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Callus cultures of Hypericum perforatum L. a novel and efficient source for xanthone production

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Abstract

Hypericum perforatum callus cultures exhibited qualitatively and quantitatively different phenolic profile compared to wild-growing plants (WGP). This study revealed the presence of phenolic acids, flavonoid glycosides and aglycones, flavan-3-ols and naphtodianthrones in callus and WGP extracts. Phloroglucinols were detected only in WGP, while xanthones were exclusively found in callus cultures. Among the caffeoyl-, coumaroyl- and feruloyl-quinic acid derivatives identified in both extracts, 3-feruloylquinic acid was identified only in callus cultures. Flavonoid glycosides were represented with quercetin and kaempferol derivatives in both extracts, whereas calli produced a new flavone of luteolin type. Callus cultures showed superior potential for the production of quercetin 6-C-glucoside, hyperoside and kaempferol 3-O-glucoside compared to WGP. Flavonoid aglycones, quercetin and kaempferol were found in both extracts, while I3,II8-biapigenin was accumulated only in WGP. Catechin and procyanidin dimers were abundant in WGP, while epicatechin was dominant flavan-3-ol in callus cultures. With respect to naphtodianthrones, undifferentiated calli were able to produce only pseudohypericin, but in minor amounts compared to WGP. Among the eighteen detected xanthones, mangiferin, mangiferin C-prenyl hexoside and 1,3,6,7-tetrahydroxyxanthone were found as the major compounds in callus cultures. Four xanthones identified as roeperanone, 1,3,7-trihydroxy-2-(2-hydroxy-3-methyl-3-butenyl)-xanthone, trihydroxy-1-methoxy C-prenyl xanthone and 1,5-dihydroxy-2-methoxyxanthone were shown for the first time in H. perforatum callus extracts. Altogether, these results indicated that H. perforatum callus cultures represent a promising experimental system for the production of novel xanthone derivatives and other bioactive metabolites of commercial importance.

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Authors and Affiliations

  1. Department of Plant Physiology, Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Archimedova str. 3, 1000, Skopje, Macedonia

    Oliver Tusevski, Elizabeta Markoska, Natasa Brndevska & Sonja Gadzovska Simic

  2. Department of Analytical Chemistry, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Archimedova str. 3, 1000, Skopje, Macedonia

    Jasmina Petreska Stanoeva & Marina Stefova

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  1. Oliver Tusevski
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  2. Jasmina Petreska Stanoeva
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  3. Elizabeta Markoska
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  4. Natasa Brndevska
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  5. Marina Stefova
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  6. Sonja Gadzovska Simic
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Correspondence to Sonja Gadzovska Simic.

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Tusevski, O., Stanoeva, J.P., Markoska, E. et al. Callus cultures of Hypericum perforatum L. a novel and efficient source for xanthone production. Plant Cell Tiss Organ Cult 125, 309–319 (2016). https://doi.org/10.1007/s11240-016-0951-3

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  • DOI: https://doi.org/10.1007/s11240-016-0951-3

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