Nano-emulsification of Aeollanthus suaveolens Mart. Ex Spreng essential oil modifies its neuroeffects? Drug Deliv Transl Res 10(6):1764–1770. https://doi.org/10.1007/s13346-020-00846-w
Ferreira DF (2011) Sisvar: a computer statistical analysis system. Cienc Agrotec 35:1039–1042. https://doi.org/10.1590/S1413-70542011000600001
Ferreira LR, Martins ACCT, Palheta IC (2017) Ethnopharmacological and phytochemicalsaspects of the Aeollanthus suaveolens Mart. Ex Spreng. In: Alfaro ATS, Trojan DG (eds) Environmental sciences and sustainable development in the amazon. Atena, Curitiba, pp 77–88
Harley RM (2012) Checklist and key of genera and species of the Lamiaceae of the Brazilian amazon. Rodriguésia 63:129–144. https://doi.org/10.1590/S2175-78602012000100010
Hassankhah A, Vahdati K, Lotfi M, Mirmasoumi M, Preece J, Assareh MH (2014) Effects of ventilation and sucrose concentrations on the growth and plantlet anatomy of micropropagated Persian walnut plants. Int J Hortic Sci Technol 1(2):111–120
Kozai T (2010) Photoautotrophic micropropagation-environmental control for promoting photosynthesis. Propag Ornam Plants 10(4):188–204
Kozai T, Xiao Y (2008) A commercialized photoautotrophic micropropagation system. In: Gupta SD, Ibaraki Y (eds) Plant tissue cult eng. Springer, Netherlands, Dordrecht, pp 355–371
Kozai T, Oki H, Fujiwara K (1990) Photosynthetic characteristics of Cymbidium plantlet in vitro. Plant Cell Tissue Organ Cult 22(3):205–211. https://doi.org/10.1007/BF00033638
Lazzarini LES, Bertolucci SKV, de Carvalho AA, Santiago AC, Pacheco FV, Yucesan B, Pinto JEBP (2019) Explant type and natural ventilation systems influence growth and content of carvacrol and thymol of Lippia gracilis Schauer. Plant Cell Tissue Organ Cult 137(1):33–43. https://doi.org/10.1007/s11240-018-01548-5
Moreira AL, Silva ABd, Santos A, Reis COd, Landgraf PRC (2013) Cattleya walkeriana growth in different micropropagation systems. Cienc Rural 43:1804–1810. https://doi.org/10.1590/S0103-84782013001000012
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio sssays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ngo-Mback MNL, Famewo EB, MubarakAli D, Eke P, Thajuddin N, Afolayan AJ, Jazet Dongmo PM, Fekam Boyom F (2019) An investigation of chemical composition and antimicrobial activity of essential oils extracted from Aeollanthus and Plectranthus species. Biocatal Agric Biotechnol 22:101412. https://doi.org/10.1016/j.bcab.2019.101412
Righetti B, Magnanini E, Infante R, Predieri S (1990) Ethylene, ethanol, acetaldehyde and carbon dioxide released by Prunus avium shoot cultures. Physiol Plant 78(4):507–510. https://doi.org/10.1111/j.1399-3054.1990.tb05234.x
Rocha TT, Araújo DX, da Silva AM, de Oliveira JPV, de Carvalho AA, Gavilanes ML, Bertolucci SKV, Alves E, Pinto JEBP (2022) Morphoanatomy and changes in antioxidant defense associated with the natural ventilation system of micropropagated Lippia dulcis plantlets. Plant Cell Tissue Organ Cult 151(3):467–481. https://doi.org/10.1007/s11240-022-02364-8
Rodrigues M, Costa THF, Festucci-Buselli RA, Silva LC, Otoni WC (2012) Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.). In vitro cell. Dev Biol Plant 48(1):67–72. https://doi.org/10.1007/s11627-011-9398-8
Saldanha CW, Otoni CG, de Azevedo JLF, Dias LLC, do Rêgo MM, Otoni WC (2012) A low-cost alternative membrane system that promotes growth in nodal cultures of Brazilian ginseng [Pfaffia glomerata (Spreng) Pedersen]. Plant Cell Tissue Organ Cult 110(3):413–422. https://doi.org/10.1007/s11240-012-0162-5
Saldanha CW, Otoni CG, Rocha DI, Cavatte PC, Detmann KDSC, Tanaka FAO, Dias LLC, DaMatta FM, Otoni WC (2014) CO2-enriched atmosphere and supporting material impact the growth, morphophysiology and ultrastructure of in vitro Brazilian-ginseng [Pfaffia glomerata (Spreng) Pedersen] plantlets. Plant Cell Tissue Organ Cult 118(1):87–99. https://doi.org/10.1007/s11240-014-0464-x
Scalon MC, Haridasan M, Franco AC (2017) Influence of long-term nutrient manipulation on specific leaf area and leaf nutrient concentrations in savanna woody species of contrasting leaf phenologies. Plant Soil 421(1):233–244. https://doi.org/10.1007/s11104-017-3437-0
Schoh PG, Lefevre B, Teisson C, Ganry J (1989) Photosynthèse et respiration de bananier in vitro. Photosynthetica 23(1):113–118
Schuelter AR, da Luz CL, Scherer AM, de Souza CS, Stefanello S (2015) Light availability, type of sealing and flask on germination and initial growth in vitro of plantlets cubiu (Solanum sessiliflorum Dunal). Sci Agrar Parana 14(3):183–190
Siahsar B, Rahimi M, Tavassoli A, Raissi A (2011) Application of biotechnology in production of medicinal plants. Am Eurasian J Agric Environ Sci 11(3):439–444
Silva ST, Bertolucci SKV, da Cunha SHB, Lazzarini LES, Tavares MC, Pinto JEBP (2017) Effect of light and natural ventilation systems on the growth parameters and carvacrol content in the in vitro cultures of Plectranthus amboinicus (Lour) Spreng. Plant Cell Tissue Organ Cult 129(3):501–510. https://doi.org/10.1007/s11240-017-1195-6
Simionatto E, Porto C, Stüker CZ, Dalcol II, Silva UFd (2007) Chemical composition and antimicrobial activity of the essential oil from Aeolanthus suaveolens Mart. ex Spreng. Quim Nova 30:1923–1925. https://doi.org/10.1590/S0100-40422007000800024
Souza GSd, Silva JdS, de Oliveira UC, dos Santos Neto RB, dos Santos AR (2014) Vegetative growth and yield of essential oil of the rosemary plants development when cultivated under colored screens. Biosci J 30(3):232–239
Vahdati K, Hassankhah A (2014) Developing a photomixotrophic system for micropropagation of Persian walnut. Acta Hortic 1050:181–187. https://doi.org/10.17660/ActaHortic.2014.1050.23
van Den Dool H, Kratz DK (1963) A generalization of the retention index system including linear temperature programmed gas—liquid partition chromatography. J Chromat A 11:463–471. https://doi.org/10.1016/S0021-9673(01)80947-X
Veloso DJ, Abrão F, Martins CHG, Bronzato JD, Gomes BPFA, Higino JS, Sampaio FC (2020) Potential antibacterial and anti-halitosis activity of medicinal plants against oral bacteria. Arch Oral Biol 110:104585. https://doi.org/10.1016/j.archoralbio.2019.104585
Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144(3):307–313. https://doi.org/10.1016/S0176-1617(11)81192-2
Xiao Y, Niu G, Kozai T (2011) Development and application of photoautotrophic micropropagation plant system. Plant Cell Tissue Organ Cult 105(2):149–158. https://doi.org/10.1007/s11240-010-9863-9
Zobayed SMA, Armstrong J, Armstrong W (2001) Leaf anatomy of in vitro tobacco and cauliflower plantlets as affected by different types of ventilation. Plant Sci 161(3):537–548. https://doi.org/10.1016/S0168-9452(01)00438-1
Acknowledgements
The authors would like to thank the National Council for Scientific and Technological Development (CNPq—Conselho Nacional de Desenvolvimento Científico e Tecnológico), the Coordination for the Improvement of Higher Education Personnel (CAPES—Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and the Minas Gerais State Research Foundation (FAPEMIG—Fundação de Pesquisa do Estado de Minas Gerais) for financial support (scholarships and research grants).
Funding
The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.
Author information
Authors and Affiliations
Contributions
All authors contributed substantially to the work reported. DXA: writing of the manuscript, investigation. TTR: investigation. AAC: data analysis, graphic design. APRM: investigation. FNSR: investigation. RMAA: investigation. SKVB: concept of the study, chemical analyses. JEBPP: concept of the study, writing of the manuscript, project administration.
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Communicated by Konstantin V. Kiselev.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Araújo, D.X., Rocha, T.T., de Carvalho, A.A. et al. Volatile organic compound (VOC) profile and plantlet growth of Aeollanthus suaveolens under conventional and alternative membrane systems. Plant Cell Tiss Organ Cult 153, 333–342 (2023). https://doi.org/10.1007/s11240-023-02469-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-023-02469-8