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The TrAP/REn monodirectional promoter of Mungbean yellow mosaic geminivirus (MYMV) displays root-specific expression in transgenic tobacco

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Abstract

The TrAP/REn monodirectional promoter of Mungbean yellow mosaic virus (MYMV) comprises many root-specific motifs. The TrAP/REn promoter fused to the β-glucuronidase (gus) reporter gene was used to transform tobacco. Histochemical staining of various parts of seven transgenic plants showed a preferential root-specific expression pattern. Tobacco transformation with the TrAP gene under the transcriptional control of the TrAP/REn promoter yielded nine transgenic plants, of which six harboured the complete TrAP gene in the integrated T-DNAs. Transcript analysis indicated root tissue-specific expression of the TrAP gene. TrAP transgenic plants were phenotypically normal. Expression of the TrAP gene under its own promoter obviated the toxicity which was observed when the TrAP gene was expressed under the CaMV 35S promoter.

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Abbreviations

Rep:

Replication-associated protein

TrAP:

Transcriptional activator protein

REn:

Replication enhancer protein

CP:

Coat protein

MP:

Movement protein

NSP:

Nuclear shuttle protein

IGR:

Intergenic region

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Acknowledgments

We are thankful to Dr. K. Dharmalingam, Madurai Kamaraj University for permitting us to use the Liquid Scintillation Counter. This study was financially supported by the Department of Biotechnology (Project Number-BT/PR7866/AGR/02/379/2006), Government of India. S.S acknowledges the Council of Scientific and Industrial Research, Government of India for the research fellowship.

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  1. Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625021, India

    Sukumaran Sunitha, Nagrani Mahajan & Karuppannan Veluthambi

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  1. Sukumaran Sunitha
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  2. Nagrani Mahajan
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  3. Karuppannan Veluthambi
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Correspondence to Karuppannan Veluthambi.

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Sunitha, S., Mahajan, N. & Veluthambi, K. The TrAP/REn monodirectional promoter of Mungbean yellow mosaic geminivirus (MYMV) displays root-specific expression in transgenic tobacco. Plant Cell Tiss Organ Cult 109, 535–545 (2012). https://doi.org/10.1007/s11240-012-0120-2

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