Abstract
Polycyclic aromatic hydrocarbons (PAHs) present a significant threat to human health, in which 3,4-Benzopyrene as one of the three major carcinogens has been widely studied, while there are few researches of roots differentiation under 3,4-Benzopyrene exposure for understanding the mechanism of its phytoremediation. In this paper, the adventitious root differentiation of Triarrherca sacchariflora in vitro culture seedlings was observed under 3,4-Benzopyrene exposure. The results showed that 2.0 mg/L 3,4-Benzopyrene had significant positive effects on the adventitious root differentiation, while the higher dosage of 20.0 mg/L showed inhibition effects. 3,4-Benzopyrene could cause the remarkable reduction of the endogenous IAA and the ratios of IAA/ZR, IAA/DHZR, IAA/iPA and IAA/(ZR+DHZR+iPA) in stem explants of in vitro cultural seedlings of T. sacchariflora. The responses of coleoptile elongation of Zea mays and the DR5::GUS reporter gene of Arabidopsis thaliana to 3,4-Benzopyrene exposure were all positive comparing with the controls. It could be inferred from the results that the 3,4-Benzopyrene may be an auxin-like substance. In our knowledge, it was the first report concerning the 3,4-Benzopyrene with respect to the adventitious root differentiation of T. sacchariflora in vitro culture seedlings and its auxin-like biological effect analysis. It is the meaningful step to further understanding the 3,4-Benzopyrene effects on the plant endogenous signal substances that regulate the plant growth and development which closely related to the phytoremediation of PAHs.
Key message
3,4-Benzopyrene might be an auxin-like substance with the positive biological effect on the adventitious root differentiation via regulating some endogenous hormones of in vitro cultural seedlings of T. sacchariflora.
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The datasets generated during and/or analysed during the current study are not publicly available due [REASON WHY DATA ARE NOT PUBLIC] but are available from the corresponding author on reasonable request.
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This work was supported by Natural Science Foundation of Hebei Province (Grant Number C2018204134).
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This study was funded by Natural Science Foundation of Hebei Province (grant number C2018204134).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Ran Sun and Kejiu Du. The first draft of the manuscript was written by Ran Sun and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. Ran Sun and Shuang Zhang contributed equally to the work.
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Sun, R., Zhang, S. & Du, K. Effects of 3,4-Benzopyrene on the adventitious root differentiation of in vitro culture seedlings of Triarrherca sacchariflora. Plant Cell Tiss Organ Cult 144, 641–647 (2021). https://doi.org/10.1007/s11240-020-01984-2
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DOI: https://doi.org/10.1007/s11240-020-01984-2