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Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro

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Abstract

The plant hormones cytokinins (CKs) regulate a number of physiological processes. Their homeostasis is controlled by the rate of de novo synthesis and the rate of catabolism. The aim of this work was to analyze the content of total as well as individual groups of endogenous CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable model system for studying physiological and morphological processes controlled by CKs. In this work we clearly demonstrate a significant effect of AtCKX transgenes on CK metabolism in transgenic centaury plants. However, shoots and roots of only one AtCKX1 line and three AtCKX2 lines with a significant reduction of bioactive CKs were obtained. We also show that changes in the CKs metabolism considerably affected endogenous indole-3-acetic acid (IAA) levels in plant tissues. All analyzed transgenic AtCKX centaury lines exhibited decreased amount of endogenous IAA in shoots as well as in roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation in the shoots and roots of all analyzed AtCKX centaury transformants.

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Abbreviations

CKs:

Cytokinins

CKX:

Cytokinin oxidase/dehydrogenase

IAA:

Indole-3-acetic acid

IPT:

Isopentenyltransferase

MS:

Murashige and Skoog medium

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Acknowledgments

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. ON173015) and the Czech Science Foundation (P506/11/0774).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade, Serbia

    Milana Trifunović, Aleksandar Cingel, Angelina Subotić, Slađana Jevremović & Marija Petrić

  2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 16502, Prague 6, Czech Republic

    Václav Motyka, Jiří Malbeck & Petre I. Dobrev

  3. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 14220, Prague 4, Czech Republic

    Josef Holík

  4. Faculty of Biology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia

    Ivana Č. Dragićević

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  1. Milana Trifunović
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  2. Václav Motyka
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  3. Aleksandar Cingel
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  4. Angelina Subotić
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  5. Slađana Jevremović
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  7. Josef Holík
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  9. Petre I. Dobrev
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  10. Ivana Č. Dragićević
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Correspondence to Milana Trifunović.

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Milana Trifunović and Václav Motyka have contributed equally to this work.

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Trifunović, M., Motyka, V., Cingel, A. et al. Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants grown in vitro. Plant Cell Tiss Organ Cult 120, 767–777 (2015). https://doi.org/10.1007/s11240-014-0646-6

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