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Effect of thermal shock in the development of androgenic plants of Anemone coronaria L.: influence of genotype and flower parameters

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Abstract

Anemone is an allogamous species marketed as a cut flower. Pure lines formation in this species is expensive and inefficient because progeny suffers from inbreeding depression. Pure lines obtained from plant anthers are a widely used material to produce new hybrids in short time. The aim of this study is the optimization of production of A. coronaria androgenic plants. Flower buds of eight cultivars were measured, disinfected for 20′ in 1% sodium hypochlorite and rinsed with distilled sterile water. Development stage of microspores and their size were evaluated. Anthers were distributed in Petri plates containing a double layer constituted of Nitsch and Nitsch agarized substrate enriched with activated charcoal (1%) and with or without sucrose (3%) and the same substrate in liquid phase without activated charcoal. Anthers were shocked with a preculture at 5 or 33 °C for 5 days and then incubated in the dark at 23 °C. After 21 weeks, the number of regenerants was counted. Plants were transferred into the flask with modified MS substrate and placed at 18 °C. After 3 months of in vitro culture, the survival rate of plants was evaluated. Among the varieties, significant differences in anther number, microspore size, and presence of abnormal microspores were observed. Heat shock pretreatment at 33 °C increases the number and survival of embryos and plants, but the varieties respond to the thermal shock in different ways. The influence of genotype and the maturation phase of flower buds, anthers and microspores are discussed related to the androgenic plant development.

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Acknowledgements

The authors wish to thank, Dr. Carlo Mascarello and Manuela Pamato for their precious help throughout the experiments, and Dr. Jacopo Calevo for critical reading of the manuscript. The authors also thank Biancheri Creazioni s.r.l. for partially funding the research.

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Authors and Affiliations

  1. CREA – Research Centre for Vegetable and Ornamental Crops, Corso Inglesi 508, 18038, Sanremo, IM, Italy

    A. Copetta, F. Dei, I. Marchioni, A. Cassetti & B. Ruffoni

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  1. A. Copetta
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  2. F. Dei
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  3. I. Marchioni
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  4. A. Cassetti
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  5. B. Ruffoni
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Contributions

AnC organized and performed experiment, microscopic analysis, statistical data analysis and he wrote the paper; FD cooperated in in vitro experiment; IM cooperated in data recording and manuscript revision; ArC cooperated to microscopic analysis; BR coordinated biological experiment and cooperated in paper writing.

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Correspondence to A. Copetta.

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The authors declare that they have no conflict of interest.

Additional information

Communicated by Sergio J. Ochatt.

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Copetta, A., Dei, F., Marchioni, I. et al. Effect of thermal shock in the development of androgenic plants of Anemone coronaria L.: influence of genotype and flower parameters. Plant Cell Tiss Organ Cult 134, 55–64 (2018). https://doi.org/10.1007/s11240-018-1399-4

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  • DOI: https://doi.org/10.1007/s11240-018-1399-4

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