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Optimisation of plantlet regeneration from leaf and nodal derived callus of Vanilla planifolia Andrews

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Abstract

An indirect in vitro plant regeneration protocol for Vanilla planifolia has been established. Juvenile leaf and nodal segments from V. planifolia were used as explants to initiate callus. Nodal explants showed better callus initiation than juvenile leaf explants, with 35.0% of explants forming callus when cultured on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg/l 1-naphthylacetic acid (NAA) and 1.0 mg/l 6-benzyladenine (BA). Almost 10.0% of juvenile leaf explants were induced to form callus on the MS basal medium containing 2.0 mg/l NAA and 2.0 mg/l BA, whereas no callus formed in the presence of any concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and BA. After 8 weeks, callus generated was transferred to MS basal medium containing 1.0 mg/l BA and 0.5 mg/l NAA. A mean number of 4.2 shoots per callus was produced on this medium, with a mean length of 3.8 cm after 8 weeks of culture. Roots formed on 88.3% of plantlets when they were cultured on MS medium supplemented with 1.0 mg/l NAA, with a mean length of 4.4 cm after 4 weeks of culture. Of the rooted plantlets, 90.0% survived acclimatisation and were making new growth after 4 weeks.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

1-Naphthylacetic acid

KIN:

Kinetin

BA:

6-Benzyladenine

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

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Acknowledgements

This research was supported by an eScience Fund from the Ministry of Science, Technology and Innovation (MOSTI), Malaysia.

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Authors and Affiliations

  1. School of Biosciences, Faculty of Science, The University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia

    Boon Chin Tan, Chiew Foan Chin & Peter Alderson

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  1. Boon Chin Tan
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  2. Chiew Foan Chin
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  3. Peter Alderson
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Correspondence to Chiew Foan Chin.

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Tan, B.C., Chin, C.F. & Alderson, P. Optimisation of plantlet regeneration from leaf and nodal derived callus of Vanilla planifolia Andrews. Plant Cell Tiss Organ Cult 105, 457–463 (2011). https://doi.org/10.1007/s11240-010-9866-6

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  • DOI: https://doi.org/10.1007/s11240-010-9866-6

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