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Infectivity analysis of two variable DNA B components ofMungbean yellow mosaic virus-Vigna inVigna mungo andVigna radiata

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Abstract

Mungbean yellow mosaic virus-Vigna (MYMV-Vig), aBegomovirus that causes yellow mosaic disease, was cloned from field-infected blackgram (Vigna mungo). One DNA A clone (KA30) and five different DNA B clones (KA21, KA22, KA27, KA28 and KA34) were obtained. The sequence identity in the 150-nt common region (CR) between DNA A and DNA B was highest (95%) for KA22 DNA B and lowest (85·6%) for KA27 DNA B. The Rep-binding domain had three complete 11 -nt (5’-TGTATCGGTGT-3′) iterons in KA22 DNA B (and KA21, KA28 and KA34), while the first iteron in KA27 DNA B (5’-ATCGGTGT-3’) had a 3-nt deletion. KA27 DNA B, which exhibited 93·9% CR sequence identity to the mungbean-infecting MYMV, also shared the 3-nt deletion in the first iteron besides having an 18-nt insertion between the third iteron and the conserved nonanucleotide. MYMV was found to be closely related to KA27 DNA B in amino acid sequence identity of BV1 (94·1%) and BC1 (97·6%) proteins and in the organization of nuclear localization signal (NLS), nuclear export signal (NES) and phosphorylation sites. Agroinoculation of blackgram (V. mungo) and mungbean (V. radiata) with partial dimers of KA27 and KA22 DNA Bs along with DNA A caused distinctly different symptoms. KA22 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in blackgram. In contrast, KA27 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in mungbean. Thus, DNA B of MYMV-Vig is an important determinant of host-range betweenV. mungo andV. radiata.

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Abbreviations

CR:

Common region

ds:

double-stranded

kb:

kilobase pair(s)

kDa:

kiloDalton(s)

lin:

linear

MYMV-Vig:

Mungbean yellow mosaic virus-Vigna

NES:

nuclear export signal

NLS:

nuclear localization signal

nt:

nucleotide

oc:

open circular

OD:

optical density

ORF:

open reading frame

Rep:

replication-associated protein

RF:

replicative form

sc:

supercoiled

ss:

single-stranded

YMD:

yellow mosaic disease

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Author notes

  1. R. Vanitharani

    Present address: Donald Danforth Plant Sciences Center, ILTAB, 63132, St Louis, Missouri, USA

  2. A. S. Karthikeyan

    Present address: Department of Horticulture and Landscape Architecture, Purdue University, 47907, West Lafayette, Indiana, USA

Authors and Affiliations

  1. Centre for Plant Molecular Biology and Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, 625 021, Madurai, India

    V. Balaji, R. Vanitharani, A. S. Karthikeyan, S. Anbalagan & K. Veluthambi

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  1. V. Balaji
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  2. R. Vanitharani
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  3. A. S. Karthikeyan
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  4. S. Anbalagan
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  5. K. Veluthambi
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Correspondence to K. Veluthambi.

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Balaji, V., Vanitharani, R., Karthikeyan, A.S. et al. Infectivity analysis of two variable DNA B components ofMungbean yellow mosaic virus-Vigna inVigna mungo andVigna radiata . J Biosci 29, 297–308 (2004). https://doi.org/10.1007/BF02702612

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