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Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars

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Abstract

Shoot organogenesis from mature leaf tissues of two scented Pelargonium capitatum cultivars, ‘Attar of Roses’ and ‘Atomic Snowflake’, grown in the greenhouse, were optimized in the presence of thidiazuron (TDZ). The protocol involved preculture of leaf sections on basal Murashige and Skoog (MS) medium supplemented with 10 μM TDZ, 4.4 μM of 6-benzyladenine (BA) and 5.4 μM α-naphtaleneacetic acid (NAA) for a period of 2 weeks and followed by subculture of explants to a fresh medium containing 4.4 μM BA and 5.4 μM NAA. Frequency of regeneration reached approximately 93% for both cultivars, with the induction of more than 100 shoots per explant. Regenerated plantlets were rooted on half-strength MS medium supplemented with 4.4 mM sucrose and 8.6 μM of Indole-3-acetic acid (IAA). All regenerated shoots from both cultivars developed roots when transferred to organic soil mix, acclimatized, and successfully transferred to greenhouse conditions. When regenerated shoots were transferred to hydroponic conditions, frequency of survival was 76.2 and 61.9% for ‘Attar of Roses’ and ‘Atomic Snowflake’, respectively.

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Acknowledgments

The authors would like to thank the Higher Education Commission of Pakistan (www.hec.gov.pk) for sponsoring M. Arshad. We are also thankful to Prof. (Emeritus) Gilbert Alibert for helpful discussions.

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Author notes

  1. M. Arshad

    Present address: Institute of Environmental Sciences and Engineering (IESE-SCEE), National University of Sciences and Technology (NUST), H-12, Islamabad, 44000, Pakistan

Authors and Affiliations

  1. Université de Toulouse, INP-ENSAT, Ave. de l’Agrobiopôle, BP32607, 31326, Auzeville Tolosane, Castanet-Tolosan, France

    M. Arshad, J. Silvestre, G. Merlina, C. Dumat, E. Pinelli & J. Kallerhoff

  2. UMR 5245 CNRS-INPT-UPS, EcoLab (Laboratoire Ecologie Fonctionnelle et Environnement), Ave. de l’Agrobiopôle, BP32607, 31326, Auzeville Tolosane, Castanet-Tolosan, France

    M. Arshad, J. Silvestre, G. Merlina, C. Dumat, E. Pinelli & J. Kallerhoff

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  1. M. Arshad
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  2. J. Silvestre
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  3. G. Merlina
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  4. C. Dumat
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  5. E. Pinelli
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  6. J. Kallerhoff
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Correspondence to J. Kallerhoff.

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Arshad, M., Silvestre, J., Merlina, G. et al. Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars. Plant Cell Tiss Organ Cult 108, 315–322 (2012). https://doi.org/10.1007/s11240-011-0045-1

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  • DOI: https://doi.org/10.1007/s11240-011-0045-1

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