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GmANN, a glutathione S-transferase-interacting annexin, is involved in high temperature and humidity tolerance and seed vigor formation in transgenic Arabidopsis

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Abstract

Plant annexins are proteins with various functions, such as binding calcium ions and negatively charging lipids. Although their importance in plant stress responses has been widely reported, their function in seeds is rarely studied. A putative annexin was found in developing seed of soybean [Glycine max (L.) Merr] in our previous comparative proteomics study. In this study, it was identified as a soybean annexin, GmANN, through sequence analysis. Quantitative real time PCR (qRT-PCR) analysis indicated that the transcription levels of GmANN were increased significantly under high temperature and humidity (HTH) stress. Moreover, GmANN-transgenic Arabidopsis seeds showed greater resistance and higher seed vitality under HTH stress in contrast to the wild type (WT) seeds. GmANN overexpression plants showed increased peroxidase activities, decreased lipid peroxidation levels and decreased reactive oxygen species release levels compared to the WT plants. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that GmANN interacted with soybean glutathione S-transferase (GmGST). Taken together, our results implied that GmANN might take part in response to HTH stress and be involved in seed vigor formation in plant.

Key message

GmANN, a soybean annexin, plays an important role in response to plant HTH tolerance and in seed vigor.

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Abbreviations

ANN:

Annexin

BF:

Bright field

BiFC:

Bimolecular fluorescence complementation

CDT:

Controlled deterioration treatment

GFP:

Green fluorescent protein

GST:

Glutathione S-transferase

H2O2 :

Hydrogen peroxide

HTH:

High temperature and humidity

MDA:

Malondialdehyde

POD:

Peroxidase

PM:

Plasma membrane

qRT-PCR:

Quantitative real time PCR

TTC:

2,3,5-Triphenyltetrazolium chloride

ROS:

Reactive oxygen species

WT:

Wild type

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Acknowledgements

The authors gratefully acknowledge the partial financial support from the project supported by the Ministry of Science and Technology of China (2018YFD0100905) and the projects supported by the National Natural Science Foundation of China (31171572, 31371711, 31671772) for this research.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Jiaping Wei, Yingzi Shen, Haihong Zhao, Xiaolin Liu, Yanfeng Jia & Hao Ma

  2. Crop and Soil Sciences Department, North Carolina State University, Raleigh, NC, 27695, USA

    Xingwang Yu

Authors
  1. Jiaping Wei
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  2. Yingzi Shen
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  3. Haihong Zhao
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  4. Xiaolin Liu
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  5. Yanfeng Jia
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  6. Xingwang Yu
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  7. Hao Ma
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Contributions

The study presented here was executed in collaboration by all authors. JPW designed the experiments and performed most of the laboratory experiments; YZS and HHZ partook in gene isolation and generation of transgenic Arabidopsis; XLL took part in the yeast two-hybrid screen test; YFJ participated in vector construction; HM and XWY polished and revised this paper. All authors have approved the ultimate paper.

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Correspondence to Hao Ma.

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Communicated by Manoj Prasad.

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Wei, J., Shen, Y., Zhao, H. et al. GmANN, a glutathione S-transferase-interacting annexin, is involved in high temperature and humidity tolerance and seed vigor formation in transgenic Arabidopsis. Plant Cell Tiss Organ Cult 138, 583–595 (2019). https://doi.org/10.1007/s11240-019-01655-x

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  • DOI: https://doi.org/10.1007/s11240-019-01655-x

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