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Somatic embryogenesis and in vitro plantlet regeneration in Salicornia brachiata Roxb.

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Abstract

Salicornia brachiata Roxb. (Amaranthaceae) a leaf-less annual succulent halophyte, grows under extremely saline conditions and is an important resource for salt stress responsive genes. Here we report somatic embryogenesis and in vitro plantlet regeneration in S. brachiata for the first time. In vitro-grown seedlings were used as explants and 86.0 ± 1.7 % explants produced embryogenic callus on 0.75 % agar-gelled Murashige and Skoog’s (MS) medium containing 2.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-d) in 16 weeks. Somatic embryogenesis was achieved on MS medium supplemented with 0.25 mg l−1 2,4-d. On this medium sustained cell division and growth within the first 8 weeks resulted in the formation of cell aggregates in 73.65 ± 0.44 % cultures producing globular somatic embryos (SEs). Gradually these converted into heart, torpedo and cotyledonary shaped SEs following subsequent 16 weeks subculture on the same medium. Around 35 % SEs germinated on plant growth regulator free MS medium. The somatic seedlings were acclimatized on sterile soil with 40–45 % survival.

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Fig. 1

Abbreviations

2,4-d :

2,4-dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

PGR:

Plant growth regulator

RT:

Room temperature

SFP:

Spectral flux photon

GMO:

Genetically modified organism

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Acknowledgments

Authors acknowledge the financial support from CSIR, New Delhi, Govt. of India for establishment of tissue culture facilities. N. Paliwal is thankful to CSIR, New Delhi for financial assistance. CSIR-CSMCRI communication no. PRIS/155/2013.

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Authors and Affiliations

  1. Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research, G.B. Marg, Bhavnagar, 364002, Gujarat, India

    Mangal S. Rathore, Nikita Paliwal, K. G. Vijay Anand & Pradeep K. Agarwal

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  1. Mangal S. Rathore
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  2. Nikita Paliwal
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  3. K. G. Vijay Anand
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  4. Pradeep K. Agarwal
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Correspondence to Mangal S. Rathore or Pradeep K. Agarwal.

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Rathore, M.S., Paliwal, N., Anand, K.G.V. et al. Somatic embryogenesis and in vitro plantlet regeneration in Salicornia brachiata Roxb.. Plant Cell Tiss Organ Cult 120, 355–360 (2015). https://doi.org/10.1007/s11240-014-0571-8

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