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Responses of grass pea seedlings to salinity stress in in vitro culture conditions

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Abstract

Physiological and molecular mechanisms of adaptation to abiotic stresses of grass pea (Lathyrus sativus L.) are still poorly understood. Responses of four genotypes of grass pea to salinity stress in tissue culture conditions were investigated at early seedling growth stages. Salinity stress was induced in the agar media by adding 0, 50, 100 and 200 mM of NaCl. Germination and seedling emergence percentage was not significantly affected by 50 and 100 mM of NaCl. However, NaCl in 200 mM concentration lowered level of these parameters. Generally, exposure to NaCl stress significantly reduced length of grass pea seedling organs (root and shoot) but did not influence the content of dry weight in shoots and increased it in the roots in two cases. Increasing salt concentration decreased integrity of cellular membranes both in root and shoot tissues. Higher accumulation of phenolic compounds and significant changes in activity of antioxidant enzymes (peroxidase and catalase) were observed in the roots but not in the shoots. Similarly, the content of proline increased mostly in the roots from moderate (100 mM) salinity conditions. Adverse conditions did not resulted in alterations in photosynthetic pigments content of any tested genotypes. The better performance of shoots than roots may result from in vitro conditions in which experiments were conducted.

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Acknowledgments

This Research was financed by the Ministry of Science and Higher Education of the Republic of Poland (Project No. BM4569).

Author contributions

B.P. designed and performed experiments, analysed data and wrote the paper; K.T. discussed the results and wrote the paper; I.K. gave technical support and performed experiments.

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  1. Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland

    Barbara Piwowarczyk, Krzysztof Tokarz & Iwona Kamińska

  2. The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland

    Krzysztof Tokarz

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  1. Barbara Piwowarczyk
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Correspondence to Barbara Piwowarczyk.

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Piwowarczyk, B., Tokarz, K. & Kamińska, I. Responses of grass pea seedlings to salinity stress in in vitro culture conditions. Plant Cell Tiss Organ Cult 124, 227–240 (2016). https://doi.org/10.1007/s11240-015-0887-z

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