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The effect of starch and ineubation temperature in anther culture of potato

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Abstract

Three Andean tetraploid potato genotypes (2n=48) and 7 anther-derived dihaploids (2n=24) originating from two of the tetraploids were used in anther culture. Relative number of embryos/vial was significantly higher when the anther culture media was gelatinized with 3% potato starch than when Gelrite or wheat starch (3%) were used as gelatinizing agents. The degree of anther culture response varied between tetraploids but also within a group of related dihaploids. Additionally, the embryo production of individual genotypes, tetraploids as well as dihaploids, was dependent on the incubation temperature (10, 15, 20, 25, 30°C) of the anther culture. The incubation temperature of the anther culture was also important for the regeneration rate. Direct regeneration was mostly stimulated when the anther culture was incubated at 20°C.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

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Authors and Affiliations

  1. Department of Plant Breeding, Swedish University of Agriculture Sciences, Box 7003, S-75007, Uppsala, Sweden

    Eva K. Calleberg & Lars B. Johansson

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  1. Eva K. Calleberg
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  2. Lars B. Johansson
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Calleberg, E.K., Johansson, L.B. The effect of starch and ineubation temperature in anther culture of potato. Plant Cell Tiss Organ Cult 32, 27–34 (1993). https://doi.org/10.1007/BF00040112

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  • DOI: https://doi.org/10.1007/BF00040112

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