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The effects of methyl jasmonate and salicylic acid on the production of secondary metabolites in organ culture of Ziziphora persica

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Abstract

Ziziphora persica (Lamiaceae) has been used as infusions and decoctions in traditional medicine for various purposes such as sedative, carminative, and stomach pain. In the proliferation stage of shoot culture, MS medium was treated with different concentrations of methyl jasmonate (MJ) and salicylic acid (SA) (50, 100 and 150 µM) either individually or in combination with various concentrations (25 µM SA + 25 µM MJ, 25 µM SA + 50 µM MJ, 50 µM SA + 25 µM MJ, 50 µM SA + 50 µM MJ). In the present study, we investigated the quantitative changes in pigments, growth parameters, and volatile compounds in the shoot culture of nodal segments. Volatile compounds were extracted and subsequently analyzed using GC-mass spectrometry. The highest amounts of chlorophyll a and carotenoids were detected in SA25 + MJ25 treatment, whereas chlorophyll b was highly found in the control treatment. Besides, significant amounts of detected volatile compounds belonged to alkane hydrocarbons, whereas mono and sesquiterpenes were found in low amounts.

Key message

SA show higher toxic effect than MJ and affective than synergic use of elicitors on the organ culture of Z. persica. Elicitors can increase volatile compounds in different pattern compared to the previously in vivo production.

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Abbreviations

SA:

Salicylic acid

MJ:

Methyl jasmonate

References

  • Adams RP (1995) Identification of essential oils components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Illinois, pp 1–69

    Google Scholar 

  • Baque MA, Moh SH, Lee EJ, Zhong JJ, Paek KY (2012) Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol Adv 30(6):1255–1267

    Article  CAS  PubMed  Google Scholar 

  • Baytop T. (1984) Türkiye’de Bitkiler ile Tedavi. IU Yayinlari No. 3255, Eczacilik Fak. No. 40, 444 s. CSA b2

  • Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tissue Organ Cult (PCTOC) 106:279–288

    Article  CAS  Google Scholar 

  • Cui L, Wang ZY, Zhou XH (2012) Optimization of elicitors and precursors to enhance valtrate production in adventitious roots of Valeriana amurensis. ex Kom. Plant Cell Tissue Organ Cult 108:411

    Article  CAS  Google Scholar 

  • Ghahreman A (ed) (1995) Flora of Iran. Research institute of forest and rangelands. Tehran University Press, Tehran

    Google Scholar 

  • Grzegorczyk-Karolak I, Wysokińska H (2009) The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L. Herba Pol 55(3):238–243

    Google Scholar 

  • Jung S (2004) Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Plant Physiol Biochem 42:225–231

    Article  CAS  PubMed  Google Scholar 

  • Khalili M, Hasanloo T, Tabar SKK, Rahnama H (2009) Influence of exogenous salicylic acid on flavonolignans and lipoxygenase activity in the hairy root cultures of Silybum marianum. Cell Biol Int 33:988–994

    Article  CAS  PubMed  Google Scholar 

  • Lee EJ, Park SY, Paek KY (2015) Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Plant Cell Tissue Organ Cult (PCTOC) 120:1–10

    Article  CAS  Google Scholar 

  • Li X, Guo H, Qi Y, Liu H, Zhang X, Ma P, Liang Z, Liang Z, Dong J (2016) Salicylic acid-induced cytosolic acidification increases the accumulation of phenolic acids in Salvia miltiorrhiza cells. Plant Cell Tissue Organ Cult 126:333–341

    Article  CAS  Google Scholar 

  • Liu ZB, Chen J-G, Yin Z-P, Shangguan X-C, Peng D, Lu T, Lin P (2018) Methyl jasmonate and salicylic acid elicitation increase content and yield of chlorogenic acid and its derivatives in Gardenia jasminoides cell suspension cultures. Plant Cell Tissue Organ Cult 134:79–93

    Article  CAS  Google Scholar 

  • Lu H (2009) Dissection of salicylic acid-mediated defense signaling networks. Plant Signal Behav 4(8):713–717

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Manthe B, Schulz M, Schnabl H (1992) Effects of salicylic acid on growth and stomatal movements of Vicia faba L.: evidence for salicylic acid metabolization. J Chem Ecol 18:1525–1539

    Article  CAS  PubMed  Google Scholar 

  • Meral GE, Konyalioglu S, Ozturk B (2002) Essential oil composition and antioxidant activity of endemic Ziziphora taurica subsp. Cleonioides. Int J Environ Sci Dev 73:716–718

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nadaf M, Nasrabadi M, Halimi M (2012) GC-MS analysis of n-hexane extract from aerial parts of Salvia nemorosa. Middle-East J Sci Res 11:1127–1130

    Google Scholar 

  • Nadaf M, Halimi M, Nasrabadi M (2013) Identification of compounds nonpolar extract Ziziphora persica growing in Iran by GC-MS. Middle-East J Sci Res 13:187–190

    Google Scholar 

  • Nezhadali A, Shirvan BZ (2010) Separation, identification and determination of volatile compounds of Ziziphora persica bunge using HS-SPME/GC-MS. Int J Environ Sci Dev 1:115

    Article  Google Scholar 

  • Ozturk S, Ercisli S (2006) The chemical composition of essential oil and in vitro antibacterial activities of essential oil and methanol extract of Ziziphora persica Bunge. J Ethnopharmacol 106(3):372–376

    Article  CAS  PubMed  Google Scholar 

  • Popova L, Ananieva E, Hristova V, Christov K, Georgieva K, Alexieva V, Stoinova Z (2003) Salicylic acid-and methyl jasmonate-induced protection on photosynthesis to paraquat oxidative stress. Bulg J Plant Physiol 133:152

    Google Scholar 

  • Pourebad N, Motafakkerazad R, Kosari-Nasab M, Farsad Akhtar N, Movafeghi A (2015) The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica. Plant Cell Tissue Organ Cult (PCTOC) 122(2):331–339

    Article  CAS  Google Scholar 

  • Rao MV, Lee HI, Creelman RA, Mullet JE, Davis KR (2000) Jasmonic acid signaling modulates ozone-induced hypersensitive cell death. Plant Cell 12(9):1633–1646

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Roustan JP, Lotche A, Fallot J (1989) Stimulation of Daucus carota somatic embryogenesis by inhibitors of ethylene synthesis cobalt and nickel. Plant Cell Rep 8:182–185

    Article  CAS  PubMed  Google Scholar 

  • Rowshan V, Khoi MK, Javidnia K (2010) Effects of salicylic acid on quality and quantity of essential oil components in Salvia macrosiphon. J Biol Environ Sci 11:77–82

    Google Scholar 

  • Rudell DR, Mattheis JP (2002) Methyl jasmonate enhances anthocyanin accumulation and modifies production of phenolics and pigments in Fuji Apples. J Am Soc Hortic Sci 127(3):435–441

    Article  CAS  Google Scholar 

  • Sahu R, Gangopadhyay M, Dewanjee S (2013) Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides. Acta Physiol Plant 35:1473–1481

    Article  CAS  Google Scholar 

  • Sharma P, Yadav S, Srivastava A, Shrivastava N (2013) Methyl jasmonate mediates upregulation of bacoside A production in shoot cultures of Bacopa monnieri. Biotechnol Lett 35(7):1121–1125

    Article  CAS  PubMed  Google Scholar 

  • Singh PK, Chaturvedi VK, Bose B (2010) Effect of salicylic acid on seeding growth and nitrogen metabolism in cucumber (Cucumber Sativus L.). J Stress Physiol Biochem 6(3):102–113

    Google Scholar 

  • Subroto MA, Kwok KH, Hamill JD, Doran PM (1996) Coculture of genetically transformed roots and shoots for synthesis, translocation, and biotransformation of secondary metabolites. Biotechnol Bioeng 49(5):481–494

    Article  CAS  PubMed  Google Scholar 

  • Sudha G, Ravishankar G (2003) Elicitation of anthocyanin production in callus cultures of Daucus carota and involvement of calcium channel modulators. Curr Sci 84(6):775–779

    CAS  Google Scholar 

  • Szabo E, Thelen A, Peterson M (1999) Fungal elicitor preparations and methyl jasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei. Plant Cell Rep 1:485–489

    Article  Google Scholar 

  • Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci 172:861–875

    Article  CAS  Google Scholar 

  • Walker TS, Pal Bais H, Vivanco JM (2002) Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. John’s wort). Phytochemistry 60(3):289–293

    Article  CAS  PubMed  Google Scholar 

  • Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 30:313–317

    Google Scholar 

  • Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng CA Meyer. Biochem Eng J 11(2):211–215

    Article  CAS  Google Scholar 

  • Yu Z-Z, Fu C-X, Han Y-S, Li Y-X, Zhao D-X (2006) Salicylic acid enhances jaceosidin and syringin production in cell cultures of Saussurea medusa. Biotechnol Lett 28:1027–1031

    Article  CAS  PubMed  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  CAS  Google Scholar 

  • Zhao JL, Zhou LG, Wu JY (2010) Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. Appl Microbiol Biotechnol 87:137–144

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by University of Tabriz, Iran.

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Elham Zare-Hassani, Rouhollah Motafakkerazad, Jafar Razeghi & Morteza Kosari-Nasab

  2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Morteza Kosari-Nasab

Authors
  1. Elham Zare-Hassani
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  2. Rouhollah Motafakkerazad
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  3. Jafar Razeghi
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  4. Morteza Kosari-Nasab
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Contributions

R. M. and  M. K. N. conceived and designed the study; E. Z. H., R. M., J. R. and M. K. N. performed research. All authors contributed to data analysis and discussion and have read and approved the final version of the manuscript.

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Correspondence to Rouhollah Motafakkerazad.

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Communicated by Silvia Moreno.

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Zare-Hassani, E., Motafakkerazad, R., Razeghi, J. et al. The effects of methyl jasmonate and salicylic acid on the production of secondary metabolites in organ culture of Ziziphora persica. Plant Cell Tiss Organ Cult 138, 437–444 (2019). https://doi.org/10.1007/s11240-019-01639-x

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  • DOI: https://doi.org/10.1007/s11240-019-01639-x

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