Skip to main content
SpringerLink
Log in

Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up?

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bardzik JM, HV Marsh Jr, Havis J (1971) Effects of water stress on the activities of three enzymes in maize seedlings. Plant Physiol 47(6):828–831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bouché N, Fait A, Bouchez D, Møller SG, Fromm H (2003) Mitochondrial succinic-semialdehyde dehydrogenase of the γ-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants. PNAS 100(10):6843–6848

    Article  PubMed  PubMed Central  Google Scholar 

  • Bündig C, Jozefowicz AM, Mock H-P, Winkelmann T (2016a) Proteomic analysis of two divergently responding potato genotypes (Solanum tuberosum L.) following osmotic stress treatment in vitro. J Proteom. doi:10.1016/j.jprot.2016.04.048

    Google Scholar 

  • Bündig C, Vu TH, Schum A, Meise P, Seddig S, Winkelmann T (2016b) Variability in osmotic stress tolerance of starch potato genotypes (S. tuberosum L.) as revealed by an in vitro screening: role of proline, osmotic adjustment and drought response in pot trials. J Agron Crop Sci (under revision)

  • Chaves MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought: from genes to the whole plant. Funct Plant Biol 30(3):239–264

    Article  CAS  Google Scholar 

  • Chia DW, Yoder TJ, Reiter W-D, Gibson SI (2000) Fumaric acid: an overlooked form of fixed carbon in Arabidopsis and other plant species. Planta 211:743–751

    Article  CAS  PubMed  Google Scholar 

  • Criel B, Hausman J-F, Oufir M, Swennen R, Panis B, Renaut J (2006) Proteome and sugar analysis of abiotic stress underlying cryopreservation in potato. Comm Appl Biol Sci, Ghent University 71(1):3–6

    CAS  Google Scholar 

  • Das K, Roychoudhury A (2014) Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Front Environ Sci 2:53

    Article  Google Scholar 

  • Davies WJ, Zhang J (1991) Root signal and the regulation of growth and development of plants in drying soil. Annu Rev Plant Physiol Plant Mol Biol 42:55–76

    Article  CAS  Google Scholar 

  • Delauney AJ, Verma DPS (2008) Proline biosynthesis and osmoregulation in plants. Plant J 4(2):215–223

    Article  Google Scholar 

  • El-Bashiti T, Hamamci H, Oktem HA, Yucel M (2005) Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions. Plant Sci 169:47–54

    Article  CAS  Google Scholar 

  • Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29:185–212

    Article  Google Scholar 

  • Geigenberger P, Reimholz R, Geiger M, Merlo L, Canale V, Stitt M (1997) Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit. Planta 201:502–518

    Article  CAS  Google Scholar 

  • Gibon Y, Usadel B, Blaesing OE, Kamlage B, Hoehne M, Trethewey R, Stitt M (2006) Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes. Genome Biol 7(8):Article R76

  • Gopal J, Iwama K (2007) In vitro screening of potato against water-stress mediated through sorbitol or PEG. Plant Cell Rep 26:693–700

    Article  CAS  PubMed  Google Scholar 

  • Haverkort AJ, Verhagen A (2008) Climate change and its repercussions for the potato supply chain. Potato Res 51:223–237

    Article  Google Scholar 

  • Hsiao TC, Acevedo E (1974) Plant response to water deficits, water-use efficiency, and drought resistance. Agric Meteorol 14:59–84

    Article  Google Scholar 

  • Iwama K, Yamaguchi J (2006) Abiotic stresses. In: Gopal J, Khurana SM (eds) Handbook of potato production: improvement and postharvest management. Food Product Press, New York, pp 231–278

    Google Scholar 

  • Karimi S, Yadollahi A, Arzani K (2013) Responses of almond genotypes to osmotic stress induced in vitro. J Nuts 4(4):1–7

    CAS  Google Scholar 

  • Khelil A, Menu T, Ricard B (2007) Adaptive response to salt involving carbohydrate metabolism in leaves of a salt-sensitive tomato cultivar. Plant Physiol Biochem 45:551–559

    Article  CAS  PubMed  Google Scholar 

  • Kim Y-S, Jones LS, Dong A, Kendrick BS, Chang BS, Manning MC, Randolph TW, Carpenter JF (2003) Effects of sucrose on conformational equilibria and fluctuations within the native-state ensemble of proteins. Protein Sci 12:1252–1261

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lipavska H, Vreugdenhil D (1996) Uptake of mannitol from the media by in vitro grown plants. Plant Cell Tissue Organ Cult 45:103–107

    Article  CAS  Google Scholar 

  • Lisec J, Schauer N, Kopka J, Willmitzer L, Fernie AR (2006) Gas chromatography mass spectrometry-based metabolite profiling in plants. Nat Protoc 1:387–396

    Article  CAS  PubMed  Google Scholar 

  • Luedemann A, Strassburg K, Erban A, Kopka J (2008) TagFinder for the quantitative analysis of gas chromatography and mass spectrometry (GC-MS)-based metabolite profiling experiments. Bioinformatics 24:732–737

    Article  CAS  PubMed  Google Scholar 

  • Mousavi A, Hotta Y (2005) Glycine-rich proteins: a class of novel proteins. Appl Biochem Biotechnol 120:169–174

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Niessen N, Krause K, Horst I, Staebler N, Klaus S, Gaertner S, Kebeish R, Araujo WL, Fernie AR, Peterhansel C (2012) Two alanine aminotranferases link mitochondrial glycolate oxidation to the major photorespiratory pathway in Arabidopsis and rice. J Exp Bot 63(7):2705–2716

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    Article  CAS  PubMed  Google Scholar 

  • Novitskaya L, Trevanion SJ, Driscoll S, Foyer CH, Noctor G (2002) How does photorespiration modulate leaf amino acid contents? a dual approach through modelling and metabolite analysis. Plant, Cell Environ 25:821–835

  • Obata T, Fernie AR (2012) The use of metabolomics to dissect plant responses to abiotic stresses. Cell Mol Life Sci 69:3225–3243

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ohkama-Ohtsu N, Oikawa A, Zhao P, Xiang C, Saito K, Oliver DJ (2008) A γ-Glutamyl transpeptidase-independent pathway of glutathione catabolism to glutamate via 5-oxoproline in Arabidopsis. Plant Physiol 148:1603–1613

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Price KR, Howard B, Coxon DT (1976) Stress metabolite production in potato tubers infected by Phytophthora infestans, Fusarium avenaceum and Phoma exigua. Physiol Plant Pathol 9(2):189–197

    Article  CAS  Google Scholar 

  • Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB (1995) The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res 22(4):375–383

    Article  CAS  PubMed  Google Scholar 

  • Roessner U, Wagner C, Kopka J, Trethewey RN, Willmitzer L (2000) Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. Plant J 23(1):131–142

    Article  CAS  PubMed  Google Scholar 

  • Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signalling in plants: conserved and novel mechanisms. Annu Rev Plant Biol 57:675–709

    Article  CAS  PubMed  Google Scholar 

  • Sauter A, Davies WJ, Hartung W (2001) The long-distance abscisic acid signal in the droughted plant: the fate of the hormone on its way from root to shoot. J Exp Bot 52(363):1991–1997

    Article  CAS  PubMed  Google Scholar 

  • Schafleitner R, Gaudin A, Gutierrez R, Alvarado C, Bonierbale M (2007) Proline accumulation and real time PCR expression analysis of genes encoding enzymes of proline metabolism in relation to drought tolerance in andean potato. Acta Physiol Plant 29:19–26

    Article  CAS  Google Scholar 

  • Shelp BJ, Bown AW, McLean MD (1999) Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci 4(11):446–452

    Article  PubMed  Google Scholar 

  • Skirycz A, Inzé D (2010) More from less: plant growth under limited water. Curr Opin Biotechnol 21:197–203

    Article  CAS  PubMed  Google Scholar 

  • Smeekens S, Ma J, Hanson J, Rolland F (2010) Sugar signals and molecular networks controlling plant growth. Curr Opin Plant Biol 13:274–279

    Article  CAS  PubMed  Google Scholar 

  • Smillie RM, Hetherington SE (1999) Photoabatement by anthocyanin shilds photosynthetic system from light stress. Photosynthetica 36(3):451–463

    Article  CAS  Google Scholar 

  • Sperdouli I, Moustakas M (2012) Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress. J Plant Physiol 169:577–585

    Article  CAS  PubMed  Google Scholar 

  • Taji T, Chieko Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K (2002) Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant J 29(4):417–426

    Article  CAS  PubMed  Google Scholar 

  • Veselov DS, Mustafina AR, Sabirjanova IB, Akhiyarova GR, Dedov AV, Veselov SU, Kudoyarova GR (2002) Effect of PEG-treatment on the leaf growth response and auxin content in shoots of wheat seedlings. Plant Growth Regul 38:191–194

    Article  CAS  Google Scholar 

  • Wang H-L, Lee P-D, Liu L-F, Su J-C (1999) Effect of sorbitol induced osmotic stress on the changes of carbohydrate and free amino acid pools in sweet potato cell suspension cultures. Bot Bull Acad Sin 40:219–225

    CAS  Google Scholar 

  • Wingler A (2002) Molecules of interest. The function of trehalose biosynthesis in plants. Phytochemistry 60:437–440

  • Winkel-Shirley B (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol 5:218–223

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Gou M, Liu C-J (2013) Arabidopsis kelch repeat F-Box proteins regulate phenylpropanoid biosynthesis via controlling the turnover of phenylalanine ammonia-lyase. Plant Cell 25:4994–5010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

The study was conducted with funding provided by the (BMEL) through the Agency of Renewable Resources (FNR e.V.), funding no Federal Ministry of Food and Agriculture: 22023511. The authors would like to thank Birgit Lippmann for GC/MS support and Dr. A. Schum for in vitro cultures of the studied potato genotypes.

Authors contribution

C. Bündig: Planned and performed experiments, analysed data, prepared all figures and wrote the manuscript. C. Blume: Standard curve preparation, manuscript correction and final approval. C. Peterhänsel: Helped in experimental design, manuscript correction and final approval. T. Winkelmann: Helped in planning experiment, contribution to writing and correction of the manuscript and final approval.

Author information

Authors and Affiliations

  1. Institute for Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hannover, Germany

    Christin Bündig & Traud Winkelmann

  2. Institute for Botany, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hannover, Germany

    Christian Blume & Christoph Peterhänsel

Authors
  1. Christin Bündig
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christian Blume
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christoph Peterhänsel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Traud Winkelmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Traud Winkelmann.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bündig, C., Blume, C., Peterhänsel, C. et al. Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up?. Plant Cell Tiss Organ Cult 127, 195–206 (2016). https://doi.org/10.1007/s11240-016-1042-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-016-1042-1

Keywords

Search

Navigation