Abstract
Taxifolin in larch is an important natural active ingredient, which prevents and treats liver diseases and cardiovascular diseases, and has many health benefits and application values such as anti-tumor, anti-virus, and anti-aging. However, the traditional extraction method is not conducive to the protection of the ecological environment, and also limits the industrial production of taxifolin from larch. Therefore, the purpose of this research is to establish a larch callus culture system and obtain taxifolin from callus. The shoots of the stem-tips of Larix olgensis were used as explants to be cultured. The light conditions, optimal medium and hormone ratio for callus induction were screened to evaluate the callus induction characteristics of larch and its influence on the accumulation of taxifolin. The results showed that: callus formation started on 28 ~ 51 days, and the best conditions for inducing callus were explored: 12 h/d light culture MS + 2,4-D 2 mg/L + 6-BA 1 mg/L + KT 0.1 mg/L; The callus induction rate was 50-80 %; The hormone combination with the highest accumulation of taxifolin was 6-BA 1 mg/L + NAA 0.1 mg/L, reaching 0.477 . In conclusion, this study revealed a suitable induction condition for larch callus culture and taxifolin accumulation.
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Acknowledgements
The authors are thankful to professor Liu Wencong, College of traditional Chinese medicine, Jilin Agricultural University, and the straw biological utilization platform of the Ministry of education for their technical support.
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This work were supported by the Changchun Science and Technology Bureau of Jilin Province (NO. 17YJ013).
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WL perceived and planned the experiment; XL, YZ and YZ transcribed the chief document text and investigated figures; CX and CD mainly performed statistics and figures drawing; WM, LD, QD and SX checked and modified the manuscript. All authors review and legalize the content of the manuscript.
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Xinglong Liu and Yingchun Zhao have contributed equally to this work.
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Liu, X., Zhao, Y., Chen, X. et al. Establishment of callus induction system, histological evaluation and taxifolin production of Larch. Plant Cell Tiss Organ Cult 147, 467–475 (2021). https://doi.org/10.1007/s11240-021-02139-7
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DOI: https://doi.org/10.1007/s11240-021-02139-7