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Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures

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Abstract

The aim of the present work was to determine the effects of exogenous polyamines (PAs) supplemented into a BM medium free of plant growth regulators (PGR), on the endogenous PAs, IAA and ABA contents and growth of A. angustifolia embryogenic cultures. The addition of PAs to the PGR-free BM medium, enhanced the growth of embryogenic cultures. PAs endogenous metabolism was significantly affected by exogenous PAs. PAs supplemented to a PGR-free BM medium, mainly Put and Spm, increased the endogenous IAA and ABA levels, showing a direct relationship -between PAs levels and ABA accumulation. These results direct new strategies to further improve A. angustifolia somatic embryogenesis, especially during the maturation phase, by culture medium manipulation using exogenous PAs.

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Abbreviations

BM:

Culture medium of Gupta and Pullman (1991)

FM:

Fresh mass

PAs:

Polyamines

PGR:

Plant growth regulators

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

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Acknowledgements

This research was carried out with financial support from the National Council for Scientific and Technological Development (CNPq) and the State of São Paulo Research Foundation (FAPESP).

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Authors and Affiliations

  1. Plant Developmental Physiology and Genetic Laboratory, Department of Plant Science, Federal University of Santa Catarina, CP 476, Florianopolis, Santa Catarina, 88034-001, Brazil

    Neusa Steiner & Miguel P. Guerra

  2. Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, Sao Paulo, 05422-970, Brazil

    Claudete Santa-Catarina, Vanildo Silveira & Eny I. S. Floh

Authors
  1. Neusa Steiner
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  2. Claudete Santa-Catarina
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  3. Vanildo Silveira
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  4. Eny I. S. Floh
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  5. Miguel P. Guerra
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Correspondence to Miguel P. Guerra.

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Steiner, N., Santa-Catarina, C., Silveira, V. et al. Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures. Plant Cell Tiss Organ Cult 89, 55–62 (2007). https://doi.org/10.1007/s11240-007-9216-5

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  • DOI: https://doi.org/10.1007/s11240-007-9216-5

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