Abstract
The present study describes a simple, reproducible, and cost-effective regeneration system using seaweed liquid extract (SLE) of Sargassum ilicifolium supplemented in Murashige and Skoog (MS) medium for in vitro seed germination, multiple shoot and root induction, and plumbagin accumulation in P. zeylanica. High rate of seed germination and seedling length was recorded on ½ − MS + SLE (2%) which was 1.11- and 1.28-fold higher as compared to GA3 (2.89 µM). The best response in terms of explants producing maximum number of shoots was recorded on MS medium supplemented with 4% SLE that was 2.26- and 1.37-fold higher than BAP (3.33 µM) alone and in combination with IAA (4.28 µM), respectively. High frequency root induction and plumbagin accumulation was recorded on ½ − MS + 2.5% SLE which showed 1.34-, 1.49-, and 1.44-fold increase for the roots per shoots, average root length, and plumbagin, respectively when compared with IBA (6.15 µM). Besides being an alternative to commercially available PGRs, this is the first report demonstrating the potential of SLE for enhanced growth and plumbagin accumulation in P. zeylanica.
Key message
Efficient in vitro mass propagation protocol of P. zeylanica is developed using S. ilicifolium SLEs. SLEs with MS media were a substitute to the commercially available PGRs for micro-propagation and plumbagin production.
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Abbreviations
- ANOVA:
-
Analysis of variance
- BAP:
-
6-Benzylaminopurine
- DDW:
-
Double distilled water
- DMRT:
-
Duncan’s multiple range test
- GA3:
-
Gibberellic acid
- HPTLC:
-
High performance thin layer chromatography
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- Kin:
-
Kinetin
- MS:
-
Murashige and Skoog
- NAA:
-
1-Naphthaleneacetic acid
- PGRs:
-
Plant growth regulators
- SLEs:
-
Seaweed liquid extracts
- Zea:
-
Zeatin
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Acknowledgements
The author P. S. Kakade sincerely thanks the Department of Science and Technology, Ministry of Science and Technology, Government of India for the research grant under Women Scientist Scheme (WOS–A) (SR/WOS-A/LS-367/2012). P. S. Kakade also thanks Dr. Virdhaval M. Nalavade, Assistant Professor, Department of Biotechnology, Yashwantrao Chavan Institute of Science, Satara, Maharashtra for providing the S. ilicifolium extracts.
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Department of Science and Technology, Ministry of Science and Technology, Government of India under Women Scientist Scheme (WOS–A) (SR/WOS-A/LS-367/2012).
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Study conception and design was done by PSK and NPM. PSK and SBZ performed the experiments. Analysis and interpretation of data was done by PSK. Drafting of manuscript was done by PSK and SBZ. Critical revision was done by NPM. All authors read and approved the final manuscript.
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Kakade, P.S., Zimare, S.B. & Malpathak, N.P. Effects of Sargassum ilicifolium seaweed extract on enhanced in vitro seed germination, mass propagation, and accumulation of plumbagin in Plumbago zeylanica L.. Plant Cell Tiss Organ Cult 149, 399–410 (2022). https://doi.org/10.1007/s11240-022-02242-3
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DOI: https://doi.org/10.1007/s11240-022-02242-3