Abstract
Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l–1. Medium containing 200 ml ⋅ l–1 coconut water together with 40 g ⋅ l–1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
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Received: 11 June 1997 / Revision received: 6 October 1997 / Accepted: 18 October 1997
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Ishii, Y., Takamura, T., Goi, M. et al. Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Reports 17, 446–450 (1998). https://doi.org/10.1007/s002990050423
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DOI: https://doi.org/10.1007/s002990050423