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Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation

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Abstract

In the current study, a simple cryopreservation method (desiccation) was applied to Corylus avellana L. callus. Accordingly, the effects of abscisic acid (ABA) concentration, pretreatment duration on MS medium containing ABA + 10% sucrose, and storage length in liquid nitrogen (LN) on the callus survival rate were investigated. Calli’s survival was assessed 8 weeks after exiting from LN. Callus survival after cryopreservation was significantly affected by the concentration and duration of the treatment with ABA-supplemented media. However, storage duration in LN did not have an impact on the callus survival rate. In the present study, the highest survival rate (45.79%) was obtained in both treatments of 20 days preculture on medium containing 2 mg l− 1 ABA following 2 h desiccation-one day storage in LN and 2.5 h desiccation-thirty days storage in LN.

Key message

The desiccation method with the help of ABA and sucrose was an effective method in the successful cryopreservation of Corylus avellana L. callus.

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Acknowledgements

The authors gratefully acknowledge the Research Deputy of Tarbiat Modares University, Tehran, for the financial support of this research project.

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Authors and Affiliations

  1. Department of Plant Genetics and Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Shiva Mojarrad Nanas & Ahmad Moieni

  2. Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Ali Mokhtassi-Bidgoli

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  1. Shiva Mojarrad Nanas
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  2. Ahmad Moieni
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Contributions

SMN carried out all experiments interpreted the results and wrote the manuscript. AM supervised the whole project by supplying the materials and equipments, designing the experiments, and editing the manuscript. AMB performed data analysis. All authors read and approved the final manuscript.

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Correspondence to Ahmad Moieni.

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Communicated by Qiao-Chun Wang.

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Mojarrad Nanas, S., Moieni, A. & Mokhtassi-Bidgoli, A. Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation. Plant Cell Tiss Organ Cult 152, 267–273 (2023). https://doi.org/10.1007/s11240-022-02401-6

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