Abstract
DNA binding with one finger (Dof) is a plant-specific transcription factor (TF) that has a key role in seed development and germination, light-mediated control, and plant responses to biotic and abiotic stressors. Herein, we aimed to evaluate the role of the soybean Dof -TFs in plant tolerance breeding. In this study, GmDof4.2 was isolated from the soybean. GmDof4.2 in soybean seedlings responded to drought, salt, heat, and cold stresses, according to the results of real-time fluorescence quantitative PCR (qPCR). GmDof4.2 protein had a 59-amino-acid Dof domain with a CX2CX21CX2C motif and four conserved cysteine (Cys) residues, according to the predicted amino acid sequence. The GmDof4.2 protein shared the most genetic similarities with the Cajanus cajan CcDof4 protein. Seven cis-elements associated with stress or hormone induction, as well as two TF binding sites, were found in the GmDof4.2 promoter region. GmDof4.2 protein bound specifically to the AAAG motif in vivo according to the results of yeast one-hybrid. Moreover, the overexpression of GmDof4.2 resulted in higher soluble carbohydrates content and proline content and lower relative electrolyte leakage and malondialdehyde content in transgenic tobacco than in wild-type (WT) tobacco under drought stress. Furthermore, the expression levels of nine stress-related genes were considerably greater in transgenic tobacco than in WT tobacco. The underlined data suggested that the GmDof4.2 improved drought tolerance in transgenic tobacco.
Key Message
A Dof transcription factor, GmDof4.2, was isolated from soybean. The expression of GmDof4.2 could be induced by abiotic stress. Overexpression of GmDof4.2 improved drought tolerance in transgenic tobacco.
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Abbreviations
- 3-AT:
-
3-Amino-1,2,4-triazole
- ABA:
-
Abscisic acid
- Cys:
-
Cysteine
- Dof:
-
DNA binding with one finger
- His:
-
Histidine
- Leu:
-
Leucine
- MeJA:
-
Methyl jasmonate
- PCR:
-
Polymerase chain reaction
- PEG:
-
Polyethylene glycol
- qPCR:
-
Quantitative real-time PCR
- TF:
-
Transcription factor
- Trp:
-
Tryptophan
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- WT:
-
Wild-type
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Acknowledgements
This work was supported by the Basic Scientific Research Funds of Heilongjiang Provincial colleges and universities (No. 145109506) and the National Natural Science Foundation of China (No. 32101694) .
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Funding was supported by Basic Scientific Research Funds of Heilongjiang Provincial colleges and universities, (Grand No. 145109506), Natural Science Foundation of China, (Grand No. 32101694).
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Conceived and designed the experiments: SL and YZ. Performed the experiments: YZ, JC, JH, JZ, WS, HY, YZ, TM, TS, and MZ. The first draft of the manuscript was written by YZ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Zhai, Y., Chen, J., He, J. et al. Isolation, characterization and functional validation of a soybean transcription factor, GmDof4.2 improves drought tolerance in transgenic tobacco. Plant Cell Tiss Organ Cult 152, 357–367 (2023). https://doi.org/10.1007/s11240-022-02412-3
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DOI: https://doi.org/10.1007/s11240-022-02412-3