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Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars

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Abstract

Conservation of plant species, particularly of those important for agriculture, has now reached a very high importance in order to ensure sustainable utilization of biological resources by preventing further losses of plant diversity. The most advanced method for plant conservation is cryopreservation. Cryotherapy that relies on cryopreservation procedure, alone or in combination with other techniques, can be used for pathogen elimination from infected plants. Viral diseases pose a significant threat to the agriculture, decreasing yields and crop quality. In this work, two cryotherapy methods (D and V cryo-plate) were evaluated for plum pox virus (PPV) eradication from autochthonous plums (Prunus domestica L.) ‘Crvena Ranka’ and ‘Belošljiva’, widely present under different local names on the Balkan peninsula. Nine different cryo-plate treatments were performed per genotype. Cryopreservation was effective in PPV elimination from variety ‘Crvena Ranka’ in three cryo-treatments. PPV elimination was, however, not achieved in variety ‘Belošljiva’ by any of the two methods (D and V-cryoplate) and treatments applied.

Key message

Eradication of plum pox virus from infected plums is possible by cryotherapy methods, but the efficiency is highly dependent on the treatment applied and the given genotype.

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Funding

This research was supported by the Science Fund of the Republic of Serbia, PROMIS, #6062279, project Conservation and plum pox eradication from Serbian autochthonous plum genotypes using cryotechniques – CryoPlum and by the Ministry of Education, Science and Technological Development of the Republic of Serbia, contract 451-03-68/2022-14/200215.

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Authors and Affiliations

  1. Fruit Research Institute, Kralja Petra I 9, 32102, Čačak, Serbia

    Darko Jevremović, Bojana Vasilijević, Tatjana Anđelić & Tatjana Vujović

Authors
  1. Darko Jevremović
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  2. Bojana Vasilijević
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  3. Tatjana Anđelić
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  4. Tatjana Vujović
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Contributions

DJ—conceptualization, methodology, investigation, data curation, writing—original draft preparation, writing—original draft preparation, review and editing, supervision, project administration, and funding acquisition; BV—investigation; TA—investigation; TV—conceptualization, methodology, investigation, data curation, and writing—original draft preparation; all authors read the manuscript, edited and approved the final version.

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Correspondence to Darko Jevremović.

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Communicated by Christell van der Vyver.

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Jevremović, D., Vasilijević, B., Anđelić, T. et al. Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars. Plant Cell Tiss Organ Cult 152, 529–538 (2023). https://doi.org/10.1007/s11240-022-02425-y

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