Abstract
Primordial shoot explants excised from buds of one Larix decidua tree, about 30 years old, produced more adventitious buds, elongating into shoots, when grown on half strength Litvay medium than when grown on other basal media. Thidiazuron and N6-benzyladenine (BA) were equally effective in adventitious bud induction. In a comparative study of 30-year-old L. decidua, L. leptolepis, L. eurolepis, and L. laricina trees, explants from L. eurolepis and L. decidua produced a high number of cultures with adventitious buds that elongated into shoots; those from L. leptolepis were less productive, and those from L. laricina failed to form adventitious buds. The highest response was obtained with material collected in August and September, and in March and April; the lowest response occurred in explants from the October collection.
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Bonga, J.M., Pond, S.E. Adventitious shoot formation in cultures of 30-year-old Larix decidua, L. leptolepis, L. eurolepis, and L. laricina trees. Plant Cell Tiss Organ Cult 26, 45–51 (1991). https://doi.org/10.1007/BF00116609
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DOI: https://doi.org/10.1007/BF00116609