Summary
A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced on Murashige and Skoog's basal medium supplemented with 27.2 μM adenine sulfate +2.46 μM indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing 4.92 μM IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant shoot) within a period of 5 mo., eight plantlets could be raised. After a hardening period of 4 wk, there was a 90% transplantation success in the field compared to the 60–65% survival of plantlets recorded in the experiments of previous workers. The plantlets derived through in vitro propagation mimic the growth and morphological characteristics of the donor plants.
Similar content being viewed by others
References
Balakumar, T.; Selvakumar V. Certain preliminary observations of the micropropagation of the antidiabetic herb Gymnema sylvestre R.Br. In: De, A. D., ed. Recent trends in spices and medicinal plants research, New Delhi: Associated; 2000:92–95.
Balakumar, T.; Selvakumar, V.; Sriganesan, T.; Anbudurai, P. R.; Stephen, D.; James, M. R. Medicinal plants facing threat in the Azhagar Hills (Eastern Ghats) of Madurai, In: Ramamurthy, R.; Sudarsanam, G., eds. Natural medicines for human welfare, Tirupathi, India. In press.
Barve, D. M.; Mehta, A. R. Clonal propagation of mature elite trees of Commiphora wightii. Plant Cell Tiss. Organ Cult. 35:237–244; 1993.
Chopra, R. N.; Nayar, S. L.; Chopra, I. C. Glossary of Indian medicinal plants. New Delhi. NISCOM Publishers; 1996.
Chu, I. Y. E. Perspectives of micropropagation industry. In: Kurata, K.; Kozai, T., eds Transplant production systems. Amsterdam: Kluwer Academic; 1992;137–150.
Duncan, D. B. Multiple range and multiple F test. Biometrics 11:1–42; 1955.
Gradner, U. Phimbago indica, choice of grwoth media, fertilization and in vitro propagation. Deutscher-Gartenbau 45:358–359; 1991.
Hu, C. Y.; Wang, P. J. Meristem, shoot tip and bud culture. In: Evans, D. A.; Sharp, W. R.; Ammirato, P. V.; Yamada, Y., eds. Handbook of plant cell culture, vol. 1 New York: Macmillan; 1983:177–227.
Kumar, K. S.; Bhavanandan, K. V. Micropropagation of Plumbago rosea Linn. Plant Cell Tiss. Organ Cult. 15:275–278; 1988.
Lin, C. S.; Chang, W. C. Micropropagation of Bambusa edulis through nodal explants of field-grown culms and flowering of regenerated plantlets. Plant Cell Rep. 17:617–620; 1998.
Malathy, S.; Pai, J. S. Micropropagation of Ixora singaporensis (Linn.)—an ornamental shrub. Curr. Sci. 75:545–547; 1998.
Mantell, S. H.; Matthews, J. A.; McKee, R. A. Principles of plant biotechnology. Boston, Blackwell Scientific; 1985:130–157.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–479; 1962.
Patnaik, K.; Debata, B. K. Micropropagation of Hemidesmus indicus (L.) R.BR. through axillary bud culture. Plant Cell Rep. 15:427–430; 1996.
Pattnaik S. K.; Sahoo, Y.; Chand, P. K. Micropropagation of a fruit tree Morus australis Poir. Syn. M., acidosa Griff. Plant Cell Rep. 15:841–845; 1996.
Pierik, R. L. M. In vitro culture of higher plants. Dordrech. Martinus Nijhoff; 1978:183–230.
Quraishi, A.; Koche, V.; Mishra, S. K. In vitro micropropagation from nodal segments of Cleistanthus collinus. Plant Cell Tiss. Organ Cult. 45:87–91; 1996.
Sriganesan, T. Flora of Azhagar Hills. Ph.D. thesis, Madurai Kamaraj University, Madurai India: vols. I and II; 1984.
Sudha, C. G.; Seeni, S. In vitro propagation of Rauwolfia micrantha, a rare medicinal plant. Plant Cell Tiss. Organ Cult. 44:243–248; 1996.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Selvakumar, V., Anbudurai, P.R. & Balakumar, T. In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants. In Vitro Cell.Dev.Biol.-Plant 37, 280–284 (2001). https://doi.org/10.1007/s11627-001-0050-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11627-001-0050-x