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Silver nanoparticles as an effective stimulant in micropropagation of Panax vietnamensis—a valuable medicinal plant

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Abstract

Effects of silver nanoparticles (AgNPs) on somatic embryogenesis and plantlets with rhizome of Panax vietnamensis were presented in this study. The highest number of somatic embryos (140) was obtained on MS medium containing 1 mg/L 2.4-D, 0.5 mg/L NAA, 0.2 mg/L Kin and 1.6 mg/L AgNPs. Plantlets with rhizome growth enhanced on SH medium supplemented with 1 mg/L NAA and 1.2 mg/L AgNPs and limited the ethylene gas content in the culture flasks. A low concentration of ethylene gas (0.92 ppm) stimulated plantlets with rhizome growth and development, and provided positive effect on the formation and quality of plantlets. The rhizome size of the plantlets in 1.2 mg/L AgNPs treatment was larger than those in the control without AgNPs. Plantlets acclimatized on the soilless substrate were higher survival rate (93.65%) as compared to the control (44.44%) after one year in the greenhouse. In particular, the rhizome saponin content was doubled compared with that in the planlets without AgNPs after 4-year planting in the greenhouse. These results may be scaled up for micropropagation of this important medicinal plant.

Key Message

AgNPs improved somatic embryogenesis. AgNPs enhanced plantlets with rhizome. AgNPs limited ethylene accumulation during micropropagation. AgNPs increased the survival rate of plantlets with rhizome in the greenhouse. AgNPs increased saponin content of plantlets with rhizome.

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Abbreviations

MS:

Murashige and Skoog (1962)

SH:

Schenk and Hildebrandt (1972)

Kin:

Kinetin

NAA:

1-Naphthaleneacetic acid

AgNPs:

Silver nanoparticles

2.4-D:

2,4 Dichlorophenoxyacetic acid

TDZ:

Thidiazuron

G-Rg1:

Gingsenosid Rg1

M-R2:

Majonoside-R2

G-Rb1:

Gingsenosid Rb1

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Acknowledgements

The authors would like to thank Professor C.C. Chinnappa (University of Calgary, Canada) for critical reading of the manuscript. This research is funded by Vietnam Academy of Science and Technology under grant number NVCC22.01/21-21 (No. 2510/QĐ-VHL) and Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106.01-2019.301.

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Authors and Affiliations

  1. Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

    Do Manh Cuong, Hoang Thanh Tung, Ha Thi My Ngan, Vu Quoc Luan, Truong Hoai Phong, Hoang Dac Khai & Duong Tan Nhut

  2. University of Sciences, Hue University, Hue, Vietnam

    Do Manh Cuong & Truong Thi Bich Phuong

  3. Lam Dong Department of Science and Technology, Lam Dong, Vietnam

    Phan Cong Du

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  1. Do Manh Cuong
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Contributions

Do Manh Cuong acquired data wrote the manuscript. Phan Cong Du, Hoang Thanh Tung, Ha Thi My Ngan, Vu Quoc Luan, Truong Hoai Phong, Hoang Dac Khai, Truong Thi Bich Phuong participated in interpretation of data and revision for intellectual content. Duong Tan Nhut conceptualized and designed the study. All authors discussed the results and contributed to the final manuscript.

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Correspondence to Duong Tan Nhut.

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Manh Cuong, D., Cong Du, P., Tung, H.T. et al. Silver nanoparticles as an effective stimulant in micropropagation of Panax vietnamensis—a valuable medicinal plant. Plant Cell Tiss Organ Cult 146, 577–588 (2021). https://doi.org/10.1007/s11240-021-02095-2

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