Abstract
Beautiful vavilovia, the closest species to the common now extinct ancestor of the whole tribe Fabeae holds significant taxonomical interest and also for breeding within this group of species, which includes the most cultivated leguminous pulses in the world. In spite of this, vavilovia has attracted very scarce research to date and is in danger of complete extinction. Thus, as a part of the research carried out by an informal international group of researchers from various countries, we report here various experiments for the development and exploitation of a range of biotechnology tools for vavilovia, ranging from standard in vitro propagation, to plant regeneration from explant-derived callus, and also from protoplasts. Plants were successfully recovered following propagation from nodes, and by regeneration through organogenesis from callus derived from internodes (which provided the best responses) and leaves. Also, protoplasts were isolated from leaves and stems from in vitro shoots and from callus derived from these two explants, with the latter undergoing sustained division. Subsequently, protoplasts isolated from internode callus proliferated and also underwent organogenesis coupled with whole plant recovery at a low frequency, while protoplasts from leaf callus origin followed both organogenesis and embryogenesis simultaneously but failed to yield viable plants. Flow cytometry assessments permitted to ascertain the genetic fidelity of both propagated and regenerated plants irrespectively of the source tissue from which they were derived (i.e., either callus from explants or from protoplasts). Finally, flow cytometry also permitted us to provide the first record on the relative nuclear DNA content and genome size for Vavilovia formosa.
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Acknowledgements
SO and CC acknowledge funding from ‘Regencrop’ contract (Front de Science BAP, INRA, 2014–2018). PS acknowledges funding from Palacky University grant Agency IGA 2014_1, 2015_001, 2016_01.
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Experiments were designed and performed by SO, CC and IS. PS provided original seed material and initiated in vitro culture. All authors participated of the analyses of results and writing up of the manuscript. All co-authors agree on the contents of this manuscript and consent to submit it. The manuscript has not been submitted to any other journal for simultaneous consideration.
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The original version of this article was revised: The authors' given names and family names were presented incorrectly.
An erratum to this article is available at http://dx.doi.org/10.1007/s11240-016-1148-5.
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Ochatt, S., Conreux, C., Smýkalová, I. et al. Developing biotechnology tools for ‘beautiful’ vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential. Plant Cell Tiss Organ Cult 127, 637–648 (2016). https://doi.org/10.1007/s11240-016-1133-z
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DOI: https://doi.org/10.1007/s11240-016-1133-z