Abstract
Rain forest in Brazil is home of around 50% of the world’s plant biodiversity and many species have a high economical potential. Butia eriospatha is an endemic species of the Brazilian Atlantic Forest and due to the anthropic intervention, it is in a vulnerable conservation status. In this species, plants are only propagated sexually by seeds which show dormancy and have a low germination rate, combined with slow seedling emergence. To manage the limitation of the seedling establishment and conservation strategies of this species, the viability, antioxidant activity, and profiles of polyamines and amino acids of desiccated B. eriospatha embryos were assessed. Mature and desiccated embryos were germinated under in vitro conditions on Murashige and Skoog culture medium, which was supplemented with hormones and antioxidants. Embryo desiccation tolerance threshold was 0.14 g H2O g DW−1 with a germination rate of 93.3%. During embryos desiccation was observed a significant increase in putrecine (PUT). Increase in guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) activity led us to suggest that they are the main enzymes involved in cellular protection during desiccation. An increase of amino acids content, especially glutamic acid (Glu), leucine (Leu), lysine (Lys), glutamine (Gln), which are known as osmoprotectors also was observed. Embryos previously desiccated to 0.14 g H2O g DW−1 were successfully cryopreserved, and showed a post-thawing recovery rate of 85%. The physiological and biochemistry approach of this study associated to a cryopreservation protocol can be used for plant genetic resources conservation of other Arecaceae species in the world.
Key message
In vitro germination and the cryopreservation protocol of B. eriospatha embryo are directly associated with water content threshold and cell biochemistry state.
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Data availability
The data are available from the corresponding author on reasonable request.
Abbreviations
- DAH:
-
1,7-Diaminoheptane
- APX:
-
Ascorbate peroxidase
- CAT:
-
Catalase
- DW:
-
Dry weight
- EDTA:
-
Ethylenediamine tetraacetic acid
- FW:
-
Fresh weight
- GSI:
-
Germination Speed Index
- GPX:
-
Guaiacol peroxidase
- MC:
-
Moisture content
- NBT:
-
Nitroblue tetrazolium
- ROS:
-
Reactive Oxygen Species
- SOD:
-
Superoxide Dismutase
- SPD:
-
Spermidine
- SPM:
-
Spermine
- TCA:
-
Trichloroacetic acid
- TTC:
-
Tetrazolium test
- PAs:
-
Polyamines
- PEG 6000:
-
Polyethylene glycol 6000
- PVP:
-
Polyvinylpyrrolidone
- PUT:
-
Putrescine
- WC:
-
Water content
- Ψw :
-
Water potential
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Acknowledgements
The authors acknowledge the staff of the Laboratory of Morphogenesis and Plant Biochemistry of the Federal University of Santa Catarina, Brazil. The authors also acknowledge Maycon Moraes da Silva for assisting in the collection of fruits. This work was supported by the National Council for Scientific and Technological Development (CNPq, Brazil) Proc. Nº (311156/2017-7 457940/2014-0) and the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil)—Finance Code 001.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior,001,Neusa Steiner,001,Daniela Goeten,001,Rosa Angélica Elias
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DG, NS and CW: contributed to planning the experiments, analyzing the data, as well as writing and editing the manuscript. NS: coordinated the study. DG collected the mature seeds. DG, RAE and LGP: performed the experiments. NS, MPG and DG: contributed to the writing of the manuscript.
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Goeten, D., Elias, R.A., Polesi, L.G. et al. Effect of water content and biochemical cell state on the germination rate of cryopreserved Butia eriospatha embryos (Arecaceae) . Plant Cell Tiss Organ Cult 152, 339–356 (2023). https://doi.org/10.1007/s11240-022-02411-4
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DOI: https://doi.org/10.1007/s11240-022-02411-4