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ThDof1.4 and ThZFP1 constitute a transcriptional regulatory cascade involved in salt or osmotic stress in Tamarix hispida

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Abstract

Identification of the upstream regulators of a gene is important to characterize the transcriptional pathway and the function of the gene. Previously, we found that a zinc finger protein (ThZFP1) is involved in abiotic stress tolerance of Tamarix hispida. In the present study, we further investigated the transcriptional pathway of ThZFP1. Dof motifs are abundant in the ThZFP1 promoter; therefore, we used them to screen for transcriptional regulators of ThZFP1. A Dof protein, ThDof1.4, binds to the Dof motif specifically, and was hypothesized as the upstream regulator of ThZFP1. Further study showed that overexpression of ThDof1.4 in T. hispida activated the expression of GUS controlled by the ThZFP1 promoter. In T. hispida, transient overexpression of ThDof1.4 increased the transcripts of ThZFP1; conversely, transient RNAi-silencing of ThDof1.4 reduced the expression of ThZFP1. Chromatin immunoprecipitation indicated that ThDof1.4 binds to the ThZFP1 promoter. Additionally, ThDof1.4 and ThZFP1 share similar expression patterns in response to salt or drought stress. Furthermore, like ThZFP1, ThDof1.4 could increase the proline level and enhance ROS scavenging capability to improve salt and osmotic stress tolerance. Together, these results suggested that ThDof1.4 and ThZFP1 form a transcriptional regulatory cascade involved in abiotic stress resistance in T. hispida.

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Funding

The funding was provided by the project of the culture of the young scientists in scientific innovation in Xinjiang Province (Grant No. 201371101), Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Grant No. 2013A05) and Fundamental Research Funds for the Central Universities (Grant No. 2572015AA08).

Author contributions

YW designed research and wrote the paper. DZ performed research. DZ analyzed data and wrote the paper. LW and YZ contributed to genes expression analysis. HZ cloned the gene. All the authors reviewed the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China

    Dandan Zang, Lina Wang, Yiming Zhang, Huimin Zhao & Yucheng Wang

  2. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, Xinjiang, China

    Yucheng Wang

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  1. Dandan Zang
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  2. Lina Wang
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  3. Yiming Zhang
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  4. Huimin Zhao
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  5. Yucheng Wang
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Correspondence to Yucheng Wang.

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Zang, D., Wang, L., Zhang, Y. et al. ThDof1.4 and ThZFP1 constitute a transcriptional regulatory cascade involved in salt or osmotic stress in Tamarix hispida . Plant Mol Biol 94, 495–507 (2017). https://doi.org/10.1007/s11103-017-0620-x

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  • DOI: https://doi.org/10.1007/s11103-017-0620-x

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